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Description
Mouse IL19 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 500pg/mL). Then dilute to the following concentrations: 500pg/mL, 250pg/mL, 125pg/mL, 62.5pg/mL, 31.25pg/mL, 15.625pg/mL, 7.8125pg/mL, and 0pg/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each tube. Pipette 500uL of the 500pg/mL standard working solution into the first EP tube and mix thoroughly to make a 250pg/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with Interleukin 19 (IL19) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of Interleukin 19 (IL19) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Interleukin 19 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Interleukin-19 (IL-19) is a protein that belongs to the IL-10 cytokine subfamily. IL-19 is encoded by the IL-19 gene located on chromosome 1. The IL-19 gene consists of seven exons. The secreted IL-19 protein is composed of an α-helical structure consisting of 159 amino acids. IL-19 is an immunosuppressive cytokine responsible for downregulating cell-type-specific and system-specific inflammation. It interacts with both immune cells (macrophages, T cells, B cells) and non-immune cells (endothelial cells and brain-resident glial cells, among others). | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 7.8-500 pg/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.6 ★★★★★
Based on 147 reviews
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Product Reviews
★★★★★ 4
$500, not $600. It isn’t worth $600
Color: White
You’re gonna need to put on some glasses and grab a drink for this one.
Cons :
- Has issues with “muddy mats” brand rugs and mats, along with light mats
- It ate my socks
- Will molest your standing fans and shoes and pet toys
- Castor wheel is a hair magnet along with the “tangle less” floating brush
- Mop heads get dirty after just one run of the entire house
- It hates charging cables
- And dryer sheets
PROS:
- Smarter than Shark brand vacuums
- Excellent management of houses and avoiding most obstacles
- It mops and vacuums well
- Cleaning is very easy
- Cat litter gets sucked up on the strong setting (not the super strong, though that works too)
- Battery lasts for vacuuming most of the house
- No navigation issues
- Doesn’t mess up your furniture
- IT WORKS IN THE DARK
the floating brush though where the metal shaft sits inside the brush itself and the miniscule space between the spinning part and the brush itself gathers hair like a magnet and will give constant roller brush errors if not regularly cleaned. The castor wheel I swear has more hair than anything I’ve seen. Even the shark didn’t collect it this bad.
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Reviewed in the United States on April 17, 2026
★★★★★ 5
Value for Money- Exceptional performance and features!!!!
This vacuum/mop system is wonderful! I highly recommend it. This is bi-far the best vacuum and moping system which effectively cleans my floors and carpet. I've always been on the lookout for anything that can lighten my cleaning load.
it’s been a real game-changer for my household routine. The self-washing and self-charging features are a huge win. I no longer have to worry about cleaning the mop or making sure it’s charged; it takes care of itself.
It moves around furniture and other obstacles smoothly, rarely bumping into anything. It easily navigates tight spaces and corners, making sure every area gets cleaned.
Value for Money: Considering all its features and how well it cleans, I think it’s a fantastic investment. It’s made keeping my home clean so much easier and less time-consuming.
The robot vacuum was able to map my house successfully, the battery life is a bit longer than my previous robot vacuums, the suction is also great. I saw it go over crumbs in the kitchen and it picked up without any problems, and also has a mop functionality. It has a functioning app, very easy to use. The vacuum is also very quiet. By far, this is my favorite robot vacuum and I’ve tried many as I do not like to go around the house mopping and vacuuming. 10/10 would recommend this model - worth the investment.
Overall I could not be happier with this purchase, my home is the cleanest it has been since I’ve first moved in.
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Reviewed in the United States on May 12, 2025
★★★★★ 5
A blessing for “unforgiving” flooring
We bought an 1800s home that was 90% hardwood, but the previous owner made the decision to tear out the hardwood in the kitchen. Unfortunately for us, the flooring we picked to replace it was what I call a very unforgiving. I didn’t realize it until it was too late, but it shows every speck of dust and every muddy paw print. With two dogs, my floors looked like they had not been cleaned in years, but it was being mopped on a daily basis.
Enter the Narwal. I’ve included a photo of what the floor looks like today post-clean on a typical winter day in the north (dogs and people running in and out, tracking and mud and snow everywhere). Look at it! It has a shine! In an hour, it will be dull again from the dogs’ next foray into the snowy yard, but right now…glorious! But the best thing is, I no longer care about the dogs tracking up the floor. I can just run the Narwal again. No real extra labor on my part (other than occasionally needing to fill the water container).
I want to add a couple of points that I find interesting or that I think people might want to know to make their decision.
Both the roller and the side sweeper brushes are meant to prevent hair from getting wound around them. So far I have found this to be true. You can see the videos the manufacturer has provided that show how this works, so I won’t bother to explain it, just point out that I’ve found it does work. I haven’t had to cut any hair out of the roller since I’ve gotten it, and that was previously a weekly thing.
It does not self empty after vacuuming. However, it compresses the hair and dirt to take up less space. So while I was emptying my big self-emptying canister (on my Shark) about once a month before, I’ve only actually emptied this smaller dust bin once every one to two weeks. Initially, I was worried that this meant it wasn’t picking up as much debris and hair, but upon review, I discovered the compression factor they describe. You also have the option of using dust collection bags that you can take out and dispose of. To be more environmentally friendly, I have not chosen that option, but for people that may have issues with dust, I’m glad it is an option.
It self-cleans and dries the mop pads quite well. I did pick up replacements so that I could wash them, but I’ve needed to wash them with far less frequency than I expected. When I do pull them off to wash, I don’t notice any odor like I was expecting.
The mop water is used to both clean the floor and wash the mop pads. I think the majority of the water usage is to clean the pads, however. So you will need to refill that every 2 to 3 mops probably depending on how big your house is. You can use it with or without Narwal cleaner - do not use other cleaners, or you risk the device dying or messing up your floors due to over foaming.
My one tip is to use any leftover mop cleaner you may have from your regular mopping, and add a capful into the dirty water bin after you empty and rinse it each time. Because if you do not empty that out every day, the stagnant water WILL be stinky. A little scented floor cleaner has seemed to improve that dramatically for me.
Is it expensive? Yes. Is it worth it? RESOUNDINGLY yes. I no longer have to kill myself to have clean floors. And that is the best gift to give myself.
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Reviewed in the United States on December 15, 2025
★★★★★ 5
Amazing robot helper
Color: White
I’ve been using this robot vacuum for about a month now, and I have no regrets about buying it! It’s noticeably quieter than my previous robot vacuum, and the mopping performance is excellent. I really like that it has two large water tanks—one for clean water and one for dirty—which makes a big difference as it easily covers my entire house on a single run.
I don’t have carpet, so I can’t speak to how it performs there, but for vacuuming and mopping tile and hard floors, it absolutely deserves high praise. The battery is another big plus—it can clean my whole house before needing to return to the base to recharge.
The app is fantastic. The mapping is very accurate, and there are plenty of cleaning options to customize different areas of the house. The initial app setup did require some help, but customer support was quick and very helpful. Overall, I’m a very satisfied customer and would definitely recommend it!
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Reviewed in the United States on December 31, 2025
★★★★★ 5
Very great and durable option.
Style: for Saros 10R / Saros 20 / Qrevo CurvX
These parts are the perfect option when maintaining your robot vacuum. They fit the machine perfectly and are just as if not more durable than the company provided parts. Definitely recommended.
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Reviewed in the United States on March 28, 2026