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Description
Human GDF9 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Growth Differentiation Factor 9 (GDF9) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Growth Differentiation Factor 9 (GDF9) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Growth Differentiation Factor 9 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Growth differentiation factor 9 (GDF9) is an oocyte-derived growth factor in the transforming growth factor β (TGF-β) superfamily. It is highly expressed in oocytes and has a critical influence on surrounding somatic cells, particularly granulosa cells, accumbens cells, and corpus luteum cells. It exerts its effects through two receptors surrounding the oocyte: it binds to bone morphogenetic protein receptor 2 (BMPRII) and downstream of this, utilizes TGF-β receptor type 1 (ALK5). GDF9 is crucial for the entire process of folliculogenesis, oogenesis, and ovulation, and therefore plays a vital role in female fertility. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.2 ★★★★★
Based on 18 reviews
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Product Reviews
★★★★★ 5
Great Protection
Color: Orange, Size: 13-inch for New MacBook Air & Pro
This is a very nice, protective case. The material is thick and cushions the laptop great! Computer fits nice and cozy; great fit. I love the bright orange! I highly recommend.
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Reviewed in the United States on April 23, 2026
★★★★★ 5
A sold, attractive, case.
Color: Navy Blue, Size: 13-inch for New MacBook Air & Pro
This case looks great, has a nice, lively color scheme, and seems to be reasonably protective and sturdy. I wouldn’t toss a laptop around with only this as protection, but I feel fairly confident in it for small drops. The fabric is easy to clean and has held up well. You can charge without taking it out of the case thanks to a zipper, and it doesn’t add much bulk, making it quite portable. All in all a good balance of protection, cost, and style.
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Reviewed in the United States on June 2, 2026
★★★★★ 5
I would rescue it from a fire even if it were empty. I'm kidding but I do like this sleeve a lot.
Color: Orange, Size: 13-inch for New MacBook Air & Pro
Fits perfectly. Superb padding. The corner padding inspires confidence-not that I want to test it! The orange color is-chef's kiss-beautiful. A perfect contrast to the midnight color of my MacBook. I could gush about that stuff all day. But you all want to know about the other stuff.
Zippers: Two YKK no snag bi-directional closers for the main opening and a solo no snag YKK for the side pocket.
Outside: Feels like tactical nylon type fabric. I haven't and won't intentionally test its stain resistance. But I suspect it would clean up just fine.
Interior: A very soft microfiber like interior covering an odor free padding that is firm yet forgiving. There is a recess you push your laptop into making for a snug fit that, like I wrote earlier, inspires confidence. This sleeve is larger than some competing brands but seeing the recess in person explains why.
Having touched on the size of this sleeve, it maintains the svelteness of my MacBook Air. Yes, it is thicker than some other sleeves, by a smidge. However the additional protection suggested but that smidge, that minute extra ish is worth it to me. The sleeve fits in my travel backpack, so that's all good.
Lastly, I would consider putting a tablet or phone in the sidepocket but not anything that would concentrate the any energy that dissipates during a fall. Had I designed this product I wouldn't have included the side pocket. It's great for documents and thin things but the sell page suggests chargers. That seems like inviting risk. You do you.
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Reviewed in the United States on January 22, 2026
★★★★★ 4
Better Lips, Better Fit, Less Nonsense
Color: Black, Size: 13-inch for New MacBook Air & Pro
I tested this sleeve with a 13.6” MacBook Air (M4), and the difference is immediately noticeable.
First thing: fit. This one actually holds the laptop in place. The padded “lips” in the corners create just enough resistance that you have to guide the MacBook in and out. It’s not a loose drop-in like other cases — it feels controlled. If I shake it, there’s barely any movement, which is exactly what I was looking for.
Second: build quality. The stitching is clean and consistent — almost surgical compared to the wrinkled mess I saw on another model. The outer fabric stays flat, no weird waves or cheap-looking folds. The interior lining is softer and thicker too, so it feels more protective without being bulky.
Third: structure. I even did a quick “real-world” test — stood it vertically on a mattress, zipper open, applied pressure from the top — and it held its shape. The other case I tried would have collapsed immediately. This one actually feels like it can handle normal use without transferring pressure straight into the laptop.
The external pocket is simple but practical. It fits the charger brick and cable without interfering with the main compartment, which keeps the laptop fit intact.
Only downside: I could do without the orange accents. A full black version would be perfect. But honestly, that’s cosmetic — functionally, this one does what it’s supposed to do.
At around this price point, this feels fair. Not luxury, not overhyped — just a well-executed, practical sleeve that gets the fundamentals right.
Bottom line: not perfect, but finally something that feels properly made and actually holds the laptop instead of just surrounding it.
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Reviewed in the United States on March 29, 2026
★★★★★ 5
Great Laptop Case !
Color: Black, Size: 13-inch for New MacBook Air & Pro
This case is reallllly nice and durable, the cushioned corners is what really makes this case special imo. The outer layer is a nice durable think fabric. It fits perfectly. The only downside is the zipper traction is not as smooth as I would like ,,, but I guess I can fix that with some oil/wd40. I would recommend this laptop case ! 👌🏽👌🏽👌🏽
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Reviewed in the United States on March 24, 2026
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