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Description
Mouse ITGa1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Preparation of standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 5000pg/mL). Then dilute to the following concentrations: 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312.5pg/mL, 156.25pg/mL, 78.125pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 5000pg/mL standard working solution into the first EP tube and mix thoroughly to make a 2500pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an Integrin Alpha 1 (ITGa1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Integrin Alpha 1 (ITGa1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Integrin Alpha 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Integrin α-1, also known as CD49a, is an integrin α subunit encoded by the gene ITGA1. It forms one half of the α1β1 integrin doublet. Diseases associated with ITGA1 include prepuce and intestinal carcinoma in situ. Pathways associated with ITGA1 include VEGFR3 signaling in the lymphatic endothelium and TCPTP-mediated signaling events. Gene Ontology annotations associated with this gene include signaling receptor binding and collagen binding. An important homolog of this gene is ITGA2. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 78.12-5000pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.7 ★★★★★
Based on 22 reviews
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Product Reviews
★★★★★ 2
Strong well made but ..
Color: Gold
Coring tool broke as soon as I tried to loosen the top. As far as a tube goes it is great. Seals tight, solid Aluminum throughout. You could throw this off a tall building and it would be fine afterwards.
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Reviewed in the United States on April 26, 2026
★★★★★ 5
I love this thing!
I can't overstate how much I love this thing! As someone who enjoys a good cigar on the go, I've tried various single cigar cases, but this one is in a league of its own. First, the build quality is outstanding. The aluminum alloy feels incredibly solid and durable in your hand. It's clear this was made to last. I have no doubt that my cigar is perfectly protected from being crushed or damaged when it's in this thing. The convenience factor is a 10/10. It’s sleek, compact, and makes carrying a single cigar effortless.
But it's the brilliant integration of accessories that, for me, truly sets it apart. Having a sharp, reliable punch built right in is fantastic. The built-in hygrometer gives me total peace of mind that my cigar is staying perfectly fresh, and the clever design that allows the top to double as a cigar holder is just plain genius. This tube is the perfect, all-in-one solution for any cigar aficionado who travels or simply likes to be prepared. I'd recommend it to anyone without a moment's hesitation. Five stars all the way!
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Reviewed in the United States on August 28, 2025
★★★★★ 5
Nice light saber handle with some room for a cigar lol
I'll go straight to the point: this cigar tube is great! I really like the material, it's made of thick aluminium and it's design is both classy and nerdy at the same time, it looks like a jedi light saber handle lol
The hygrometer seems to be accurate, close to my other ones, it's a nice extra functionality.
The size of the tube is big enough for my largest cigars, so all good here.
I recommend it, either as a gift to yourself (because you deserve it!), or father's day, or any good occasion!
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Reviewed in the United States on August 20, 2025
★★★★★ 4
does the job!
I bought the DXXULL 4-in-1 Cigar Tube as a gift for my husband, and overall, it’s been a solid choice. He travels often and enjoys cigars, so this compact, all-in-one tube really suits his lifestyle. The aluminum alloy feels sturdy and well-made, and the built-in cigar punch, hygrometer, and holder add a nice touch of convenience. It’s sleek and functional—he’s used it several times already without any issues. While I do think it’s a bit overpriced for its size, the quality and thoughtful design make up for it. If you’re looking for a practical, stylish gift for a cigar lover, this tube does the job well.
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Reviewed in the United States on October 30, 2025
★★★★★ 3
won't fit Churchill length or 60-ring diameter cigars
Color: Gold, Color: Gold
This is a good-looking device, solidly built, with a handy twist-out punch in the top and a humidity gauge in the bottom. The hygrometer is pointless, because there is no way to add anything to actually maintain the humidity, and since I would only use it to bring a cigar to a gathering, it really does not need to maintain that humidity for the 20-minute drive.
What really bugs me about this is the actual useable size. I primarily smoke 2 types of cigars, 54-ring Churchills (which are 7") and 60-ring Gorditos (which are 6"). Although the overall length of this is 8 1/4", the listing never mentions the interior space, which seems to be just under 6 1/2" when closed. My Montecristo sticks out about 1/4" when I insert it, and that does not take into account the 1/4" that the top pushes into when closed (that silver-colored area is solid), thus less than 6 1/2" useable length. Even out of the wrapper, my 60-ring Gorditos sticks when I try to push it in, so I'd have to take off the band(s) to force it in, and it would not slide out very easily, so I would mangle the cigar trying to get it out. So, this can hold a cigar that is 6" or less and maybe 54-ring without the wrapper. Anything else, and you would have to force it in and force it out. So, this unit, while very good looking and well built, does not work for my cigar needs.
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Reviewed in the United States on June 13, 2025