Mouse TNF-α ELISA Kit(Plus)

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti mouse tnf-&alpha  Capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process; Tnf-&alpha  Bound to solid-phase antibody and detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response and sample   Tnf-α There is a positive correlation between the concentration of. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: Pre coated 96 well plates, standards, tnf-α One copy of detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.99 pg/ml

Detection Range: 10.94-700pg/ml

Recovery Range: 81-119%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Tumor necrosis factor alpha; (tnf-α)Also known as cachexia and tnfsf1a, it is an adipokine that participates in systemic inflammation and is also one of the cytokines that stimulate the acute phase response. It is mainly produced by activated macrophages, but also secreted by other types of cells, such as cd4+ lymphocytes, NK cells, neutrophils, mast cells, eosinophils and neurons. It has a variety of regulatory functions in the immune response and can also serve as a potential pyrogen. Tnf-α After responding to stimuli (infectious agents or tissue damage), it activates neutrophils in the systemic circulation, changes the characteristics of vascular endothelial cells, regulates the metabolic activity of other tissues, and exhibits tumor killing activity by inducing local coagulation. Tnf-α It plays an important role in the pathogenesis of inflammation in joint tissues and other tissues. Recently, more and more information shows that tnf-α It is also involved in the pathogenesis of AIDS. Tnf-α The assay is also very useful for transplantation research.