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Description
UniOne® TR-FRET Human IL2/IL2R Binding KitProduct Specification Host Human Stability & Storage 80 Background This kit utilizes HomogeneousTime Resolved Fluorescence (TR FRET) technology to measure the interactionbetween IL2 and IL2R. This method enables simple and rapid high throughputscreening of inhibitors and blocking antibodies. As shown, the interaction betweenIL2 and IL2R is detected using a Eu labeled Anti Tag1 Antibody (TR FRET donor)and an Acceptor labeled Anti Tag2 Antibody (TR FRET
Product Specification
| Host | Human |
| Stability & Storage | -80℃ |
Background
This kit utilizes HomogeneousTime-Resolved Fluorescence (TR-FRET) technology to measure the interactionbetween IL2 and IL2R. This method enables simple and rapid high-throughputscreening of inhibitors and blocking antibodies.
As shown, the interaction betweenIL2 and IL2R is detected using a Eu-labeled Anti-Tag1 Antibody (TR-FRET donor)and an Acceptor-labeled Anti-Tag2 Antibody (TR-FRET acceptor). The binding ofIL2 to IL2R brings the donor and acceptor antibodies into proximity. Excitationof the donor antibody triggers Fluorescence Resonance Energy Transfer (FRET) tothe acceptor antibody, resulting in a specific emission signal at 665nm. Thisspecific signal is proportional to the degree of interaction between IL2 andIL2R. This homogeneous assay is simple to perform and requires no washingsteps.
Components
Component |
Concentration |
100 TESTS |
500 TESTS |
2500 TESTS |
10000 TESTS |
Storage Temp. |
|---|---|---|---|---|---|---|
Tag1-IL2 protein |
40× |
10μL |
50μL |
250μL |
1000μL |
-80°C |
Tag2-IL2R protein |
100× |
4μL |
20μL |
100μL |
400μL |
-80°C |
Basiliximab |
100× |
3μL |
10μL |
50μL |
200μL |
-80°C |
Anti-Tag1 Eu dye antibody |
50× |
10μL |
50μL |
250μL |
1000μL |
-80°C |
Anti-Tag2 Ac antibody |
12.5× |
40μL |
200μL |
1000μL |
4000μL |
-80°C |
Detection buffer |
10× |
400μL |
2mL |
10mL |
40mL |
-80°C |
*Repeated freeze-thaw cycles should be avoided. Aliquot upon first thaw and store according to the specified conditions. Valid for one year.
Shipping Notes
- Free Standard Shipping on $100+ Orders to the USA.
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
- To process your return/exchange, please contact us at [email protected]
- Please click here for more details>>> Return & Exchange Policy