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Description
Mouse TBK1 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a TANK Binding Kinase 1 (TBK1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of TANK Binding Kinase 1 (TBK1) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse TANK Binding Kinase 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | TANK-binding kinase 1, also known as TBK1, is an enzyme encoded by the TBK1 gene. It is an enzyme with kinase activity. Specifically, it is a serine/threonine protein kinase. This kinase is primarily known for its role in the innate immune antiviral response. However, it also regulates cell proliferation, apoptosis, autophagy, and anti-tumor immunity. Deregulation of its activity can lead to autoimmunity, neurodegenerative diseases, or tumorigenesis. It is a non-canonical IKK kinase that phosphorylates nuclear factor kappa B (NFkB). It shares sequence homology with canonical IKK. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.7 ★★★★★
Based on 10 reviews
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Product Reviews
★★★★★ 5
Amazing buds/price/service... buy these!
Style: Standard, Color: Purple
Yes, my subject line sounds like that trash hyperbole you get on annoying pop-up ads on your Smartphone! And ironically, it usually has to do with ear buds. There are tons of them on the market, and everyone claims to be the best.
Now, first off, I am just a customer… never got any gifts or money or anything from this company, nor was asked to “click on a review” for a discount or whatever. No… the reason for all this hallelujah chorus, is that I’m really totally both jazzed, and a bit in awe of these earbuds. I think the main mind-blowing thing about them is the price. I mean, I have a bit of experience and knowledge with this kind of technology, and frankly, these days, what “guts” that are needed to be used to create Bluetooth ear buds is not that complex… nor expensive. How company’s like Sony, Apple, Bose, etc. can charge upwards of $300 for these boarders on theft and fraud!
I’ve tried those “Rolls Royce” buds, and I urge you to do the same, and then try these Tagry Bluetooth Wireless Earbuds that are literally 90% less expensive. Yes, they retail for about $35! They come in an excellent quality charge base, are extremely easy to put on, (and have three extra size buds to give you a custom fit), and most important, their sound quality, and I mean crisp highs, solid middles, and bone- rumbling base, are outstanding. I cannot understand what more you would want either using them for talk radio, podcasts, YouTube, streaming movies, or TV, or playing your Amazon Music or Spotify or iTunes playlists. And yes, if connected to your iPhone, you can make and receive calls, and the microphone in the buds are top quality.
Their Bluetooth software is flawless, connecting to your iPhone, or other Bluetooth devices effortlessly. They come with a pleasant woman’s voice letting you know various messages, like which earbud is in what ear, if they are connected or turned off, your volume control (including excess volume warning), etc.
I’ve had these for several months, and have found the charging, which has a bright LED display and shows the individual charging of each bud, along with their percentage easy to read.
And a note on their service. I’ve heard horror stories of folks trying to reach the outrageously over-priced other earbud company’s for tech support, or refunds, exchanges, etc. Basically it’s nonexistent, and when you do miraculously find a phone number to call them, and get someone on the line, you have to go to the UN to find an interpreter of rare third-world languages to understand what they are saying, and they seem to be trained, not on anything to do with the product, but to make the call as useless and confusing as possible, so that you will just give up and throw their buds, and your money in the trash.
Now, I did have an initial problem, after a few months, in that the base charger seemed to be slowing a bit. Tagry gives you a LIFETIME WARRANTY… yes, an actually lifetime warranty, not one of those sham “lifetime warranties”, that corrupt company’s like Hammacher Schlemmer have.
I had enrolled with Tagry, on their website, to activate my warranty as soon as I received the buds. I called Tagry when I was seeing what could be a glitch in their charging base, and yes, unlike most other companies who hide their contact numbers as if they were nuclear codes, Tagry’s support phone number is plainly printed on their manual, and to my astonishment, I immediately was connected to a good ‘ol American customer service person, who actually worked for the company, spoke English, and made me feel as if I was a long-ago trusted friend. They truly cared, and said they were sorry, and that there was no need to troubleshoot the base, or return it, instead, they would process an order to send me a new one.
A day later, they actually called me back to let me know that the replacement had been shipped, and that I should have it in a day or so, and if not, to please call them back right away. And yes, to apologize again for any inconvenience. The replacement arrived the very next day. And again, their warranty is indeed for a lifetime, a no questions asked replacement policy. This is how much they believe in their product, and respect their customers.
So, yes. It is insane to pay more than $35 for quality Bluetooth earbuds. Last year, I sent about a dozen of these out to friends and employees, to use for personal use, and for all that Zooming going on… and they love them. And none of had any problems.
I’m sure you have had a plethora of bad experiences in the past few years, what with isolation, and way too much ordering on-line, finding the products you buy to be substandard, and customer service virtually non-existent. And that is why I’ve spend all this time expounding on the greatest of my earbuds. Yes, I have a life. Really. But what a joy to be able to tell a story like this, rather than the kind of frustration most of us are experiencing every day!
Yes, goodness and quality still exist. It’s rare… but it’s out there!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 24, 2023
★★★★★ 4
A big bang for your buck!
Style: Standard, Color: Pink, Style: Standard, Color: Pink
I waited a while to review. I wanted to see how they did overtime. These are amazing for the price. The sound is crisp and clear and even can go extremely loud if that's your thing. They fit comfortably in my ear and are durable (I've dropped them a few times). The case holds a charge for a long time they connect to Bluetooth with no problems. I only gave 4 stars because there is a slight delay when you tap the ear buds to change functions. It's also a small spot to touch and sometimes I find myself having to tap it a few times to find the right spot to turn on/off, turn volume up, change songs, etc. These are simple with great sound and an amazing price. If you're a sound connoisseur and want a equalizer board to work with these aren't for you. I have expensive Jabra ear buds and find I use these more. They fit better and I don't worry about losing them.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 16, 2026
★★★★★ 5
Great quality
Style: Standard, Color: Forest Green
Very surprised at the quality of these headphones. They’re comfortable in the ear and have very clear sound quality and great volume. They’re easy to set up and the ear bud is just the right size to sit comfortably. Settings are easy to adjust and the battery life is great. I will say the color doesn’t match to the display image, they’re a lot darker green, but that’s not really an issue. I just wanted to get a colorful pair to make them distinct from others. These are a great budget ear bud!!
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Reviewed in the United States on May 6, 2026
★★★★★ 5
These Budget Earbuds Actually Impressed My Wife (and Our Wallet)
Style: Standard, Color: Ocean Blue
We’re retired and try to be smart with money, so when my wife wanted a pair of wireless earbuds for her daily walks and audiobook listening, I wasn’t about to drop $150+ on a big name brand. After researching, I took a chance on these TAGRY earbuds and I’m honestly glad I did.
She’s been using them almost every day for a few months now, and they still work great. The battery life is legitimately impressive — the case shows the charge level with a little LED display, which is surprisingly handy, and we easily get several days of use before needing to recharge. The wireless charging is convenient too. Sound quality is clear and perfectly decent for the price — good enough for her favorite music, podcasts, and even taking phone calls in the house. They stay in her ears well during walks, and the IPX5 waterproof rating gives us peace of mind when it’s drizzly out.
The only small complaint is that they’re not quite as comfortable for really long wear (she sometimes switches ears after a couple hours), and the bass isn’t super deep compared to premium models. But for everyday use, that’s a very minor gripe.
Overall, these have been a solid, no-fuss purchase that delivers way more than we expected for the money. If you’re a senior, on a budget, or just don’t want to overpay for earbuds, these are worth trying.
Hope this helps someone!
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Reviewed in the United States on May 25, 2026
★★★★★ 5
Really great pair of headphones
Style: Standard, Color: Black
These headphones work really well. They charge up quickly and the battery lasts a long time. These are way better than my old pair that I replaced with these. Great price.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 23, 2026
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