SKU: 93679546862

Human IFN-γ High Sensitivity ELISA Kit

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Description

Human IFN-γ High Sensitivity ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti human ifn Capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The ifn &gamma in the sample;

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti human ifn-γ Capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The ifn-&gamma in the sample; Bound to solid-phase antibody and detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response and ifn-&gamma in the sample; There is a positive correlation between the concentration of. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: Pre coated 96 well plates, standards, ifn-γ One copy of detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.04 pg/ml

Detection Range: 0.39-25 pg/ml

Recovery Range: 85-94%

Storage Method: 2-8 ℃

Standard Curve:

Background:

γ Interferon (IFN-γ)Is a soluble dimeric cytokine and is the only member of type II interferons. It is mainly secreted by natural killer (NK) and natural killer T (NKT) cells and plays a role in innate immunity; It is secreted by cd4th1 and CD8 cytotoxic T cells during antigen-specific immunity. IFN-γ Or type II interferons play an important role in innate and adaptive immunity against virus, some bacteria and protozoan infections. IFN-γ It is an important activator of macrophages and an inducer of type II major histocompatibility complex (MHCII) expression. IFN-γ The abnormal expression of Bcl-2 is associated with many autoinflammatory and autoimmune diseases. In addition to directly inhibiting viral replication, ifn-γ Its importance to the immune system is more reflected in its immune stimulation and immune regulation functions.

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SKU: 93679546862

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Sydney
West Palm Beach, US
★★★★★ 4
Cute but not durable
Color: Blue Octopus, Color: Blue Octopus
My dog LOVES the toy and was so excited to get it. It is so cute…but not durable like advertised. My chewer was able to rip a hole by the evening we received it. We like it…just not excited for the clean up
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Reviewed in the United States on March 16, 2026
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MARIA
Houston, US
★★★★★ 5
Perfect Teething Toy for Puppies!
Color: Pacifier, Size: Small
My puppy absolutely loves this Nylabone Puppy Pacifier Chew Toy! The bacon flavor immediately caught his attention, and it has quickly become his favorite toy. It’s the perfect size for small puppies and easy for little mouths to carry around and chew on. The material is soft enough for teething but still durable enough to hold up to daily chewing. It has really helped redirect my puppy from chewing on furniture and shoes, and it seems to soothe his gums while he’s teething. I also like that it keeps him entertained for long periods of time. Great quality, puppy-approved, and definitely worth buying for any small dog owner!
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Reviewed in the United States on May 29, 2026
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Bryanne Frye
San Leandro, US
★★★★★ 5
My dogs love these
Color: Pacifier, Size: Small, Color: Pacifier, Size: Small
These are my dogs favorite toys and they keep them entertained for hours. Yes they are able to chew them down to small pieces but they are sturdy enough where they won't swallow them. I have miniature dachshunds so small dogs.
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Reviewed in the United States on May 2, 2026
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Donna Mick
Draper, US
★★★★★ 5
Great chew toy for my dog!
Color: Pacifier, Size: Small
My dog loves his Pacifier! It is pretty durable, odorless, easy for him to use and carry around from room to room! He loves to play with it!
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Reviewed in the United States on June 3, 2026
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tara speece
Waukegan, US
★★★★★ 4
Perfect for Teething Puppies and Adult Dogs and Keeps Them Busy
Color: Pacifier, Size: Small, Color: Pacifier, Size: Small
The Nylabone Puppy Pacifier Chew Toy has been a lifesaver during my puppy’s teething stage and AFTER. Quality: The material is sturdy yet gentle enough for developing puppy teeth. After weeks of daily use, it shows normal wear. The pacifier shape makes it easy for small mouths to grip and carry around. Effectiveness: It’s great for soothing sore gums and redirecting chewing behavior. I’ve noticed my puppy is calmer after chewing on it for a while. Helpfulness Tip: Supervise during chewing sessions and wash it occasionally with warm soapy water to keep it clean. If you have a small-breed puppy under 25 lbs, this toy is a safe, durable, and fun way to help with teething. Bought twice, they love these. Miniature dachshund and French bulldog
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Reviewed in the United States on August 13, 2025

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