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Description
Human RAB5C ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against the Ras-related protein Rab-5C (RAB5C). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Ras-related protein Rab-5C (RAB5C) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Ras-related protein Rab-5C ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | RAB5C (RAB5C), a member of the RAS oncogene family, also known as Ras-associated protein Rab-5C, is a protein encoded by the RAB5C gene. It belongs to the Ras-associated protein family and is a small GTPase in the Ras superfamily. It is believed to ensure the fidelity of vesicle docking and/or fusion with its correct receptor compartment. It regulates Rac-mediated cell motility and is a key regulator of the trafficking of the CD93/Multimerin-2/β1 integrin complex in endothelial cell adhesion and migration. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.4 ★★★★★
Based on 16 reviews
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Product Reviews
★★★★★ 4
These are very nice but limited life
Size: 1pack, Color: Black, Size: 1pack, Color: Black
I got a couple of these for my wife's iphone 8+.
They really are great in all charging respects when fed with a high power supply.
But be aware they probably will not have long life. It depends on how often you plug/unplug the charger cord, and how you manhandle the charger itself.
What I hate about these is the use of the USB micro charging port.
If Anker were to use a USB type C charging port -maybe the lifespan would be greater.
But I doubt it. This is why a 5 star item becomes a 3 star.
The problem with USB ports as a power connector is that most people are rather klutzy with this connector while plugged into the charger's port. The USB micro connector socket is a very flimsy structure and not suited to heavy use (many plug/unplug or move cycles).
If you are clumsy with plugging in the cable, you bend the outer metal housing that both guides the connector in while plugging, and also holds fast the connector so the contacts mate and remain in contact. Same is true if you move the charger body...it flexes the power cord which in turn wobbles the connector.
Another great way to mangle the connector is moving or dropping the charger's body while the cord is connected. Anything that wobbles the cord while connected tends to waller out the USB port and after enough slop, the charger can become inoperative.
Once the geometry of the housing is compromised, the electrical power connection becomes flaky (intermittent) and then you have a charger that may or may not work. So then you have a phone without the expected recharge. For most people this forces them to discard and re-purchase the charger.
Call it designed-in obsolescence (shortened lifespan).
I have seen this across a wide range of electrical items. Anything that uses the USB mini or micro port as an electrical path for the sole purpose of energy transference will eventually fail. USB type A (large rectangular) is less affected by this due to the heavier, robust structure.
For me, I repair these by simply installing a permanently connected power cord. Soldered directly to the charger's circuit board. This is not something most people can do, and it requires intrusive access to the charger. On this model there are no screws to gain access to the connector and PCB...the case is permanently glued together.
If Anker wanted to provide a better solution, they would deploy these with a barrel connector/power pack, but they play this game with the consumer forcing them to buy a separate, high-power pack that must be USB.
bleah!
Update 10 July 2023
Anker was graceful in contacting me the day after submitting this review and offering a full refund.
Admittedly, I wrote this, feeling a bit frustrated that the product failed, but surprised with Anker's response with a request they be given another chance. Very commendable in today's world.
+1 star for their diligence.
Re:
Duly noted.
I'm sorry to have reported my experience with this product but it is the truth.
Thanks but no thanks for the refund offer. I hardwired a power cord to the charger and now it works without intermittent behavior thus sustaining its use.
Another chance? Of course. I like the product (and Anker) but do not like the micro USB connector it uses.
I may follow up with a secondary comment on the rapidity of your response.
This was a generalized comment for the industry to consider.
I only apologize that it was targeted solely at Anker.
You are not alone. Many of your competitor's products use the same connector so the consumer has little choice but to accept the limitations -or choose a product that does NOT use the mini or micro USB connector. Good luck with that.
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Reviewed in the United States on July 8, 2023
★★★★★ 5
Anker is the only option for your charging needs
Size: 1pack, Color: Black
The Anker 313 charging stand is a simple, reliable wireless charger that works great for my everyday use with my iPhone 16 Pro, especially for overnight charging. It’s a straightforward iPhone-compatible stand that may not be the best for speed, but this actually makes it ideal for bedside use.
One of the best features is that it charges through thicker cases. My phone has a fairly bulky case and it still charges without any issues. Its magnet isn't strong, but it will do the job. As I noted, charging speed is moderate rather than fast. So it won’t top up your battery quickly during the day, but that’s actually perfect for overnight use since it charges steadily without generating excessive heat concerns. The stand design is also convenient because you can keep the phone upright for notifications or Face ID, and it can even charge in landscape orientation if you want to watch videos while it’s docked.
Overall, the Anker 313 is a great budget-friendly wireless charger that does exactly what it needs to do. It is dependable charging, good case compatibility, and a stable stand design. It’s not meant to be the fastest charger, but for a nightstand setup with an iPhone 16 Pro, it’s a practical and reliable option. You can't go wrong with this one.
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Reviewed in the United States on March 6, 2026
★★★★★ 5
it works and it hasn’t failed me!
Size: 1pack, Color: Black
i’ve had this phone charger for a year and it works well! it charges decently and even flashes a little blue blinking light when it’s not placed on properly so you know if it’s charging or not. i will say the speed at which is charges is average, so it’s not a super fast charger.
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Reviewed in the United States on May 20, 2026
★★★★★ 5
Awesome product will not disappoint
Size: 1pack, Color: Black
This is something like the 5th+ one of these Anker 313 Wireless Charger Stands that I’ve purchased. Why 5+? Because these are really great wireless charging devices. I have bought myself a few, bought them for my sons and even, bought my ex-husband one. I’ve yet to have one not work, they just keep on providing a great charging system and having the cell phone sitting up like it does in the charger is a bonus. While cell is charging, if a call or text comes in, you can see that without picking up your cell. Also, you can be charging your device while you’re using it since this charging device props up your cell. On a side note, I did have a problem just recently with my cell not charging while on the stand, *BUT I found out it was the *charging cable that I had purchased separately that was defective. I replaced the cable with a new one and the Anker 313 Wireless Charger (Stand) was immediately charging again. If you are thinking about buying this charger, I recommend that you do. You will not be disappointed in this charger…
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Reviewed in the United States on April 22, 2026
★★★★★ 5
Good charger, great customer service
Size: 1pack, Color: Black
Charger is simple and works well. Stopped charging after a year, which is not very common for Anker, but I contacted support and they sent me a new one without a hassle, it has an 18 month warranty. I always liked this brand and this experience only reinforces their commitment to customer service.
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Reviewed in the United States on April 8, 2026