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Description
LIF Protein, MouseProduct Specification Species Mouse Synonyms LIF, Leukemia Inhibitory Factor, HILDA, MLPLI, CDF, DIA Accession P09056 Amino Acid Sequence Ser24 Phe203 Expression System E. coli Molecular Weight 20kDa Purity >95% by SDS PAGE Endotoxin <1EU g Conjugation Unconjugated Tag No Tag Physical Appearance Lyophilized Powder Storage Buffer 20mM Tris, 200mM NaCl, pH8. 0 Reconstitution Reconstitute at 0. 1 1 mg ml according to the size in ultrapure water after
Product Specification
| Species | Mouse |
| Synonyms | LIF, Leukemia Inhibitory Factor, HILDA, MLPLI, CDF, DIA |
| Accession | P09056 |
| Amino Acid Sequence | Ser24-Phe203 |
| Expression System | E.coli |
| Molecular Weight | 20kDa |
| Purity | >95% by SDS-PAGE |
| Endotoxin | <1EU/μg |
| Conjugation | Unconjugated |
| Tag | No Tag |
| Physical Appearance | Lyophilized Powder |
| Storage Buffer | 20mM Tris, 200mM NaCl, pH8.0 |
| Reconstitution | Reconstitute at 0.1-1 mg/ml according to the size in ultrapure water after rapid centrifugation. |
| Stability & Storage | · 12 months from date of receipt, lyophilized powder stored at -20 to -80℃. · 3 months, -20 to -80℃ under sterile conditions after reconstitution. · 1 week, 2 to 8℃ under sterile conditions after reconstitution. · Please avoid repeated freeze-thaw cycles. |
| Reference | 1、Ryffel B. et al. (1993) Pathology induced by leukemia inhibitory factor. International Review of Experimental Pathology. 34: 69-72. |
Background
The leukemia inhibitory factor (LIF) belongs to the interleukin-6 (IL-6) cytokine family, which is involved in various biological processes including inflammation, immune responses, and embryonic development. Leukemia inhibitory factor is a glycoprotein growth and differentiation factor with pleiotropic activity. LIF has potent effects on the hematopoietic system, including megakaryocyte progenitor cells. In addition, LIF has bone regeneration activity, induces cachexia and acute-phase response in hepatocytes, and inhibits adipogenesis, to mention the more important activities. In vivo LIF treatment in monkeys and rodents was followed by signs of general toxicity, cachexia, acute-phase reaction, and stimulation of hematopoiesis. The safety margin for possible therapeutic effects on hematopoiesis seems to be very narrow.
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