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Description
β-actin Recombinant Rabbit mAb (Cy5 Conjugate) (SDT-R156)Product Specification Host Rabbit Antigen Actin Synonyms ACTB Immunogen N A Location Cytoplasm, Cytoskeleton Accession P60709 Clone Number SDT R156 Antibody Type Recombinant mAb Application ICC, ICFCM Reactivity Hu, Ms, Rt Purification Protein A Concentration 2 mg ml Conjugation Cy5 Physical Appearance Liquid Storage Buffer PBS, 0. 1% BSA, 0. 01% Proclin 300 Stability & Storage 12 months from date of receipt reconstitution, 2 to 8 C as supplied.
Product Specification
| Host | Rabbit |
| Antigen | β-Actin |
| Synonyms | ACTB |
| Immunogen | N/A |
| Location | Cytoplasm, Cytoskeleton |
| Accession | P60709 |
| Clone Number | SDT-R156 |
| Antibody Type | Recombinant mAb |
| Application | ICC, ICFCM |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Cy5 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 0.1% BSA, 0.01% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| ICC | 1:200 | |
| ICFCM | 1:2000 |
Background
Beta-actin (human gene and protein abbreviation ACTB/ACTB) is one of six different actin isoforms which have been identified in humans. This is one of the two non-muscle cytoskeletal actins. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actin is often used in Western blotting as a loading control, to normalize total protein amounts and check for eventual protein degradation in the samples. Its transcript is also commonly used as a housekeeping gene standard in qPCR. Its molecular weight is approximately 42 kDa.
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