TGN46 Recombinant Rabbit mAb (S-3514-47)

TGN46 (Trans-Golgi Network protein 46 kDa) is a type I integral membrane protein predominantly localized to the trans-Golgi network (TGN), where it functions as a critical component of the protein sorting and vesicular trafficking machinery. This 46-kilodalton protein features a large N-terminal lumenal domain containing multiple N-glycosylation sites, a single transmembrane helix, and a short C-terminal cytoplasmic tail harboring sorting signals—including tyrosine-based motifs (YXXΦ)—that facilitate its interaction with adaptor protein complexes (AP-1 and AP-2) to regulate cargo selection and vesicle formation. TGN46 serves as a widely used marker for the TGN in immunofluorescence and cell biology studies, and plays essential roles in maintaining TGN structure, recycling mannose-6-phosphate receptors (MPRs) between endosomes and the Golgi, and sorting lysosomal enzymes into clathrin-coated vesicles destined for late endosomes. Its dynamic cycling between the TGN, endosomes, and the plasma membrane—mediated by phosphorylation-dependent conformational changes and interactions with various coat proteins—underscores its significance in secretory and endocytic pathway regulation, making it indispensable for proper cellular homeostasis and protein trafficking fidelity.