SKU: 88977783518

Human IFN-γ OneStep HS ELISA Kit

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Description

Human IFN-γ OneStep HS ELISA KitProduct Specification Antigen IFN Immunogen Recombinant Protein Antibody Type Recombinant mAb Reactivity Hu Purification Protein A Stability & Storage 12 months from date of receipt reconstitution, 2 to 8'C as supplied. Kit Precision Intra assay: 4. 5% Inter assay: 6. 0% Sample type Serum, Plasma Assay type Sandwich (quantitative) Sensitivity 0. 316pg ml Range 0. 78 pg mL 50 pg mL Recovery Serum: 94% Plasma: 97% Assay time 60 minutes Species

Product Specification


Antigen IFN-γ
Immunogen Recombinant Protein
Antibody Type Recombinant mAb
Reactivity Hu
Purification Protein A
Stability & Storage

12 months from date of receipt / reconstitution, 2 to 8'C as supplied.

Kit


Precision Intra-assay: 4.5%;
Inter-assay: 6.0%
Sample type Serum, Plasma
Assay type Sandwich (quantitative)
Sensitivity 0.316pg/ml
Range 0.78 pg/mL – 50 pg/mL
Recovery Serum: 94%
Plasma: 97%
Assay time 60 minutes
Species reactivity Human
NIBSC convert

The dose response curve of the International Standard (87/586) parallels the OneStep standard curve. To convert sample values obtained with the kit to approximate NIBSC (87/586) units, use the following equation: NIBSC (87/586) approximate value (IU/mL) = 0.02 x OneStep Human IFNγ value (pg/mL).

Background

Interferon gamma (IFN-γ) is a cytokine critical to both innate and adaptive immunity, and functions as the primary activator of macrophages, in addition to stimulating natural killer cells and neutrophils. A non-IgE-mediated anaphylactic reaction and severe bronchospasm have been reported once after the first injection of interferon gamma. IFN-γ activates cells via a different receptor than IFN-α and IFN-β, which accounts for the different physiological properties of the proteins. Production of IFN-γ is largely restricted to activated CD4+ TH1 T cells, CD8+ T cells, and natural killer cells. One of the most important consequences of IFN-γ secretion is the activation of macrophages. In addition, IFN-γ plays a central role in inflammatory responses by activating endothelial cells, promoting TH1 cell development and cellular immune responses, and up-regulation of major histocompatability complex protein expression on antigen-presenting cells.

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Exchange/Return Notes
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SKU: 88977783518

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