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Description
DPD ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect specimens using EDTA or heparin as anticoagulants and centrifuge them at 1000×g for 15 minutes at 2-8℃ within 30 minutes of collection. The supernatant can be tested or stored at -20℃ or -80℃, but repeated freezing and thawing should be avoided. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 200 ng/mL). Then dilute to the following concentrations: 200 ng/mL, 100 ng/mL, 50 ng/mL, 25 ng/mL, 12.5 ng/mL, 6.25 ng/mL, 3.125 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 200ng/mL standard working solution into the first EP tube and mix thoroughly to make a 100ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotin-Antibody Working Solution: Centrifuge the concentrated Biotin-Antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated Biotin-Antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent) and use on the same day. 4. Preparation of Enzyme Conjugate Working Solution: 15 minutes before use, centrifuge the 100x concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100x concentrated HRP enzyme conjugate to a 1x working concentration with universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Use the same day. 5. Preparation of 1x Wash Solution: Dispense 10mL of 20x Wash Solution into 190mL of distilled water (Concentrated Wash Solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return them to 4°C. 2. Sample Addition: Add 50 μL of sample or standard of varying concentrations to the corresponding wells. Add 50 μL of universal diluent to the blank wells, followed by 50 μL of Biotin-Antibody Working Solution to each well. Cover with film and incubate at 37°C for 1 hour. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate to minimize matrix effects. The sample concentration should be multiplied by the dilution factor when calculating the final concentration. It is recommended to run replicates for all samples and standards.) 3. Plate Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, then shake off the wash solution and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 4. Enzyme Conjugate Working Solution: Add 100 μL of enzyme conjugate working solution to each well. Cover with film and incubate at 37°C for 30 minutes. 5. Wash: Discard the liquid and wash the plate five times as in step 3. 6. Add substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 7. Add stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculation of experimental results: Result evaluation: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction value. Plot a standard curve for the four-parameter logistic function on double-logarithmic graph paper with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a competitive enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled antibody, and HRP conjugate are sequentially added to microwells pre-coated with a universal deoxypyridinol/deoxypyridinoline (DPD) antigen. After incubation and washing, the assay is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the universal deoxypyridinol/deoxypyridinoline (DPD) content in the sample. The absorbance (OD value) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | All | |||||||||||||||||||||||||||||||||
| Synonym | General Deoxypyridinol;DeoxypyridinolineELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Competition Law | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Deoxypyridinoline (DPD), also known as D-pyrilinks, Pyrilinks-D, or deoxyPYD, is one of two pyridine crosslinks that provide structural rigidity to type I collagen in bone. It is measured in urine tests and used along with other bone markers such as alkaline phosphatase, osteocalcin, and N-terminal telopeptide to diagnose bone diseases such as postmenopausal osteoporosis, bone metastases, and Paget's disease. It is also useful in monitoring treatment with bone-active medications such as estrogens and bisphosphonates. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 3.12-200ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, and other biological fluids |
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4.8 ★★★★★
Based on 25 reviews
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Product Reviews
★★★★★ 5
Works well
Size: 3 Fl Oz (Pack of 2), Style: SPF 30
Good product
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 29, 2026
★★★★★ 5
Good sunblock. New bottle design with easy to read expiration date.
Size: 8 Fl Oz (Pack of 1), Style: SPF 30
When reviewing this product. It ask you to put a STAR for:
Sun protection
Water resistance
I put 5 stars because, how would i know? I don't use it to go into water and you'd need to be a scientist testing how good the sun protection is. I just assume it works.
But i've used banana boat sunblock in the past. And it's been fine. No sunburns. As long as you remember to re-apply it because it's not meant to last all day.
Pros:
- It's sunblock
- It works (from my previous experience)
- New bottle design.
They changed the style of the bottle. The new bottle has the date on it. The old bottles only had a serial number. In order to find out if your old bottles are expired you had to go to the banana boat website and see the instructions on how to read the serial # that way you can know the expiration date.
The new bottle is more simple, the date is laid out easy to read on the bottle.
- Long expiration date:
I bought this sunblock around april or may of 2020 and the bottle says it will expire January 01, 2022
- Nice slim bottle to fit into a small bag.
Cons:
- It's a small 8 FL OZ bottle. My old bottle is a 10 FL OZ. Not sure if they still sell the 10FL OZ.
- It feels a little heavy and thick on the skin. But after a while it's fine.
- If you rub your hands / fingers around your eye, especially if you're sweaty. You might get the sunblock into your eyes. That has happened to me on hot summer days. It will make you teary eyed and irritate your eyes for a long while.
- I hear this sunblock may stain clothes. So far that has not happened to me.
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Reviewed in the United States on May 24, 2020
★★★★★ 5
Ideal Sunscreen for Backpacking Adventures!
Size: 3 Fl Oz (Pack of 2), Style: SPF 30
I recently used Banana Boat Sport Ultra Sunscreen Lotion SPF 30 on a backpacking trip, and it was absolutely perfect for my needs. This sunscreen provided excellent protection and convenience, making it a standout choice for outdoor activities.
First off, the SPF 30 rating offers reliable protection against harmful UV rays, which is crucial for long hours spent in the sun. The lotion goes on smoothly and absorbs quickly without leaving a greasy residue, which is a huge plus when you’re on the move.
The size of the bottle is spot-on for backpacking. It’s compact and lightweight, fitting easily into my gear without taking up too much space. This makes it convenient to carry and apply whenever needed, ensuring that I stay protected without adding bulk to my pack.
The water-resistant formula held up well even during sweaty hikes and water crossings. I didn’t have to reapply excessively, and it stayed effective throughout the day. The lotion also has a pleasant, non-overpowering scent, which is a nice bonus.
In summary, Banana Boat Sport Ultra Sunscreen Lotion SPF 30 is an excellent choice for anyone heading into the great outdoors. Its effective sun protection, ideal size for backpacking, and water-resistant properties make it a must-have for any adventure. Highly recommended for keeping your skin safe and comfortable while exploring!
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Reviewed in the United States on September 7, 2024
★★★★★ 5
Good stuff
Size: 3 Fl Oz (Pack of 2), Style: SPF 30
Banana Boat still smells good and works good too
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 20, 2026
★★★★★ 5
Great sunblock, moisturizer, non-sticky and smells great!!
I’ve used a lot of sunscreen in my life being a fair skinned redhead and this product keeps me moisturized and prevented me from getting burned. We recently went to the French Polynesian islands and the sun hits hard down there and I never burned during our trip. I also absolutely loved how my skin felt moisturized and I never had dry skin from being in the pools and in the ocean. Plus, it smells amazing and you don’t feel sticky once it’s on.😊. Would highly recommend. Wish it was a little less in cost, but I love it.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on October 3, 2025