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Description
Human TGM3 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Gently wash adherent cells with pre-chilled PBS, then trypsinize and collect the cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash the collected cells three times with pre-chilled PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Collect the supernatant for analysis, or store at -20°C or -80°C, but avoid repeated freezing and thawing. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a transglutaminase 3, epidermal (TGM3) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of transglutaminase 3, epidermal (TGM3) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Transglutaminase 3, Epidermal ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Protein-glutamine gamma-glutamyltransferase E, also known as epidermal transglutaminase, is an enzyme encoded by the TGM3 gene. Transglutaminases catalyze the cross-linking of proteins via epsilon-gamma glutamyl-lysine isopeptide bonds. Although the primary structure of transglutaminases is not conserved, they all share a common amino acid sequence in their active sites, and their activity is calcium-dependent. The protein encoded by this gene consists of two polypeptide chains and is activated from a precursor protein by proteolysis. The encoded protein is involved in the later stages of cell envelope formation in the epidermis and hair follicles. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, cell culture supernatants, and other biological fluids |
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4.5 ★★★★★
Based on 9 reviews
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Product Reviews
★★★★★ 5
Hold a lot
Great rack, holds lots of spice bottles. Sturdy and easy to assemble
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 3, 2026
★★★★★ 4
Easy to assemble but overpriced for what you get
I like this rack. Especially because it was very easy to assemble. Although it is only suitable for one size spice bottles. It wont hold larger or taller spices which was a small issue for me. But overall i found it good quality. But i think the price is a bit overpriced for what you receive and for the functionality
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Reviewed in the United States on May 25, 2026
★★★★★ 5
Excellent way to store spices
In the description it clearly states only 4 oz spice jars are recommended, so keep that in mind when ordering. I also included in my pictures one of their pictures showing the size recommendation for your cabinet, that way you can check that too before ordering. Doing both of these things will provide a happier buying experience and minimize frustration with the product itself.
I already had a tiered spice rack that fit my larger and bulk spices beautifully. I felt having my larger spices with my smaller spices made it difficult to access certain items and it just felt chaotic. To solve that issue I order this spice rack organizer for the smaller spices. It was super easy to assemble. All hardware is included and in addition to the hardware to put this together they also included screws, that way if you would like to screw the spice rack into the cupboard itself you can. I did not choose to screw the spice rack down. I have had no issues with it moving around when I use the pull out.
It pulls in and out smoothly and with barely any effort. The pull out design I feel maximizes space and allows you to see everything all the way to back of the rack. This rack is great for any size space and holds a lot of spices. I had additional room to more spices that I did not need even after adding all the small spices that would fit into each of the pull outs.
I feel the price reflects the quality of the product. Yes it is a steep price and can be viewed as expensive, on the other side you get what you paid for. It is aesthetically pleasing and I am planning on at some point buying spice jars that you fill yourself to use on this spice rack. Eventually I am hoping to be more organized and even have my bulk spices be placed in the individual jars that I fill myself while keeping the bulk container in my pantry. I am extremely pleased with this heavy duty spice rack and do recommend it to others.
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Reviewed in the United States on April 8, 2026
★★★★★ 5
Sturdy, Space-Saving Organizer That Makes Cabinets Way More Functional
The **BOIVSHI 2-Tier Vertical Pull-Out Spice Rack Organizer** is a really practical upgrade if you’re trying to clean up a cluttered cabinet or pantry. The vertical design makes excellent use of narrow spaces, and the **smooth sliding rails** are one of the best parts—it pulls out cleanly so you can actually see everything instead of digging through shelves.
The metal frame feels solid and heavy-duty, and the chrome finish gives it a clean, modern look that blends well inside most cabinets. It’s especially helpful for organizing spice jars, oils, sauces, or small pantry items so everything stays upright and easy to grab.
Installation is fairly simple, and once mounted, it feels stable when sliding in and out. The motion is smooth enough that it doesn’t feel like it’s going to jam or tip items over, which is a common issue with cheaper pull-out racks.
One thing to keep in mind is sizing—this type of organizer works best in **standard-width cabinets**, so measuring your space first is important. Also, like most pull-out systems, it’s more about **function and accessibility** than maximizing sheer storage volume.
Overall, it’s a very effective way to turn wasted cabinet space into organized, accessible storage. If you’re tired of stacked spice jars or cluttered shelves, this is a strong upgrade.
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Reviewed in the United States on April 20, 2026
★★★★★ 3
A Sturdy Spice Rack With Some Perks and Some Limitations
We really needed a new spice rack that holds a variety of bottle sizes, and this one is made for a pretty brand-specific bottle style. It does hold some of the older model metal containers, so it looks like we will be adapting to the newer sizes and shapes. This organizer is easy to assemble and fits the space I designed perfectly. The bottom shelf is very sturdy, and the top shelf is not quite as solid, and I'm not sure exactly why.
One thing is for sure: when you fill this spice rack to capacity, you will have all the spices necessary for a well-stocked kitchen. We have not filled it up, but my guess is that we can fit between 35 and 40 individual spice bottles and cans.
Overall, it is at the higher end of value for the money, but it may serve us perfectly through the years and prove itself to be an excellent value and keep all of our spices well organized.
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Reviewed in the United States on May 16, 2026
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