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Description
Annexin V-EGFP/PI Apoptosis analysis kitProduct Specification Usage 1. Adjust the cell density to about 1 106Cells ml. 2. Remove 0. 2 ml of cell suspension from a cell culture flask (2 105Cells) were transferred into a clean centrifuge tube. 3. Centrifuge at 1000 g at room temperature for 5 minutes to remove the medium. 4. The cell pellet was gently resuspended with 0. 2 ml of pre cooled PBS solution. 5. Centrifuge at 1000 g for 5 minutes at room temperature to remove PBS. 6. The cell
Product Specification
| Usage | 1. Adjust the cell density to about 1 × 106Cells/ml. 2. Remove 0.2 ml of cell suspension from a cell culture flask (2 × 105Cells) were transferred into a clean centrifuge tube. 3. Centrifuge at 1000 g at room temperature for 5 minutes to remove the medium. 4. The cell pellet was gently resuspended with 0.2 ml of pre-cooled PBS solution. 5. Centrifuge at 1000 g for 5 minutes at room temperature to remove PBS. 6. The cell pellet was gently resuspended with 0.2 ml of pre-cooled binding buffer (component C. Binding Buffer). 7. Add 5μl Annexin V-EGFP (component A) and 5μl PI dye solution (component B), and gently mix well. 8. Incubate at room temperature (18-24 °C) for 10-15 minutes in the dark. 9. Alternatively, replenish the volume to 0.4 ml with binding buffer or PBS. 10. Place the sample on ice and protect it from light, and test it as soon as possible. 11. Use flow cytometry or fluorescence microscope for detection and analysis. | ||||||||||||||||
| Beilstein | 0 | ||||||||||||||||
| Synonym | Annexin V-EGFP/PI Apoptosis Analysis Kit | ||||||||||||||||
| Description |
Annexin V-EGFP/PI cell apoptosis detection kit uses EGFP-labeled Annexin V as a probe to detect the occurrence of early cell apoptosis. The detection principle is: in normal living cells, phosphotidylserine (PS) is located on the inside of the cell membrane, but in early apoptotic cells, PS flips from the inside of the cell membrane to the surface of the cell membrane and is exposed to the extracellular environment. Annexin-V (Annexin-V) is a Ca2 +-dependent phospholipid binding protein with a molecular weight of 35-36KD, which can bind to PS with high affinity. It can bind to the cell membrane of early apoptotic cells through phosphatidylserine exposed on the outside of the cell. In addition, Propidium Iodide (PI) is also provided in this kit to distinguish surviving early cells from necrotic or late apoptotic cells. PI is a nucleic acid dye that cannot penetrate the intact cell membrane of normal cells or early apoptotic cells, but can penetrate the cell membrane of late apoptotic and necrotic cells to make the nucleus red. Therefore, when Annexin V is used in combination with PI, PI is excluded from viable cells (Annexin V-/PI-) and early apoptotic cells (Annexin V +/PI-), while late apoptotic cells and necrotic cells are stained double positive by EGFP and PI combination (Annexin V +/PI +).
Product Components:
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| PubChem CID | 0 | ||||||||||||||||
| Storage Temp. | Stored at 2-8 ℃ in the dark from light, the validity period is 6 months. Store at-20 ℃ in the dark from light, shelf life for 12 months or more, avoid repeated freezing and thawing. |
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4.3 ★★★★★
Based on 30 reviews
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Product Reviews
★★★★★ 5
High quality, durable, loved by my chewer *
Size: Medium (Pack of 1), Style: Stick Medium
Excellent chew bone Toy for aggressive chewer. I've purchased this same brand bone/ Toy for several years. One lasts at least 1-2 years ( if it doesn't get run over by a lawn mower). Lol. I usually pay $8-$9 for one in retail stores. This is an excellent price at $6.
Squeaker stops after a few months but my Field Spaniel dog is deaf now anyway! He loves chewing on this for his gums and when people are gathered in the same room he just starts chewing and enjoying himself in the midst of fellowship.
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Reviewed in the United States on October 31, 2024
★★★★★ 1
Not for aggressive chewers
Size: Large (Pack of 1), Style: Stick Large, Size: Large (Pack of 1), Style: Stick Large
I got this for my 95lb lab who is an aggressive chewer thinking that it looked durable and would last a while. He tore it apart within an hour and I had to throw it away.
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Reviewed in the United States on April 14, 2026
★★★★★ 3
Felt durable but it couldn’t hold up
Size: Large (Pack of 1), Style: Stick Large, Size: Large (Pack of 1), Style: Stick Large
I really thought my pet wouldn’t be able to destroy it but it’s in tiny pieces in the trash now. we played together and she was gentle I guess because she didn’t bite or tug hard at all I left her alone with it like I’ve done before but this time she broke it down in chunks. Did not have this toy for more then 24 hours.
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Reviewed in the United States on January 20, 2025
★★★★★ 5
Can be tossed around indoors
Size: Large (Pack of 1), Style: Stick Large
Flexible stick toy. Dogs enjoy carrying it around. Squeaks. We have had one for years. It is still in great shape but bought 2 more as I have 3 dogs and all enjoy them. I can toss this in the house.
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Reviewed in the United States on May 18, 2026
★★★★★ 5
Great fun & strong!!!
Size: Large (Pack of 1), Style: Stick Large
These are the best sticks ever!
Our dogs chew and slobber all over these for sometimes an hour at a time. They like the “give” texture when they chew it. Imagine an indestructible, gummy bear!
Because of their softness, they don’t end up with sharp edges or texture, although they are sturdy, and very durable. We have had these for our two dogs for close to a month now and it is their go to indoor to stick. The gentle squeak is not loud and obnoxious, but a playful sound that has them coming back for more with each chomp! If you love your dogs, this is the cheese stick for them.
He’s also make an excellent fetch stick as they can go inside or outside. They wash off easily with water.
This is a great investment, especially if you’ve been wasting your money replacing toys for strong jawed dogs!
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Reviewed in the United States on March 22, 2023