SKU: 91465641755

UA-Myco Nest PCR Mycoplasma Detection Kit

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Description

UA-Myco Nest PCR Mycoplasma Detection KitProduct Specification Synonyms Nested PCR Mycoplasma Detection Kit Stability & Storage Transported with ice packs. Store at 20 away from light, with a validity period of 18 months. Background The UA Myco Nested PCR Mycoplasma Detection Kit is used for the qualitative detection of mycoplasma contamination in cell cultures. Over 95% of mycoplasma contamination in cell cultures is caused by Mycoplasma fermentans, M. orale, M. pirum, M. hyorhinis, M.

Product Specification


Synonyms Nested PCR Mycoplasma Detection Kit
Stability & Storage

Transported with ice packs. Store at -20℃ away from light, with a validity period of 18 months.

Background

The UA-Myco Nested PCR Mycoplasma Detection Kit is used for the qualitative detection of mycoplasma contamination in cell cultures. Over 95% of mycoplasma contamination in cell cultures is caused by Mycoplasma fermentans, M. orale, M. pirum, M. hyorhinis, M. hominis, M. salivarium, M. arginini, and Acholeplasma laidlawii. This kit amplifies the conserved gene fragments of these eight and many other common mycoplasmas in cell culture media using the nested PCR method to determine the presence of mycoplasma contamination. The kit features rapidity, high sensitivity, and high specificity. Each reaction can detect as few as 10 mycoplasma gene copies, with particularly significantly improved detection sensitivity for Acholeplasma laidlawii, and there is no cross-reactivity with bacteria or cultured cells.

Components

The nested PCR mycoplasma detection kit contains 2 tubes of ready-to-use primers and 1 tube of reaction internal control, with specifications as follows:

Specification

component

25T

One tube of Primer P1 (50 μL), one tube of Primer P2 (50 μL), one tube of PCR reaction internal reference (50 μL)

50T

1 tube of Primer P1 (100 μL), 1 tube of Primer P2 (100 μL), 1 tube of PCR reaction internal reference (100 μL)


Protocol

1. Preparation of cell culture medium to be tested
1) Take 1mL of cell culture medium from the cell culture in the logarithmic growth phase.
2) Centrifuge the cell culture medium at 200g for 5 minutes at room temperature. Transfer approximately 800μL of the medium to a new centrifuge tube, taking care not to introduce the precipitated cell debris.
3) Use the prepared cell culture medium as a template for nested PCR to detect mycoplasma.
2. Nested PCR reaction
1) First PCR reaction: Take the general 2X PCR premix reagent as an example:
PCR reaction assembly (preparation on ice is recommended))

component

Volume (μL)

2xPCR Mix                                     

25

1st PCR primer P1

2

Template (cell culture medium)

1

PCR reaction internal reference

2

Add sterile ultrapure water to                              

50

 

PCR reaction conditions: 30 cycles, conditions are shown in the table below

Step   

temperature

time

cyclic number

Pre-denaturation                            

94℃

5min

1

transgender               

94℃

30s

30

annealing           

55℃

30s

30

extend             

72℃

40s

30

Finally extends      

72℃

 5min

1

 

2) Take 1 μL from the first PCR reaction as the template for the second PCR and perform the second PCR using primer P2. The assembly of the second PCR reaction (preferably prepared on ice) is shown in the table below. The PCR reaction conditions are the same as those for the first PCR reaction.

component                                       

Volume (μL)

2xPCR  Mix                                   

25

2nd PCR primer P2

2

Template (1st  PCR product)

1

Add sterile ultrapure water to                                   

50

 

3) Prepare a 2% agarose gel. Take 10µl of the second PCR product for DNA electrophoresis.

3. Results
The internal control of the PCR reaction shows a single DNA band at 515bp. Common mycoplasma contamination produces a single DNA band ranging from 236bp to 365bp. Acholeplasma laidlawii contamination results in double DNA bands at 426bp and 219bp.

Guidelines

It is recommended that the PCR reagents be from the hot-start Taq enzyme series. The 2X PCR premix used should not contain DNA gel electrophoresis stain.

It is recommended to set up the PCR reaction in a clean bench to avoid cross-contamination and aerosol contamination. Use nuclease-free pipette tips and reaction tubes; it is advisable to use filter tips.

Without strict verification, it is not recommended to arbitrarily change the amounts of reaction reagents.

Aliquot and store the reaction reagents according to the instructions to ensure the stability of the reagents.

This product is for research use only.

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SKU: 91465641755

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B
Verified Purchase
B. Stubby
Draper, US
★★★★★ 3
A familiar story, just with…..less.
Format: Kindle
So, as other reviewers make clear, this is very similar to Pack Darling and The Beta. It’s much closer aligned with The Beta, in plot and maybe more like Pack Darling with characters. That being said, I don’t hate this…..but it wasn’t great either. It’s both books mentioned but just….less. Less angst, less emotion, less feeling. The plot feels very half fleshed out, and the “bad guy” feels underwhelming. I didn’t really feel any real emotions from and of the male leads, except maybe Oliver. The others fell sorta flat for me. And Mika makes herself out to be this big bad ass straight outta training and then we never see it from here again with the one fitting room incident as the exception. SPOILER: The whole, “Oh, I’m actually probably an Omega, but I don’t wanna be but I do actually wanna be but no one can ever know my secret that I do nothing to hide “ thing fell so flat. She never commutes to believing she was secretly an omega, but also mentions her “secret” a lot. It just felt so manufactured. I’m intrigued enough to read part 2 and see how the author closes everything out, but this is not one I’ll recommend or ever come back to.
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Reviewed in the United States on February 13, 2024
R
Verified Purchase
Ruth Ann Burt
San Leandro, US
★★★★★ 5
Great book
Format: Kindle
I absolutely feel in love with all 4 characters!!! The bedroom scenes were 🌋🌡🔥🔥🔥. I couldn't put this book down!!! I'm hooked for the whole series Book 2 here I come!!!!! Its a fun easy book and story to read!!
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Reviewed in the United States on October 4, 2024
D
Verified Purchase
Danyelle
Houston, US
★★★★★ 4
Fun with a late blooming omega
Format: Kindle
I like this book. The story is fun, cute, and sexy. There's just a little drama, some excellent, steamy scenes, and a fairly good relationship building storyline. I especially like how all the main characters are a bit older than the usual 20 somethings I tend to see in this kind of book. Having said that, I wish there were more descriptions of the places, as well as the food in the fancy restaurant. I enjoyed the cocktails at the club, so I missed that kind of detail when Gray took Madison on a dinner date. I also wish there had been more interaction between Lucas and Madison, and Lucas and Rian. It felt a bit lopsided, with a focus on Rian, Madison, and Gray. I wish it had been proofread - there are a lot of typos, but nothing too distracting.
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Reviewed in the United States on September 12, 2022
J
Verified Purchase
Jennifer G
Charlottesville, US
★★★★★ 3
Madison Deserved Better
Format: Kindle
Madison was a beta...except she wasn't any longer. She was a late presenting Omega. And she was struggling. She was tall and thin, not tiny and curvy. She was opinionated. She was everything an Omega was not. After suffering through her first heat, her friends took her to Ardor, a club where Omegas came to safely find Alphas. She's not expecting much but then she connects with a sexy beta. And when she meets his Alphas, they set her body on fire. Maybe, she's found her no-strings-attached heat pack. Maybe, she's found something more. I could not connect with the characters in this book, so their story never resonated with me. And there was no love story; there was sex. Grey made it clear from the beginning that he had a true love and it was his beta boy, Rian. He went so far as to reassure Rian “Say the word, I’ll never touch her again. Lucas can put the babies in her. I only need you, beta boy”. So, Madison was there for babies, no emotions needed. Nice. No, thank you. I want the Omega to be the center of their world, not an incubator. Lucas and Rian weren't any better. After her heat, they let her leave. Not one of them made her feel valued. No one gave her a reason to stay or even offered a cuddle. And the sex didn't even come across as mind-blowing. Madison deserved better.
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Reviewed in the United States on March 11, 2025
O
Verified Purchase
Oregon BookWorm
Carnegie, US
★★★★★ 5
No breakup, very sweet, instalove
Format: Kindle
Omegaverse and doesn't disappoint! Sweet guys, newly Omega FMC. The boyfriends are boyfriends. What's not to love? No angst, no breakup.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on February 23, 2025

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