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Description
Histone H3 (acetyl K9) Rabbit polyclonal antibodyProduct Specification Host Rabbit Synonyms H3K9ac Immunogen Synthetic Peptide Location Nucleus Accession P68431 Antibody Type Polyclonal antibody Isotype IgG Application WB, IHC P, ICC, ChIP Reactivity Hu, Ms, Rt Predicted Reactivity Fu, Pl, Ys, SeUr, Dr, C. el, Hm, Or, Bv, Xe Purification Protein A Concentration 0. 5 mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS, 40% Glycerol, 0. 05%BSA, 0. 03% Proclin 300 Stability &
Product Specification
| Host | Rabbit |
| Synonyms | H3K9ac |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P68431 |
| Antibody Type | Polyclonal antibody |
| Isotype | IgG |
| Application | WB, IHC-P, ICC, ChIP |
| Reactivity | Hu, Ms, Rt |
| Predicted Reactivity | Fu, Pl, Ys, SeUr, Dr, C.el, Hm, Or, Bv, Xe |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| Dot Blot | 1:1000 | |
| WB | 1:1000 | |
| IHC-P | 1:500 | |
| ICC | 1:500 | |
| ChIP | 1:20~1:50 |
Background
H3K9ac is an epigenetic modification to the DNA packaging protein Histone H3. It is a mark that indicates the acetylation at the 9th lysine residue of the histone H3 protein. The H3K9 histone has two jobs. Genes get turned on if this mark is acetylated and silences them if methylated. H3K9ac is an important acetylation and connected with active promoters. H3K9ac and H3K14ac have been shown to be part of the active promoter state. They are also present over bivalent promoters and active enhancers. This is also a mark for liver cancer through a defect in the H3K9ac/H3K9me3 transition.
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