Pay in installments of $187.50 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Aug 14 - Aug 19
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Organotal rat fetal normal small intestinal organoid culture KitProduct Specification Usage 1. Primary (1) the normal small intestine tissues of fetal rats after sampling must be cleaned at 2 8 , several Petri dishes should be prepared, and primary culture buffer B pre cooled at 4 should be added for standby (double antibody and gentamicin should be added) (2) put the tissue into the Petri dish, wash it three times with primary culture buffer B, remove impurities, remove the intestinal mucosa of fetal rat small
Product Specification
| Usage |
1. Primary (1) the normal small intestine tissues of fetal rats after sampling must be cleaned at 2-8 ℃, several Petri dishes should be prepared, and primary culture buffer B pre cooled at 4 ℃ should be added for standby (double antibody and gentamicin should be added) (2) put the tissue into the Petri dish, wash it three times with primary culture buffer B, remove impurities, remove the intestinal mucosa of fetal rat small intestine tissue with ophthalmic scissors or scalpel, and cut it into a tissue block with a volume of about 10-30mm3 (3) the tissue was digested with the digestive solution c of the primary tissue of the normal small intestine of rat fetus, with shaking digestion at 4 ℃ for 20-25min or standing digestion for 40-50min (observe the digestion at any time during the digestion process) (4) take a small amount of liquid and observe under the microscope. More crypts are observed under the microscope. Add three times the volume of primary culture buffer B to stop digestion (5) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation (6) Matrigel calculation: after step 5, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate (7) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃) (8) put the laid culture plate into a 37 ℃ incubator for 10-15min to form a glue, and add rat fetal rat normal small intestinal organoid medium a (restore room temperature) for culture 2. Organoid subculture (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4 b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4 (4) after organoid collection, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul of rat fetal normal small intestinal organoid culture medium A 3. Organoid cryopreservation (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid (4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube (5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage 4. Organoid resuscitation (1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube (2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting (3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min (4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g (5) resuspend the Matrigel, lay 25ul of Matrigel per well in a 24 well cell culture plate, place it in an incubator for 10-15min to form a gel, and add 500ul of rat fetal normal small intestinal organoid medium A. |
||||||||||||||||
| Synonym | Fetal Rat Small Intestine Organoid Culture Kit | ||||||||||||||||
| Description | Composition:
|
||||||||||||||||
| Storage Temp. | 4 ℃, 3months; -20 ℃, 1 year, see the label for details |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.1 ★★★★★
Based on 16 reviews
Sort
Product Reviews
★★★★★ 5
beginner friendly cord managing 10/10
Color: White, Size: 36"L x 5.5"W, Color: White, Size: 36"L x 5.5"W
Love how easy it was to set up and get to working on the new desk setup in my room. This made my setup 10x better and more efficient for me and my tiny fur buddies. Holds plenty of cords and is super easy to keep secured on the desk. 10/10 for me. I can tell it’ll last a while. I’m not a pro and the cable managing could be better but this helped a ton.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 17, 2026
★★★★★ 5
Worth the price!
Color: Black, Size: 36"L x 5.5"W
Great product and easy to install. I purchased the 36 inch version and it’s just the right size for my desk. Makes securing and hiding the wires a breeze.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 13, 2026
★★★★★ 5
The best way to manage your cables.
Color: White, Size: 36"L x 5.5"W
Best cable management product I have used. period. I have used hanging baskets, tried 3m taping channels and tucking everything away. While that works. I change my setup fairly regularly. So hours spent to keep the cables perfectly managed just have to be redone when you add something or take something away.
I love the design. The canvas has velcro stitched into it so you can strap heavier items like power strips and bricks into the canvas and then just run your cables through the channel. When you close the canvas all the cables are out of sight leaving something that looks clean but is also versatile enough to support and evolving setup.
After everything I have tried, this has been the best hands down.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 7, 2026
★★★★★ 5
Exactly what I needed!
Color: Black, Size: 28"L x 5.5"W
This cable management tray is exactly what I need to complete my new desk. Being able to keep my cables organized and off the floor is essential, especially with a standing desk. The installation process was very simple. The clamps do not take up much space on top of the desk, I barely notice them. Overall, the tray is very sturdy with a thick fabric. It holds a fairly large power strip well along with my other cables. I also like that I can just unhook one side when I need to access a cable.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 27, 2026
★★★★★ 5
Works well
Size: 6.5 FT, Color: Black
This thing was a life saver. It has plenty of plug ports for the price and it's not huge. The footprint is a bit bigger than some others but it's not bulky. Seems well built and the fact that I can power all my electronics and still have the USB ports to charge my phone and things is nice.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 22, 2026
recommand products
Sergio Lub - HORSEMAN Cuff Bracelet
75.00
King Engine Bearings Connecting Rod Bearing Set for 1987-1988 Toyota Corolla (CR4032SI0.25)
35.10
King Engine Bearings Connecting Rod Bearing Set for 1990-1992 Geo Prizm (CR4032SI)
33.00
King Engine Bearings Connecting Rod Bearing Set for 1995-1999 Toyota Tercel (CR4027AM1.0)
21.30
King Engine Bearings Connecting Rod Bearing Set (CR1018XA0.25)
20.16