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Description
Human PDZK1IP1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment:
1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against PDZK1-interacting protein 1 (PDZK1IP1). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of PDZK1-interacting protein 1 (PDZK1IP1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human PDZK1-interacting protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | PDZK1-interacting protein 1 (MAP17) is a protein encoded by the PDZK1IP1 gene. It has been shown to interact with PDZK1. Diseases associated with PDZK1IP1 include enamel caries and spinal muscular atrophy type IV. An important homologue of this gene is SMIM24. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.7 ★★★★★
Based on 18 reviews
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Product Reviews
★★★★★ 5
It brightens before your eyes!
You know how some products need time before you see the difference, this one doesn’t. As soon as you apply it you can visibly see how light your under eye is. The formula is very rich, not too moist it just makes my under eyes look sooo vibrant!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 30, 2024
★★★★★ 5
52 with Mature Skin — Hydrating, Smoothing Eye Patches That Actually Make a Visible Difference
Color: Pink (PDRN), Color: Pink (PDRN)
I’m 52, post-menopausal, with drier, more delicate skin around the eyes, and I’ve been using Abib products for years. It’s one of those brands I consistently come back to because the formulas are well-balanced and actually perform without irritating my skin. These patches fit right in with that experience.
The texture is one of the first things that stands out. They have a cushiony, jelly-like feel that holds its shape well and adheres comfortably to the skin. Once applied, they stay in place without sliding around, and there’s no stickiness or residue left behind when you remove them — which is not always the case with eye patches.
In terms of effectiveness, these go beyond just a temporary cooling effect. With consistent use, I notice my under-eye area looks smoother, more hydrated, and less tired overall. Fine lines look softened, and the skin has a more refined, even appearance. Puffiness is reduced, and the area just looks more awake and supported.
Hydration and softness are where these really shine. After using them, the skin feels noticeably softer and more comfortable, not tight or overly treated. There’s a real improvement in how the skin holds moisture, which is especially important for mature skin. That added hydration translates directly into a smoother, more polished look.
I also use these beyond just the under-eye area. I’ve been applying them to my nasolabial folds, and they work beautifully there as well. That area tends to show dryness and creasing, and these patches help soften and plump the look of the skin, making everything appear more relaxed and less etched.
The finish they leave behind has a subtle glow — not shiny or greasy, but a healthy, hydrated look that makes the skin appear fresher and more even. It’s the kind of glow that comes from improved skin condition, not just surface-level shine.
From a value standpoint, the jar contains a generous number of patches, and because they can be used in multiple areas, they feel much more worthwhile than single-use treatments. A little time with these gives visible results, which makes them a good addition to a routine.
Overall, these are very well done. They deliver on hydration, softness, and smoothing while remaining gentle and comfortable to use. If you’re dealing with dryness, fine lines, or puffiness — especially with mature skin — these are absolutely worth trying.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 20, 2026
★★★★★ 5
Great product
Color: Pink (PDRN)
These eye patches are incredibly hydrating and leave my under eye area looking plumper and refreshed after using. Adhere well without slipping and deliver an immediate cooling, refreshed effect. The formula feels gentle yet effective, and the patches are generously sized and comfortable. I purchased these during a promotional deal and felt they were an excellent value for the quality. A high-quality product that fits seamlessly into a skincare routine.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 26, 2026
★★★★★ 5
Gentle Retinal — No Irritation for Me
Color: Pink (PDRN)
I was honestly nervous to try these because they contain retinal, and the under-eye area can be sensitive. But I had zero irritation. No burning, no redness, no peeling.
They feel cooling and soothing when applied, and the hydrogel texture stays in place without sliding around.
After consistent use, my under-eye area looks smoother and more refreshed. I’ve noticed subtle improvement in fine lines and overall brightness.
If you’re curious about trying retinal in a gentle way, this is a great entry point. Just make sure to use sunscreen during the day, as you would with any vitamin A product.
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Reviewed in the United States on February 28, 2026
★★★★★ 4
Hydrates well
Color: Pink (PDRN)
So far the product has been nice. My understanding is that eye area looks hydrated. It does not diminish the under eye bags. Have been using for a few weeks. Maybe I’ll give it more time to see better results.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 1, 2026
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