Pay in installments of $46.03 with
,
and
Shipping Estimate
USA
- USA
- CAN
- USA
- CAN
Ships within 48 hours · Estimated delivery Jul 23 - Jul 28
Promo Codes Available:
For Your Every Summer RSVP, with Code: SUMMER15
Description
Human FBN1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
|||||||||||||||||||||||||||||||||
| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a fibrillin 1 (FBN1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the fibrillin 1 (FBN1) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Fibrillin 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
|
|||||||||||||||||||||||||||||||||
| Background | Fibrillin-1 (FBN1) is a protein encoded by the FBN1 gene located on chromosome 15. It is a 230 kb gene with 65 coding exons that encodes a 2,871-amino acid proprotein named profibrillin. This proprotein is cleaved near its C-terminus by the furanyl invertase protein to yield fibrillin-1, a member of the fibrin family, and asprosin, a 140-amino acid protein hormone. It is a large extracellular matrix glycoprotein that serves as a structural component of 10-12 nanometer calcium-binding microfibrils. These microfibrils provide force-bearing structural support in elastic and inelastic connective tissues throughout the body. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
|||||||||||||||||||||||||||||||||
| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy
4.1 ★★★★★
Based on 10 reviews
Sort
Product Reviews
★★★★★ 5
Wonderful chair
Color: Dark Black
I bought this office chair as a sewing tool, you might say. I felt that having an office chair on wheels would get me from the sewing machine to the ironing board and then to the cutting board and back to the sewing machine. I was having to get up and down to get around to all these places before and this saves me so much time and effort.
I’m 71 so when the box came I was hesitant to try to bust into it and put it together but it went together so easily. It’s very comfortable and the lumbar support feels great on my low back. When I first sat down after the assembly process, the chair cushion tipped down in the front but after contacting the support team I was advised to tighten the tilt tension located under the chair and that fixed me right up!
All and all, my chair is the perfect addition to my sewing area at a very affordable price. I couldn’t be happier!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 31, 2024
★★★★★ 4
Comfortable, affordable, and great for small spaces!
Color: Light Purple, Color: Light Purple
I had previously left a review of two stars, but have since deleted that review after I've owned the chair for a few weeks now. Initially, I stated the chair was uncomfortable and not cushioned at all. But this issue quickly resolved after sitting it for a longer time, it seems the cushion molds to you. So it may be uncomfortable the first few times sitting on it, but it eventually softens. The cushion is now soft yet sturdy. You don't sink in too much, but it's comfortable for sitting for long periods of time. I personally enjoy the mesh back as well, it supports my lower back and doesn't cause back pain. Normally I have to use a cushion of my own to support my lower back for most chairs, but this one actually supports me in a way that doesn't require an extra cushion - so that's really nice.
The chair is fairly on the smaller end, which is perfect for low desks or tight spaces, which is exactly what I wanted. The height can be adjusted, but even at the highest setting, it is still fairly low. Keep that in mind - it may not be super comfortable for those over 6 ft. The arms flip up which makes storing it under a low desk very easy. The cushion is thick and very accurate to the picture. I love that it is lightweight and doesn't make much noise when rolling the chair. It also feels very sturdy. Some of the reviews say it feels flimsy, and I don't know if some are just assembling it incorrectly, because for me this thing is very strong and holds up quite nicely under my weight.
Overall, this is exactly what I wanted for my personal living space. A chair that's not too bulky, but comfortable enough to sit in for long hours. It functions as advertised for this purpose.
One final thing -- the color selection is gorgeous. I bought the light purple color and it fits my setup nicely! Color was accurate to the picture, and looks very cute and adorable in my space.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on December 21, 2025
★★★★★ 5
Exteremly comfortable
Color: Dark Black
Reasonably priced, very comfortable l, easy assembly
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 24, 2026
★★★★★ 5
Surprisingly amazing chair!
Color: Pink, Color: Pink
tl;dr: If you need a chair that has the features of this one, then this one is a winner! Hits above its weight class for sure.
My wife is 5’. She’s small. For whatever reason she ended up with a chair made for big people, like 6+ feet and up to 500lbs type of a chair. She’d often even sleep on it and chill on it. The chair was big enough to fit 2 of her to be honest. It was an expensive chair that I won at some raffle and didn’t like myself. Over the years it ripped (got a cover for it) and even started to get lopsided (from the sleeping) and finally a spring broke (hence the pillow on it in the pictures)
She obviously lives pink. I also wanted to get a chair where she could work and not end ip asleep on. Not like we have a couch or a bed for that 😂.
This chair is very obviously smaller than her previous chair, it’s also significantly cheaper in price and build, it’s also not as durable feeling or stable, and it’s just not as padded. Yet somehow it’s an amazing chair that we both like better than her previous chair???
Like it’s crazy that this chair somehow works better in so many ways. Her desk is modified to be shorter than normal desks (again, she small lol) and her previous chair was just too big. The wheels are also like SUPER smooth, like you can run a chair race easily with it. It rolls over her rug and tucks into her desk (which is in our bedroom) easily. The amount of space this has save us when the chair isn’t being used is a lot. Previous chair was much bigger and didn’t tuck in at all.
The color is nice and the padding is plenty for a small and much lighter than 500lbs woman. I’m 6 feet and 200 lbs and the chair is nice for me as well. My chair is bigger and more secure in the backing, which is similar to this one. The lowest tension setting on my chair is still too high for her to lean back on my chair. This chair has a lower tension minimum and she can easily lean back freely, as well as keep enough tension to work without sagging.
This chair is surprisingly outstanding! I’m sure my wife will destroy it quicker than the last one, but it’s so cheap that even if it only lasts 1-3 years, I’ll happily buy it ones or twice more 🤷🏽♀️. Maybe by then they’ll invent a comfortable titanium chair that’s affordable that she won’t destroy 😂.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 28, 2024
★★★★★ 3
Mid.
Color: Light Purple
The chair is fine, and reasonably easy to put together. However, I've been using it for a little over a year and a half, and it's already falling apart. Random parts of it have fallen off, which is fine because it was still functional. But now the most frustrating part is that the wheels continue to pop off, and while the first attempts to pop them back on worked, they will not stay on now. This is obviously greatly impacting the stability, and I will need to purchase a replacement soon. For this price and lack of long-term durability, I would not buy again.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 4, 2026