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Description
UniOne® TR-FRET Human VAV1/CRBN PROTAC Binding KitProduct Specification Host Human Stability & Storage 80C Background The kit employs homogeneous time resolved fluorescence (TR FRET) technology to measure the interaction between the DDB1 CRBN complex and VAV1 mediated by the molecular glue MRT 6160 or test compounds. This method provides a high throughput format for the simple and rapid detection of small molecules capable of mediating the interaction between the DDB1 CRBN complex and VAV1. As shown,
Product Specification
| Host | Human |
| Stability & Storage | -80°C |
Background
The kit employs homogeneous time-resolved fluorescence (TR-FRET) technology to measure the interaction between the DDB1/CRBN complex and VAV1 mediated by the molecular glue MRT-6160 or test compounds. This method provides a high-throughput format for the simple and rapid detection of small molecules capable of mediating the interaction between the DDB1/CRBN complex and VAV1.
As shown, the interaction between DDB1/CRBN and VAV1 is detected using a Eu-labeled anti-Tag1 antibody (TR-FRET donor) and an Accepter-labeled anti-Tag2 antibody (TR-FRET acceptor). Due to the molecular glue MRT-6160 mediating the interaction between DDB1/CRBN and VAV1, the donor and acceptor antibodies are brought into close proximity. Excitation of the donor antibody triggers fluorescence resonance energy transfer (FRET) to the acceptor antibody, causing the acceptor antibody to specifically emit a signal at a wavelength of 665 nm. This specific signal is proportional to the extent of the interaction mediated by MRT-6160 between DDB1/CRBN and VAV1. This homogeneous assay is simple to perform and requires no washing steps.
Components
Component |
Concentration |
100T |
500T |
2500T |
10000T |
Storage Temperature |
Tag1-DDB1/CRBN protein |
100× |
5μL |
20μL |
100μL |
400μL |
-80℃ |
Tag2- VAV1 protein |
100× |
5μL |
20μL |
100μL |
400μL |
-80℃ |
MRT-6160 （200μM） |
100× |
5μL |
10μL |
50μL |
200μL |
-80℃ |
Anti-Tag1 Eu antibody |
50× |
10μL |
50μL |
250μL |
1000μL |
-80℃ |
Anti-Tag2 Ac antibody |
100× |
5μL |
25μL |
125μL |
500μL |
-80℃ |
Detection buffer |
10× |
400μL |
2mL |
10mL |
40mL |
-80℃ |
Note: Aliquot immediately after the first thaw and store at the recommended storage temperature. Avoid storage after dilution and repeated freeze-thaw cycles.
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
- Except Preorder products are shipped in 48 hours.
- Delivery to the USA:
- Standard Shipping : 3-10 business days
- If time is of the essence, please consider selecting expedited delivery for faster service.
Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy