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Description
IdeS Protease (Tag Free)Product Specification Species Streptococcus pyogenes Synonyms IdeSIdeS ProteaseImmunoglubulin degrading enzyme Expression System E. coli Molecular Weight 38 kDa (Reducing) Purity 95% by SDS PAGE Conjugation Unconjugated Tag No Tag Physical Appearance Lyophilized Powder Storage Buffer PBS pH 6. 6 Stability & Storage 12 months from date of receipt, 20 to 70 C as supplied. It can be stored at 4 8 for one week after dissolution, under sterile conditions
Product Specification
| Species | Streptococcus pyogenes |
| Synonyms | IdeS、IdeS Protease、Immunoglubulin-degrading enzyme |
| Expression System | E.coli |
| Molecular Weight | 38 kDa (Reducing) |
| Purity | ＞95% by SDS-PAGE |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | PBS pH 6.6 |
| Stability & Storage |
·12 months from date of receipt, -20 to -70 °C as supplied. · It can be stored at 4-8℃ for one week after dissolution, under sterile conditions · Please avoid repeated freeze-thaw cycles. |
| Reference |
[2] Pawel-Rammingen V, U. IdeS, a novel streptococcal cysteine proteinase with unique specificity for immunoglobulin G[J]. Embo Journal, 2014, 21(7):1607-1615. |
Background
IdeS Protease is a cysteine hydrolase secreted
by the human pathogen Streptococcus pyogenes. This product use E. coli
recombinant expression, high purity, has good enzyme digestion activity, can
specifically identify IgG, and perform enzyme digestion at specific sites in
the hinge region of the antibody, and IgG can be hydrolyzed into F(ab')2
fragments and Fc fragments, which can identify human and other animal IgG, such
as mouse, rabbit, monkey, sheep and human animal chimeric IgG, etc, It can be
used for structural characterization analysis of antibodies and fusion protein
drugs
Components
Protocol
1. Add the desired amount of 5mg IgG in digestion buffer or other compatible
buffer * .
2. Add IdeS Protease(Tag Free) to the reaction system:
• Add 1 unit of IdeS Protease(Tag Free) per 1µg of IgG to be digested.
• For example, add 5µl (200 units) of reconstituted IdeS to digest 200µg of IgG.
3. Incubate sample at 37°C for 30–60 minutes
Unit Definition
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
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