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Description
Human TGFBR2 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Transforming Growth Factor Beta Receptor II (TGFBR2) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Transforming Growth Factor Beta Receptor II (TGFBR2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Transforming Growth Factor Beta Receptor II ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Transforming growth factor beta receptor II (70/80 kDa) is a TGF-beta receptor. It is a tumor suppressor gene. It encodes a member of the serine/threonine protein kinase family and the TGF-beta receptor subfamily. The encoded protein is a transmembrane protein with a protein kinase domain that forms a heterodimeric complex with another receptor protein and binds to TGF-beta. This receptor/ligand complex phosphorylates the protein, which then enters the cell nucleus and regulates the transcription of a subset of genes involved in cell proliferation. Mutations in this gene have been associated with Marfan syndrome, Loeys-Deitz aortic aneurysm syndrome, Osler-Weber-Rendu syndrome, and the development of various types of tumors. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.4 ★★★★★
Based on 16 reviews
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Product Reviews
★★★★★ 3
Slow burn with a sprinkle of fantasy.
Format: Kindle
It was alright. Written well. Story was decent. But it just lacked something.
It's the slowest burn I've ever read, which is fine.
I felt like the knight (main character) was kind of weak and whiney… And I expected the strength of a knight!
The first chapter is filled with a lot of background information, but then it's like the story doesn't really reference it again. I would have liked a bit more information that first chapter before just heading right into the story in chapter 2.
Things could have been explained more in general. There could have been a lot more detail and explanation abiut the fantasy side of the book. It was just like a straight romance with a sprinkle of fantasy.
Overall, it was decent, but I probably wouldn't recommend it to someone unless they asked specifically for a slow burn with a little bit of fantasy.
I received an advance review copy for free, and I am leaving this review voluntarily.
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Reviewed in the United States on November 9, 2024
★★★★★ 4
A Forbidden Romance With Teeth
Format: Paperback
First off: I loved the couple. Della, our acting princess, and her sharp wit and determination to survive were a refreshing change from the damsel type princess. Galeon was a woman of honour and yet so head over heels with the fake princess that she can't keep away.
Birch does a great job of building a magical world and drawing you into it so that you kept turning pages until the end. And that page-turning was also spurred by the slow burn will-they-won't-they romance between the heroines who had much to lose if they crossed the line. I've purchased all three novels and will review Her Blushing Hero just as soon as I've finished.
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Reviewed in the United States on March 11, 2026
★★★★★ 5
AMAZINGGGGG
Format: Kindle
My first sapphic read and i think I’ll forever be attached to her writing! Already finished the consecutive two books in the series and I’m back to rereading them all! Such a great author to draw up such vivid pictures through words, especially the spicy parts! Great slow burn, so much action and lesbians are normalized in this world??? I loveeee it! Thank you Ms. Mariah for giving your readers a beautiful place to escape! Can hardly wait for spring when she releases the 4th book 😍
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Reviewed in the United States on February 21, 2026
★★★★★ 1
Toxic fumes!
Format: Hardcover
Be careful! Book stank, even after a few days with fumes that felt toxic and probably were. Returned
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Reviewed in the United States on December 5, 2025
★★★★★ 5
A HAUNTINGLY BEAUTIFUL TAPESTRY OF EMOTIONS
Format: Paperback
“𝘓𝘰𝘷𝘦 𝘤𝘢𝘯’𝘵 𝘫𝘶𝘴𝘵 𝘥𝘪𝘴𝘢𝘱𝘱𝘦𝘢𝘳 𝘸𝘩𝘦𝘯 𝘵𝘩𝘪𝘴 𝘭𝘪𝘧𝘦 𝘪𝘴 𝘵𝘩𝘳𝘰𝘶𝘨𝘩, 𝘤𝘢𝘯 𝘪𝘵, 𝘑𝘰𝘯𝘢𝘩? 𝘌𝘷𝘦𝘯 𝘪𝘧 𝘰𝘶𝘳 𝘣𝘰𝘥𝘪𝘦𝘴 𝘵𝘶𝘳𝘯 𝘵𝘰 𝘥𝘶𝘴𝘵, 𝘵𝘩𝘦 𝘭𝘰𝘷𝘦 𝘸𝘦 𝘧𝘦𝘦𝘭 𝘮𝘶𝘴𝘵 𝘨𝘰 𝘴𝘰𝘮𝘦𝘸𝘩𝘦𝘳𝘦.”
What a rare gem of a story! With an almost dreamlike, fairy-tale quality to her every word, Mia Sheridan weaves a hauntingly beautiful tapestry of darkness and light, love and loss, pain and atonement, with a tale that transports the reader to a place where true love never dies, and where a spark of hope can find its way through even the smallest of cracks in an old, stone wall. Set against the vivid background of the mystical city of New Orleans, this is the story of two lonely hearts who find safe harbour in each other’s arms, but as they work together to unlock a mystery from the past, they begin to discover that the greatest battles sometimes lie within us, and that is where they must be fought. As always, Mia Sheridan’s storytelling is exquisite, transcendent, soulful, and just stunning.
𝘊𝘭𝘢𝘳𝘢 𝘩𝘢𝘥 𝘣𝘦𝘤𝘰𝘮𝘦 𝘤𝘢𝘱𝘵𝘪𝘷𝘢𝘵𝘦𝘥—𝘱𝘦𝘳𝘩𝘢𝘱𝘴 𝘦𝘷𝘦𝘯 𝘢 𝘭𝘪𝘵𝘵𝘭𝘦 𝘰𝘣𝘴𝘦𝘴𝘴𝘦𝘥—𝘸𝘪𝘵𝘩 𝘵𝘩𝘦 𝘵𝘢𝘭𝘦 𝘰𝘧 𝘩𝘦𝘢𝘳𝘵𝘣𝘳𝘦𝘢𝘬 𝘢𝘯𝘥 𝘮𝘪𝘴𝘦𝘳𝘺 𝘵𝘩𝘢𝘵 𝘩𝘢𝘥 𝘰𝘤𝘤𝘶𝘳𝘳𝘦𝘥 𝘮𝘰𝘳𝘦 𝘵𝘩𝘢𝘯 𝘢 𝘩𝘶𝘯𝘥𝘳𝘦𝘥 𝘢𝘯𝘥 𝘧𝘪𝘧𝘵𝘺 𝘺𝘦𝘢𝘳𝘴 𝘣𝘦𝘧𝘰𝘳𝘦.
Shortly after moving to New Orleans to pursue a career in ballet, Clara Campbell finds herself awash in loneliness and uncertainty, the company of an elderly neighbour becoming her only respite from the melancholy that threatens to overwhelm her daily. And it is during one of her visits to her elderly friend that Clara learns of a century-and-a-half-old legend held within the beautiful, broken structures of Windisle Plantation. From the moment Clara hears the story of a beautiful young slave who fell for a handsome southern soldier, and whose spirits are said to be still trapped in the very place where their love affair ended so tragically, she begins to feel a strange pull to Windisle, beckoning her to explore the secrets held within its shadows. But instead of finding ghosts there, Clara meets a mysterious stranger hiding behind its walls.
𝘍𝘰𝘳 𝘯𝘰𝘸 𝘵𝘩𝘦𝘺 𝘸𝘦𝘳𝘦 𝘫𝘶𝘴𝘵 𝘢 𝘣𝘰𝘺 𝘢𝘯𝘥 𝘢 𝘨𝘪𝘳𝘭, 𝘴𝘪𝘵𝘵𝘪𝘯𝘨 𝘰𝘯 𝘰𝘱𝘱𝘰𝘴𝘪𝘵𝘦 𝘴𝘪𝘥𝘦𝘴 𝘰𝘧 𝘢 𝘸𝘢𝘭𝘭, 𝘢 𝘭𝘢𝘺𝘦𝘳 𝘰𝘧 𝘵𝘩𝘪𝘤𝘬 𝘴𝘵𝘰𝘯𝘦 𝘣𝘦𝘵𝘸𝘦𝘦𝘯 𝘵𝘩𝘦𝘮, 𝘣𝘶𝘵 𝘵𝘩𝘦𝘪𝘳 𝘩𝘦𝘢𝘳𝘵𝘴 𝘤𝘰𝘯𝘯𝘦𝘤𝘵𝘦𝘥 𝘯𝘰𝘯𝘦𝘵𝘩𝘦𝘭𝘦𝘴𝘴. 𝘈𝘯𝘥 𝘧𝘰𝘳 𝘯𝘰𝘸, 𝘩𝘦 𝘸𝘰𝘶𝘭𝘥 𝘦𝘯𝘫𝘰𝘺 𝘵𝘩𝘦 𝘮𝘰𝘮𝘦𝘯𝘵𝘴 𝘵𝘩𝘦𝘺 𝘩𝘢𝘥.
Jonah Chamberlain hasn’t stepped outside his old, crumbling family estate in eight long, grief-filled years. Holding himself responsible for the tragic events that led to his own scarred appearance, Jonah spends his days drowning in sorrow and loneliness, but from the moment he hears a young woman’s selfless wish whispered through the cracks in an old, stone wall, he becomes bewitched by her. They continue to meet week after week, spending time just talking, and sharing their innermost thoughts and fears with one another, even though that stone wall continues to stand between them.
“𝘐 𝘳𝘦𝘨𝘳𝘦𝘵. 𝘐 𝘫𝘶𝘴𝘵 𝘳𝘦𝘨𝘳𝘦𝘵. 𝘐’𝘷𝘦 𝘮𝘢𝘥𝘦 𝘢 𝘤𝘢𝘳𝘦𝘦𝘳 𝘰𝘧 𝘪𝘵, 𝘩𝘦𝘳𝘦, 𝘣𝘦𝘩𝘪𝘯𝘥 𝘵𝘩𝘪𝘴 𝘸𝘢𝘭𝘭.”
As they slowly begin to piece the past together, getting closer and closer to setting the star-crossed lovers free from the curse trapping them at Windisle, Clara and Jonah also begin to fall in love. But it soon becomes clear to Clara that her velvet-voiced ‘wish collector’ might be living in a cage of his own making—never too far, but always far enough to be out of reach to her, their reality in so many ways echoing the very curse they are trying to break.
𝘞𝘩𝘢𝘵 𝘢𝘯 𝘢𝘸𝘧𝘶𝘭, 𝘥𝘦𝘴𝘰𝘭𝘢𝘵𝘦 𝘧𝘦𝘦𝘭𝘪𝘯𝘨, 𝘵𝘰 𝘬𝘯𝘰𝘸 𝘵𝘩𝘢𝘵 𝘵𝘩𝘦 𝘮𝘢𝘯 𝘸𝘩𝘰 𝘮𝘢𝘥𝘦 𝘺𝘰𝘶𝘳 𝘩𝘦𝘢𝘳𝘵 𝘴𝘸𝘦𝘭𝘭 𝘢𝘯𝘥 𝘺𝘰𝘶𝘳 𝘣𝘭𝘰𝘰𝘥 𝘵𝘳𝘦𝘮𝘣𝘭𝘦 𝘸𝘢𝘴 𝘵𝘩𝘦𝘳𝘦, 𝘴𝘰 𝘤𝘭𝘰𝘴𝘦, 𝘢𝘯𝘥 𝘺𝘦𝘵 𝘤𝘰𝘮𝘱𝘭𝘦𝘵𝘦𝘭𝘺 𝘢𝘯𝘥 𝘶𝘵𝘵𝘦𝘳𝘭𝘺 𝘰𝘶𝘵 𝘰𝘧 𝘳𝘦𝘢𝘤𝘩. 𝘞𝘩𝘢𝘵 𝘵𝘰𝘳𝘵𝘶𝘳𝘦 𝘵𝘰 𝘸𝘰𝘯𝘥𝘦𝘳 𝘪𝘧 𝘩𝘦 𝘮𝘪𝘨𝘩𝘵 𝘣𝘦 𝘭𝘰𝘰𝘬𝘪𝘯𝘨 𝘧𝘰𝘳 𝘺𝘰𝘶 𝘵𝘰𝘰.
The depth of emotion that Mia Sheridan is able to conjure on the page is breathtaking, her nuanced prose brimming with symbolism and sensuality, but her true gift lies in creating complex, fractured characters that have been bruised by life, but who find unexpected glimmers of hope and grace when they least expect it. The novel builds slowly with absolutely gorgeous language and setting, and with a dual timeline structure that sways gently from past to present, braiding the narratives together little by little, we unravel the past at the same time as the future unfolds before us. Both beautiful and tragic, this is the kind of story that sticks with you long after the final page.
“𝘛𝘩𝘦𝘳𝘦’𝘴 𝘢 𝘱𝘭𝘢𝘤𝘦 𝘧𝘰𝘳 𝘶𝘴, 𝘈𝘯𝘨𝘦𝘭𝘪𝘯𝘢. 𝘚𝘰𝘮𝘦𝘸𝘩𝘦𝘳𝘦 𝘪𝘯 𝘵𝘩𝘪𝘴 𝘸𝘪𝘥𝘦, 𝘸𝘪𝘥𝘦 𝘸𝘰𝘳𝘭𝘥. 𝘋𝘰 𝘺𝘰𝘶 𝘵𝘳𝘶𝘴𝘵 𝘮𝘦?”
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Reviewed in the United States on December 12, 2018