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Description
Rat ARNTL ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Tissue homogenates and other biological fluids Preparation before testing: 1. Please take out the test kit from the refrigerator 10 minutes in advance and
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Tissue homogenates and other biological fluids Preparation before testing: 1. Please take out the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare a gradient standard working solution: Add 1 mL of universal diluent to the lyophilized standard. Let stand for 15 minutes to completely dissolve, then gently mix (concentration 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 10 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with an aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of aryl hydrocarbon receptor nuclear translocator-like protein 1 (ARNTL) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Aryl hydrocarbon receptor nuclear translocator-like protein 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Aryl hydrocarbon receptor nuclear translocator-like protein, also known as ARNTL or brain and muscle ARNT-like protein 1 (BMAL1), is a protein encoded by the BMAL1 gene. BMAL1 encodes a transcription factor with a basic helix-loop-helix (bHLH) and two PAS domains. This 62-amino acid protein plays a key role as one of the positive elements in the mammalian autoregulatory transcription-translation negative feedback loop (TTFL), which is responsible for generating molecular circadian rhythms. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissuehomogenatesandotherbiologicalfluids |
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4.9 ★★★★★
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Product Reviews
★★★★★ 5
The body wash I reach for every time and my skin has never felt better
Scent: Fragranced, Size: 30.6 Fl Oz (Pack of 1)
I have been through a lot of body washes over the years and this Dove Deep Moisture is the one I keep coming back to. The texture is thick and creamy, a little goes a long way, and it rinses clean without leaving any heavy residue behind. After getting out of the shower my skin genuinely feels softer and not dry or tight the way it does with some other washes.
The scent is pleasant and mild, something like a clean, soft powder rather than an artificial floral or chemical smell. It does not linger too long which I actually prefer since heavy fragrances can be irritating. The formula with no sulfates and no parabens makes it a gentler option and I have noticed fewer issues with dry patches since switching to it.
The 30.6 oz size is great for the price and lasts a good while even with daily use. This is just a reliable, high quality everyday body wash that does exactly what it promises. Simple and effective.
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Reviewed in the United States on May 5, 2026
★★★★★ 5
Having a smooth feel
Scent: Fragranced, Size: 30.6 Fl Oz (Pack of 1)
It's thick, creamy and has no perfume. I shower all the time and I usually don't use a soap..I will buy again for sure..It left my skin very moist and not itchy..
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Reviewed in the United States on April 11, 2026
★★★★★ 5
Perfect for Sensitive Skin – Gentle, Moisturizing and Long-Lasting
Scent: Fragranced, Size: 30.6 Fl Oz (Pack of 1)
If I could give it 10 stars, I would!
This Dove Deep Moisture Body Wash is absolutely amazing and hands down my favorite Dove body wash so far. The size is large, so it lasts a long time and offers excellent value for the price, especially considering the quality.
The texture is thick and creamy, not watery at all. It spreads very well on the skin and a little goes a long way. The scent is very pleasant, soft, and not overpowering—it actually reminds me of baby powder, which I personally love. It feels clean and comforting, not artificial or strong.
While showering, it feels gentle and luxurious, and after rinsing, my skin feels soft, smooth, and well-hydrated, never dry or tight. This is especially important for me because I have sensitive skin, and this body wash has been perfect.
Dove is an excellent brand, and I’ve tried several of their body washes over the years. This one was also recommended to me by both a dermatologist and a gynecologist, which gave me even more confidence in using it regularly.
Overall, this is an outstanding product. I highly recommend it, especially for anyone with sensitive skin. If you’re looking for a moisturizing, gentle, and high-quality body wash, this one is a must-try.
💙 Super recommended!
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Reviewed in the United States on January 21, 2026
★★★★★ 4
Great moisturizing body wash, but the scent takes some getting used to
Scent: Fragranced, Size: 30.6 Fl Oz (Pack of 1)
I've been using Dove products for years and this body wash does not disappoint when it comes to moisturizing. My skin feels genuinely soft and hydrated after every shower without that tight, dry feeling I get with other cleansers. The no sulfates and no parabens formula is a big plus for me as I've been trying to be more conscious about what goes on my skin. At 30.6 oz it's also a great size that lasts a good while. My only real note is that the scent is a bit different than what I expected — it's not unpleasant but there's a slightly unusual undertone that I couldn't quite put my finger on at first. It took me maybe a week or so of using it before I stopped noticing it. If you're sensitive to scents it might throw you off initially, but it does fade quickly once you're out of the shower. Overall a solid body wash that delivers on the moisture promise — just go in knowing the scent might need a little adjustment period.
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Reviewed in the United States on May 10, 2026
★★★★★ 5
Wow, Dove really outdid themselves. Great product.
Scent: Fragranced, Size: 30.6 Fl Oz (Pack of 1)
Wow, what a difference what a difference Amazon basic body wash is what I was using before to be honest and it used to be very good. I don’t know if they changed the formula but that formula now it doesn’t lather up. You have to use half the bottle to get it to lather up this one lathers up amazingly well and you only need a few pushes. As a guy, I love it. We have dry skin too, just like anybody else so between moisturizer and lather , the scent the skin protection the skin softeners the whole thing it’s great. And you get a lot of of it too, which is awesome so overall at least to me a great value for the money
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Reviewed in the United States on January 14, 2026
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