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Description
Human™ ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge at 1000g for 15 minutes at 2 8C within 30 minutes of collection. Remove the supernatant for testing or store at
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample handling and requirements: Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge at 1000×g for 15 minutes at 2-8°C within 30 minutes of collection. Remove the supernatant for testing or store at -20°C or -80°C, but avoid repeated freezing and thawing. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a thrombomodulin (TM) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and then to yellow by acid. The intensity of the color is positively correlated with the amount of thrombomodulin (TM) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Thrombomodulin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Thrombomodulin (TM) is an integral membrane protein expressed on the surface of endothelial cells that acts as a cofactor for thrombin. It reduces blood coagulation by converting thrombin from a prothrombin to an antithrombin. TM is also expressed in human mesothelial cells, monocytes, and subsets of dendritic cells. In humans, thrombomodulin is encoded by the THBD gene. The protein has a molecular mass of 74 kDa and consists of a single chain with six tandem repeats of an EGF-like structure, a serine/threonine-rich spacer, and a transmembrane domain. Thrombomodulin is also a glycoprotein on the surface of endothelial cells that, in addition to binding thrombin, regulates the inactivation of C3b by factor I. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | plasma |
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4.5 ★★★★★
Based on 24 reviews
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Product Reviews
★★★★★ 5
Eradicated Decade Long battle with KP on INNER KNEES in less than 1 week. NEW KP HOLY GRAIL CREAM!!
Scent: Sweet Vanilla & Soft Wood, Size: 10 Fl Oz (Pack of 1)
About my skin- 43 year old fair, normal to dry, very sensitive, prone to KP bumps on back of arms, inner knees and if aggravated - all over thighs. I am pretty careful with what I use and I even have to be careful within the same brand if something DOES work. I have spent a long time being snobby about “drugstore” brands because over the years they have all aggravated my now mostly under control KP. I recently was convinced to try the Olay SUper Serum body wash because a friend with similar issues said she was really impressed. I have been also! In fact, the body wash for “Rough and Bumpy” skin has been my go-to wash for the past few months and has visibly improved my overall skin texture and has not aggravated any KP flare ups. I was very excited to try this body cream, since the current one I use is over 50 dollars for a similar size jar. I like to use A LOT of moisturizer and the cost is really annoying and close to being prohibitive when you use a jar a week. I purchased this cream (packaged well, no leaked, fresh product, overnight delivery). I am in LOVE! It smells great, very high end. The fragrance is real so if you hate fragrance, bot for you. I wear Baccarat Rouge and this lotion does not interfere with that, and it does dissipate after a while (I asked others to be sure). The crazy thing is this… most of the time my KP bumps on my inner knees are there, but when I have them under control they just lack color and are a lot smoother. THEY ARE GONE AFTER 1 WEEK.. THIS IS THE FIRST TIME IN THE DECADE OR SO I HAVE HAD KP BUMPS ON MY INNER KNEEES THAT THEY HAVE BEEN COMPLETELY GONE. I do not feel it is the combo of the wash and this cream, as I have been using a different wash while out of town. The only new item I used was THIS CREAM, alone, without my other more expensive product. I am kind of blown away and delighted. Also, I was able to use less of this to feel moisturized than I have been using with the “fancy” brand. The scent is warm, slightly vanilla, expensive and not too floral. Cream absorbs quickly and well, leaves skin feeling plumped, soft, no greasy residue and MAJOR BONUS… NO PILLING. That is a nightmare when a product does that. It absorbs quickly, leaves no marks on clothes when squeezing into a pair of black legggings…. All the good stuff you may forget to look for but notice when they happen. I HIGHLY RECOMMEND TRYING, if a light, high end fragrance is good with you. To reiterate- the fragrance is not crazy strong but it also calms WAY down about 30 mins after applying and melts into just a nice feminine clean but warm (not body spray type of vibe) scent that is not very noticeable to me or others. No where near as strong as like a Bum Bum cream (which I LOVE IN SUMMER) and smells high end, not like cheap shampoo or air freshener. Get this!!!!!!! It also did not irritate my chest area which for me is always a little prone to red spots when I use fragranced products. No irritation and dude… NO BUMPS FOR FIRST TIME IN A DECADE OR MORE. WOW!!!!
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Reviewed in the United States on October 9, 2025
★★★★★ 5
THE BEST LOTION I HAVE EVER USED
Scent: Sweet Vanilla & Soft Wood, Size: 18.5 Fl Oz (Pack of 1)
THIS WINTER AND HAVING THE HEAT ON HIGHER THEN I NORMALLY DO HAS DONE A NUMBER ON MY SKIN. IT IS SO DRY AND I HAVE TRIED SO MANY THINGS AND NOTHING HAS REALLY WORKED. SO I GAVE IN AND SPENT THE MONEY AND TRIED THIS. I AM SO GLAD I DID. THIS WAS WORTH EVERY PENNY. I LOVED IT SO MUCH I BOUGHT ANOTHER BOTTLE JUST TO HAVE SO I WOULDN'T RUN OUT. I START OUT WITH THE BODY WASH WITH THE OLAY SUPER SERUM BODY WASH AND JUST AFTER ONE WASH MY SKIN WAS MUCH SOFTER AND SMOOTHER. YOU DO NOT NEED A LOT AS IT LATHERS UP NICELY IT SMELLS GREAT AND IT RINSES OFF GREAT LEAVING YOUR SKIN FEELING CLEAN AND SOFT. IT DOESN'T HAVE A STRONG SCENT TO IT WHICH IS FINE . I FOLLOW IT WITH IT WITH THIS SUPER SERUM LOTION JUST TO MAKE SURE THE SKIN STAYS SOFT. THIS LOTION IS SO SOFT AND SILKY. IT ABSORBS QUICKLY INTO YOUR SKIN WITHOUT LEAVING YOUR SKIN FEELING GREASY. IT IS LIGHTWEIGHT AND LEAVES YOU FEELING SOFT WITHOUT THAT DRY CRACKED APPEARANCE. THE SCENT ISN'T VERY STRONG TO ME WHICH IS FINE BECAUSE I WEAR PERFUME SO I DO NOT HAVE TO WORRY ABOUT THE SCENTS MIXING. I DEFINITELY RECOMMEND THIS REGIMEN TO ANYONE SUFFERING FROM DRY SKIN.
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Reviewed in the United States on March 27, 2026
★★★★★ 4
4.5/5; Great product, but smells strong
Scent: Sweet Vanilla & Soft Wood, Size: 10 Fl Oz (Pack of 1)
Context:
To start off, I should say that I have dry skin. Like, VERY dry skin. In purchasing this item, I wanted a product that I could consistently apply every single day to hydrate my skin.
Product:
The application of this stuff was like a dream. If you’ve ever tried using ANY sort of lotion (Lubriderm, Cetaphil, etc.) you’d know that usually it leaves that greasy texture on your hands that you have to wash off. This one doesn’t do ANY of that. The cream melts into your skin and absorbs very easily, unlike other lotions. I didn’t need that much to apply to my entire body. The results were great—my skin looked better and felt softer. If there was to be a con, it would be how strong the fragrance of the lotion is. This stuff smells STRONG. It’s not an unpleasant scent, but if you wanted to wear a perfume or body spray along with it, good luck because I can’t see a world where the cream won’t overpower or at least show through your preferred scent.
TLDR
Overall, good product with dreamlike application, but strength of scent could be dealbreaker for some.
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Reviewed in the United States on April 26, 2026
★★★★★ 5
This everyday body lotion is the bomb!
Scent: Collagen, Size: 17 Fl Oz (Pack of 1)
This body lotion is absolutely fabulous! It really does make the skin on my thighs and abdomen look and feel firmer and the skin on my arms, legs, hands, and feet is noticeably softer and more hydrated without looking greasy or feeling sticky. I even use it on my neck and my face, which has tended to be more dry than oily as I've aged. I also really appreciate that the scent is pleasant enough to stand on its own but subtle enough to work well with other fragrances. I don't think that I have ever been this satisfied with a health/beauty product at such an affordable price point. I even purchased a few extra bottles to gift to friends and family.
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Reviewed in the United States on May 13, 2026
★★★★★ 5
Wow - my new favorites 💜👍😀
Scent: Retinol, Size: 17 Fl Oz (Pack of 1)
I just purchased this and tried it last night for the first time along with the firming version. I didn't take photos because I was skeptical as a product I had purchased from a different line for more money didn't work. This morning I notice that not only does my skin feel more hydrated but the texture appears more smooth. I recently turned 75 & lost my husband a few months ago and having been a care giver for awhile and put taking care of me aside. These products and body wash are great and I like the fragrance.
Please give them a try. To have something show this much difference over night is incredible. They are also affordable which is a plus for me. Thank you Olay.
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Reviewed in the United States on April 24, 2026
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