SKU: 94008934307

Rat IL-10 High Sensitivity ELISA Kit

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Description

Rat IL-10 High Sensitivity ELISA KitProduct Specification Description Detection Principle: This kit uses double antibody sandwich ELISA technology. Specific anti rat IL 10 capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL 10 in the sample binds to the solid phase antibody. After

Product Specification

Description

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti rat IL-10 capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-10 in the sample binds to the solid-phase antibody. After thorough washing to remove free and unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-10 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: a copy of pre coated 96 well plate, standard, biotin labeled IL-10 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.22 pg/ml

Detection Range: 6.25-400 pg/ml

Recovery Range: 94-115%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 10 (IL-10) is a homodimer composed of two 178 amino acid subunits. In humans, it is encoded by the IL-10 gene located on chromosome 1 and consists of five exons. IL10 is mainly secreted by monocytes, lymphocytes, type II helper T cells (Th2), mast cells, cd4+cd25+foxp3+ regulatory T cells and partially activated T cells and B cells secreted less. It has multiple effects on immune regulation and inflammation. It downregulates Th1 cytokines, MHC class II antigens and costimulatory molecules of macrophages. It can also enhance B cell survival, proliferation and antibody secretion. IL-10 can block NF - & kappa; B activity, and participate in JAK-STAT signaling pathway regulation. The immunosuppressive properties of IL-10 suggest that it may be used in clinic to inhibit rejection after organ transplantation. IL-10 also has strong anti-inflammatory properties.

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SKU: 94008934307

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4.5 ★★★★★
Based on 22 reviews
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Verified Purchase
Diane Lee
Fort Morgan, US
★★★★★ 4
Great little temper
Size: 53mm, Color: Silver
Works great! Only problem is that it doesn’t have that feel of tamping. Wish I had more feedback. It just goes down very easily and doesn’t have that feeling of solid tamping. Now that doesn’t mean it’s not doing its job. Works fine but would like some manual feedback.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on November 7, 2024
J
Verified Purchase
Johamis
Lake Worth, US
★★★★★ 5
Easy to use
Size: 58.3mm, Color: Black
Great for make good coffee and easy to use.
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Reviewed in the United States on October 24, 2025
J
Verified Purchase
JK
Battle Creek, US
★★★★★ 5
Take the guess work out of the flow!
Size: 58.3mm, Color: Black
I got this because when my wife makes espresso, it's watery. When i make it, it's fine. We needed a controlled Tampering method so we both do the same job. This is heavy, and being able to use the whole hand instead of fingers, not much effort is needed. Since we purchased this, we were able to make consistent espresso shots for sure.
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Reviewed in the United States on July 12, 2024
R
Verified Purchase
Reviewer JR
Cuba, US
★★★★★ 3
Not as good as I expected
When it first came, the box fell apart as I was taking it out, and a lot of some kind of dirt or sand kept falling out all over my table and floor. I had the take the entire thing apart and thoroughly wash it. After drying age reassembling it, I tried it on my portafilter that came with my Breville Barista Express, but, somehow, it doesn't always make the tamping level. You would think with the ridge that rests on the side of the portafilter that it would force it to remain level, but, somehow, it doesn't, at least not consistently. It also has varying lengths (heights, or depths), and a single spring tension, so you may still over tamping or under-tamp. I was expecting it would have a censor that would just detect once it reached 30 lbs of force and cut off after that, similar to a torque screwdriver, but that's not what this is. I'll still give it 3 stars because, despite the problems mentioned above, it at least produces some amount of consistency, even if that isn't measurable; but, I can't give it 5 stars because of those issues mentioned above.
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Reviewed in the United States on January 7, 2025
B
Verified Purchase
Bernard Eisenfeld, M.D.
Birmingham, US
★★★★★ 5
This is the one.
Size: 58.3mm, Color: Black
As an el Supremo Coffee Geek, I've wasted money on lots of different tampers. Save your money and buy this one or it's equivalent. This thing is heavy duty, solid, well-made, self-leveling, tamps at 30 pounds and is fool-proof even for fools. Also, it's a palm tamper meaning more fun and more satisfying to use as you prep the puck for your morning buzz. Buy it. You will thank me.
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Reviewed in the United States on March 30, 2024

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