Human IgM ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA detection technology. Specific anti human IgM capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the microplate plate for incubation. After incubation, the IgM present in the sample binds to the solid-phase antibody. After washing to remove unbound material, horseradish peroxidase (HRP) - labeled detection antibody was added for incubation. After washing, the chromogenic substrate TMB was added to avoid light for color development. The depth of color reaction is directly proportional to the concentration of IgM in the sample. The reaction was stopped by adding stop solution, and the absorbance value was measured at 450 nm wavelength (correction wavelength 570 - 630 nm).

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, HRP labeled IgM detection antibody, detection buffer, TMB chromogenic substrate, washing solution, termination solution, plate sealing membrane and instructions.

Sensitivity: 1.37 ng/ml

Detection Range: 15.63-1000 ng/ml

Recovery Range: 92-110%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Immunoglobulin M (IgM)It is produced by B cells and is by far the largest antibody in the human circulatory system. IgM is the first antibody produced by the body after the initial stimulation of antigen. The plasmablasts in the spleen produce antibodies, and the spleen is also the main site of specific IgM production. IgM mainly exists in serum, because of the presence of J chain, it is also an important secreted immunoglobulin. IgM appeared at the initial stage of infection, and the amount of IgM produced during reinfection was small. IgM antibodies cannot pass through the human placenta. These two biological properties of IgM make it very useful for the diagnosis of infectious diseases. The presence of IgM in the patient's serum indicates recent infection, and the presence of IgM in the newborn's serum indicates intrauterine infection.