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Description
Mouse ADAM19 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be ultrasonically disrupted or repeatedly frozen and thawed. Finally, the homogenate is centrifuged at 5000×g for 5-10 minutes and the supernatant is collected for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against A disintegrin and metalloprotease 19 (ADAM19). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of A disintegrin and metalloprotease 19 (ADAM19) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse A Disintegrin and Metalloprotease 19 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | disintegrin and metalloproteinase 19 (MADDAM, meltrin beta) is a member of the ADAM (A disintegrin and metalloprotease domain) family. Members of this family are membrane-anchored proteins structurally related to snake venom degrading proteins and have been implicated in various biological processes involving cell-cell and cell-matrix interactions, including fertilization, muscle development, and neurogenesis. This member is a type I transmembrane protein and serves as a marker for dendritic cell differentiation. It has also been shown to be an active metalloprotease and may be involved in normal physiological and pathological processes, such as cell migration, cell adhesion, cell-cell and cell-matrix interactions, and signal transduction. Alternative splicing results in two transcript variants. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates and other biological fluids |
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4.2 ★★★★★
Based on 464 reviews
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Product Reviews
★★★★★ 4
Sam + Percy 💕
Format: Kindle
Every Summer After is a perfect summer read and although it had been in my TBR for so long, I really enjoyed it. I’m a sucker for second chance romance and childhood friends to lovers and I was captivated immediately. Their friendship blossomed into a cute young love romance until one impulsive decision during a vulnerable moment lead to the downfall of Sam & Percy’s relationship.
Now over a decade later, Percy makes the trip back to the lake to attend a funeral and memories flood back of her summers with Sam. But does time heal all wounds? Read to find out.
I really love young love romances because they are innocent with heighten emotions, personal growths and can be extremely messy. Not to mention the angst that keeps me wanting to read more and this book definitely kept me engaged.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on September 7, 2025
★★★★★ 5
Warm Blanket Read
Format: Paperback
Wow, I didn’t know how much I needed this and I inhaled it. I needed to feel a bit of summer. I needed a small town. What I didn’t know I needed was a big love story. This was perfect and escapist. A perfect read to make you feel good and characters to swoon over. Can’t wait for the show now.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 30, 2026
★★★★★ 5
Heartfelt and Nostalgic
Format: Paperback
What can I say to effectively capture the beauty, sweetness, and depth of this story?
Sam and Percy’s story is sweet and nostalgic, and it made me feel like I was a teenager again right along with them. It brought back memories of my own teenage years spent camping at various lakes with family and friends; memories I hadn’t thought about in awhile.
Barry’s Bay is a character in its own right as well, and I had such a vivid picture in my mind of what it must have looked like.
There were moments when I laughed out loud, and moments that made me cry. The exploration of guilt and what it does to a person and the ones they love was so emotional and real.
And the sweetness and love between Percy and Sam, despite all their mistakes and miscommunications, is something that will stick with me for a long time.
There is so much heart in this story. I love a good friends-to-lovers romance, and have read quite a few, but this is easily one of the best.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on April 10, 2026
★★★★★ 4
Cute little read
Format: Paperback
I thought this book was a good quick read!
Plot: 4⭐️ I won’t spoil it but there is a trope I wasn’t fond of in this toward the end of the book, BUT it’s a very realistic response to the situation. (I am not making it okay in ANY means). I do wish there would have been a little more fun memories between them as friends.
Characters: I think Carly did a great job keeping the characters realistic. Yes the FMC parents had some money but it was a refresher that nobody was spoiled and the MMC earned his way into medical school.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 20, 2026
★★★★★ 5
John Candy References!
Format: Kindle
Persephone is thirty years old and she just got dumped. She didn’t really care, he didn’t mean much to her and she never really let him in, even after seven months. She gets a phone call that has her racing back to her past, the small town of Barry’s Bay, and back into the life of the one who got away… Sam Florek.
For six summers Percy and Sam were inseparable. The friendship naturally blossomed and grew into something more and he became the man she never wanted to live without, until it fell apart in a splash.
Percy returns for Sam’s mothers funeral and all the feelings come rushing back, their connection burning as brightly as it had twelve years ago. But Percy has a secret, a reason for why she broke up with him, and until she tells him, they can never be together.
Told in dual timelines, we unravel the mystery of the once in a lifetime love story between two people who were as fated as a couple could be.
First and foremost, there are not one but TWO John Candy references in this book. He is a late Canadian actor who was popular in the 80s. Most will know him as the Polka Player from home alone but I know him from so much more. Trust me, he deserves this paragraph of recognition and I praise the author for including him as he is slowly being forgotten.
Now onto the story.
I loved it. No ifs ands or buts about it. I absolutely love this story. It is a dual timeline, second chance romance and I think it got me out of my reading slump. I finished it in less than 2 days and couldn’t put it down.
The history of the characters, watching them slowly fall in love and come to terms with their feelings. Seeing how they struggle over the long distances and other romances that pop up. It was so well written and it felt like I was watching two people I knew in real life fall for each other. It was so realistic , even the lows had me tearing up but remaining hopeful.
The twist, the reason why they broke up, was predictable. I saw it coming not long into the book but it didn’t take away from the heartache I felt when it was revealed.
I enjoyed that we also got to explore relationships outside of the main love interest. We got to know Percy’s friends and how they interacted with Sam too which was a nice break from the romance and gave depth outside of the love interest.
Great book and I’m so thankful it got recommended to me.
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Reviewed in the United States on March 24, 2023
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