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Description
Rat PER2 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant and centrifuge at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. Cell Supernatant: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse the tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. Preparation before testing: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the gradient working solution of the standard: Add 1 mL of universal diluent to the lyophilized standard. Let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a Period Circadian Protein 2 (PER2) capture antibody. After incubation and washing, the assay is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the amount of Period Circadian Protein 2 (PER2) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Period Circadian Protein 2 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | PER2 is a protein encoded by the PER2 gene in mammals. PER2 has attracted attention for its central role in circadian rhythms. Disorders associated with PER2 include advanced sleep phase syndrome, familial 1, and advanced sleep phase syndrome. Pathways associated with PER2 include the metabolism and effects of the circadian clock and melatonin. Gene ontology annotations associated with this gene include transcription factor binding and transcription cofactor activity. An important homologue of this gene is PER1. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.312-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, cell supernatant, tissue homogenate, etc. |
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4.8 ★★★★★
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Product Reviews
★★★★★ 5
Brown skin approved!
Size: 6 Ounce (Pack of 1), Size: 6 Ounce (Pack of 1)
Does not leave a white cast, feels very light on the skin. Scanned on Yuka and was a 86/100 green zone! It’s so hard to find a sunscreen that isn’t trying to poison you, scanned all the sunscreen at Walmart and were rated a 1/100 through 6/100. Which brought me to Amazon. Thinksport has my vote on goodness and healthiness, would buy again!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 29, 2026
★★★★★ 5
My favorite... And I'm picky.
Size: 3 Ounce (Pack of 1)
I've become a little obsessed with sunscreen over the last past 5 years. I have olive skin and developed melasma after my first pregnancy. I learned the hard way that chemical sunscreens don't help if you have melasma - and they actually make the dark spots even worse. Also the active ingredients in chemical sunscreens (like Oxybenzone and Octinoxate) are absorbed by your skin, and may cause allergies, hormonal imbalance and be toxic overtime. After learning that, I started wearing only mineral sunscreen (active ingredient is zinc and/or titanium dioxide). These are not absorbed and works by creating a physical barrier that reflects the sun (some studies suggest that some nanoparticles of zinc may be absorbed by your skin, so to be really, really safe you should look for non-nano particles).
After trying a few brands, I realized the sunscreens with only zinc in their formulas worked better than the blends with titanium dioxide, but this is just my personal preference I guess. And the higher the percentage of zinc, the better.
Most pure mineral sunscreens out there are 10-ish % zinc, SPF 30, 40 min water resistant - which is ok.
Thinksport goes beyond that: It's 20% non-nano zinc, SPF 50+, 80 min water resistant. It really can't get any better than that!
The lotion is not extremely thick when compared to other sunscreens I've tried and I find it pretty easy to apply. As every zinc sunscreen, it does leave a little fine white cast, but honestly Thinksport is not bad at all! It smells nice, lightly citrusy - and the smell vanishes soon. And, it's not overpriced.
Best I've tried so far.
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Reviewed in the United States on April 15, 2016
★★★★★ 3
Works but will Dry Out Your Skin
Size: 6 Ounce (Pack of 1)
I am a swim instructor at an outdoor pool and this stuff DOES work. I can apply it at the beginning of a 5 hour shift and while I have experienced light tanning it has protected against burning completely without reapplication.
The white cast is significant though I don't mind it aesthetically, I know its there for protection . It does rub off on things though so consider yourself warned.
Major Con: it is incredibly drying, and the soap needed to scrub it off is A LOT. That coupled with chlorine is a lot for my skin . Still on my sunscreen journey.
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Reviewed in the United States on May 4, 2026
★★★★★ 5
This book was amazing!
Format: Kindle
I absolutely loved this book! Avery and Knox were beyond perfect for each other. Rebecca Jenshak did such an incredible writing this book and these characters. The brothers relationships in this family is wonderful. Everyone steps up to help take care of each other and it is heart warming. This book had low angst which I loved! I am really enjoying the start to this series and I cant wait to read the next book!
The duet audiobook was incredible! Erin Mallon and Teddy Hamiliton did a fantastic job bringing this book and these characters to life! I highly recommend this book, audiobook, and series!
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Reviewed in the United States on April 22, 2026
★★★★★ 4
Banter, a bit of angst, found family, and a hot, tattooed bad boy who’s really a totally softie
Format: Kindle
This was a fun, easy read. It has all the things I love in a sports romance...banter, a bit of angst, found family, and a hot, tattooed bad boy who’s really a totally softie.
Knox is kind of a mess at the start. He’s super talented, but has a reputation problem after getting kicked off his motocross team. He’s desperate for a second chance, which leads him to Avery, a former Olympic gymnast recovering from an injury. She’s trying to get her confidence back, and somehow these two end up helping each other.
They can't stand each other at the beginning. He says ALL. THE. WRONG. THINGS. Evenutally, their friendship builds into something more. It felt natural and sweet. I liked that they both had their own stuff going on. Avery’s inner struggle was really relatable, and Knox’s bond with his brothers, especially with Flynn, added a ton of heart to the story.
The beginning was little slow, and the ending felt a little rushed. Overall, though? I had a great time with this book.
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Reviewed in the United States on July 27, 2025
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