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Description
Human Lysozyme OneStep ELISA kitsProduct Specification Antigen Lysozyme Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Hu Purification Protein A Stability & Storage 12 months from date of receipt reconstitution, 2 to 8'C as supplied. Kit Precision Intra assay: 4. 3% Inter assay: 6. 4% Sample type Cell culture supernatant, Serum,Plasma Assay type Sandwich (quantitative) Sensitivity 2. 167 pg mL Range 15. 63 pg mL 1000 pg mL Recovery Cell culture supernatant:
Product Specification
| Antigen | Lysozyme |
| Immunogen | Recombinant Protein |
| Antibody Type | Recombinant mAb |
| Reactivity | Hu |
| Purification | Protein A |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8'C as supplied. |
Kit
| Precision | Intra-assay: 4.3%； Inter-assay: 6.4% |
| Sample type | Cell culture supernatant, Serum,Plasma |
| Assay type | Sandwich (quantitative) |
| Sensitivity | 2.167 pg/mL |
| Range | 15.63 pg/mL – 1000 pg/mL |
| Recovery | Cell culture supernatant: 87% Serum: 86% Plasma: 102% |
| Assay time | 60 minutes |
| Species reactivity | Human |
Background
Human lysozyme (LZM/LYZ), a 14.4-kDa bacteriolytic enzyme encoded by the LYZ gene on chromosome 12, functions as a critical innate immune defender by hydrolyzing β-1,4-glycosidic bonds in peptidoglycan of bacterial cell walls. Primarily secreted by neutrophils, macrophages, and epithelial cells, it is abundantly present in secretions (tears, saliva) and biological fluids, with serum/plasma concentrations typically ranging from 1.5–3.0 μg/mL in healthy adults. Clinically, elevated serum levels (>5 μg/mL) serve as a biomarker for bacterial infections (e.g., tuberculosis, meningitis), monocytic leukemia (due to malignant cell release), and renal tubular dysfunction (reduced reabsorption), while decreased levels correlate with immunosuppression. Detection relies on high-sensitivity ELISA (limit: ~0.5 ng/mL), though sample handling requires rigorous centrifugation and -80°C storage to prevent degradation. Its dual role as an antimicrobial agent and disease indicator underscores its significance in both physiological immunity and pathological diagnostics.
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