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Description
Mouse GPX1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a glutathione peroxidase 1 (GPX1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of glutathione peroxidase 1 (GPX1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Glutathione Peroxidase 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Glutathione peroxidase 1, also known as GPX1, is an enzyme encoded by the GPX1 gene. This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase detoxifies hydrogen peroxide and is one of the most important antioxidant enzymes. It is characterized by a polymorphism in the N-terminal alanine sequence, including three alleles with five, six, or seven alanine (Ala) repeats. The allele with five Ala repeats is significantly associated with breast cancer risk. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.8 ★★★★★
Based on 20 reviews
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Product Reviews
★★★★★ 5
Very nicely made and easily filled for use.
Color: Silver single
Perfect for our kitchen. The ideal sea salt dispenser....and it is fully adjustable from coarse to fine.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 10, 2026
★★★★★ 5
Great quality
Color: Silver single
Just what I was looking for in a salt shaker. It’s on the smaller side and the quality is great. I’m very satisfied with my purchase.
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Reviewed in the United States on May 7, 2026
★★★★★ 5
Great size, design, and 100% metal grinding mechanism
Color: 1pcs-Sliver-304
***PLEASE NOTE: This review is only for the more expensive version here with the all metal grinding mechanism and not the cheaper version that looks the same but has a ceramic grinder that’s also available on this page — hope this helps avoid potential confusion. My advice is to splurge on the metal grinder version.
***UPDATE 3/18/26***
Well, after almost two years of fairly heavy daily use the first flaw has emerged so thought I’d share. Yesterday the silver wrapping on the grinder part came loose making it hard to turn the grinder as it was slipping. I did a quick fix by adding some super glue around the seam at the top of the grinder and that’s done the trick at least for now. I’m leaving my 5-star rating because I remain thrilled with its overall performance and frankly would’ve ordered another one if this fix didn’t work, but so far it appears to be holding. 🤞 So still very happy but just wanted to share in case anyone else experiences this.
***UPDATE 8/18/25***
So I’ve now had this for over a year, and under pretty heavy daily use I’m happy to report it still works as well as the day I bought it. Great product, and it’s a pleasure to use every day.
***UPDATE 10/3/2024***
So, after almost four months of pretty heavy daily use I just wanted to report back that this grinder continues to work perfectly and shows every sign of lasting for years to come. I especially like the glass jar so I can see when I’m running low on black peppercorns, and even though it’s got a small form factor it holds a lot so I don’t have to refill it frequently despite using it multiple times daily. I’m super happy with this product all around and recommend it highly!!!
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Been using this for a few days now and am very happy so far. I’ve used a traditional wood pepper mill my whole life, but now it’s just me so I wanted something smaller and with a glass body so I can easily see when I need to add more peppercorns. I also wanted an all metal grinding mechanism (that this version has for an few dollars more) instead of ceramic, which doesn’t seem to hold up as well, and I definitely wanted no plastic parts involved in the actual grinding either — don’t need any more microplastics in my diet thank you. This one seemed to check all the boxes, and so far it’s been great. Initially it was set to a more fine, powdery grind, but it was super easy to adjust to a more course grind that I prefer and it’s perfect. Overall it seems well built and is very easy to use, and as long as this holds up it’s a definite keeper. I’ll report back if any future mishaps, but as of now I’d recommend this very highly!
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Reviewed in the United States on June 21, 2024
★★★★★ 5
Most excellent spice grinder!
Color: 1pcs-Sliver-304
This spice grinder grinds my cumin and coriander seeds perfectly with very little effort and it’s attractive. I’m sure it will last longer than my plastic one that broke recently. Very happy with my purchase!
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Reviewed in the United States on May 31, 2026
★★★★★ 5
Quality is good considering low cost.
Color: Silver1
Good quality for an inexpensive salt/pepper grinder. Works fine and should last a reasonable time
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Reviewed in the United States on May 28, 2026