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Description
Human PSEP ELISA KitProduct Specification Usage Experimental equipment required: 1. Microplate reader (450nm) 2. High precision pipette and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C constant temperature box 4. Distilled or deionized water Sample preparation and requirements: UrineOther biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre test preparation: 1. Remove the test kit from the refrigerator 10 minutes
Product Specification
| Usage |
Experimental equipment required: 1. Microplate reader (450nm) 2. High-precision pipette and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C constant temperature box 4. Distilled or deionized water Sample preparation and requirements: UrineOther biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a prostatic exosomal protein (PSEP) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of prostatic exosomal protein (PSEP) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human prostatic exosomal protein ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Prostatic exocytic protein (PSEP) is a small protein secreted by the prostate. PSEP effectively inhibits viral activity and also acts as an antibacterial agent, protecting sperm and enhancing sperm motility. When the prostate is inflamed, this protein is released into the urine, allowing for detection. It is an important tool for diagnosing chronic nonbacterial prostatitis. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Urine and other biological fluids |
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4.8 ★★★★★
Based on 10 reviews
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Product Reviews
★★★★★ 5
Very comfortable with good lower-back support
Most comfortable, well made, easy assembly office-gaming chair (very pleased)!
*I suggest, HOLD-OFF popping the seat onto the upper-cylinder until AFTER assembling the back-rest onto the seat/arm-seat with back-rest as a unit...can then be popped onto the upper gas-cylinder(Much more stable than fighting with chair freely rolling and turning) while attempting to aligning the holes assembling the back-rest.
Afterwards the seat with back-rest assembled can easily be popped onto to the upper-end of the gas-cylinder and we are good to go!
* do pay attention to using the correct bubble-packed bolts for each assembly, as directed
* all the holes align -up very precisely... so if screwing in the bolt(s) and after a couple-turns it gets hard-to-turn, we are 'cross-threading the bolt into the threaded hole...so back-out and realign the bolt as so it threads in very easy.
* the bubble-pack of bolts are clearly labeled so as we easily know which ones go where.
*0ne bubble-packet even includes a spare group just in case we lost one (like out in the grass-lawn)?
* Included a T-handle alien-head wrench (all needed for total assembly) very nice for having around if any re-tightening needed. all speaks Quality!
>>> Been using about a month now...And I REALLY like this office gaming chair
_ very comfortable cushioned well and excellent lower-back support
_ the faux-leather seems to be more thick and durable and stitching is NOT ripping-out (as my previous & more expensive chair had done with it's paper-thin faux leather)
_ easy assembled chair seems very sturdy and solid, (yet light weight), in moving around, (not some wobbly contraption as my previous more expensive chair).
_ a strong Five-Star rating!
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Reviewed in the United States on March 14, 2025
★★★★★ 5
nice chair for the money
This is a great chair for those not wanting to spend a lot of money and well built and sturdy. I don't do gaming so I'm looking at it as a computer chair so that's what my review will focus on. I had no trouble putting it together as it has a nice instruction book and not a one page print out like most China made products. Also the enclosed Allan wrench with a handle was someone's smart idea. It looks good (I also bought it since it was red) it's comfortable and fit's my needs but I can't comment on the durability factor as I have it for just a few days but I have no complaints for the price I paid at this time.
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Reviewed in the United States on November 19, 2024
★★★★★ 5
I Recommend!
very comfortable chair ive had it for a while now, it starts to get a faint creaking noise, but nothing unbearable. the cushions are firm yet comfortable at the same time. its not too big of a chair, fits great in my room. for the price , its pretty good quality, same could be said for its reclining, depending on how you set the tension of the reclining, the chair can be reclined with ease, or tight. this chair was easy to use and to set up. I very much recommend.
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Reviewed in the United States on September 13, 2025
★★★★★ 5
Great chair at a great price point.
This item was easy to put together, it was comfortable and visually stunning to look at. My toddlers are able to climb in and out as the chair remains stable. It’s simple to recline, and does so smoothly. The quality is great especially for the price point
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Reviewed in the United States on July 7, 2025
★★★★★ 4
I would by again!
Good and lasted for over a year. A good quality product.
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Reviewed in the United States on April 11, 2026