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Description
G4S Linker Recombinant Rabbit mAb (Alexa Fluor® 647 Conjugate) (S-711-23)Product Specification Host Rabbit Immunogen Synthetic Peptide Clone Number S 711 23 Antibody Type Rabbit mAb Isotype lgG Rabbit F(c) Application FCM Reactivity Species Independent Purification Protein A Research Area CAR T Concentration 10g ml Conjugation Alexa Fluor 647 Physical Appearance Liquid Storage Buffer PBS, 25% Glycerol, 1% BSA, 0. 3% Proclin 300 Stability & Storage 12 months from date of receipt reconstitution, 2 to 8 C as supplied.
Product Specification
| Host | Rabbit |
| Immunogen | Synthetic Peptide |
| Clone Number | S-711-23 |
| Antibody Type | Rabbit mAb |
| Isotype | lgG/Rabbit F(c) |
| Application | FCM |
| Reactivity | Species Independent |
| Purification | Protein A |
| Research Area | CAR-T |
| Concentration | 10μg/ml |
| Conjugation | Alexa Fluor® 647 |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 25% Glycerol, 1% BSA, 0.3% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied. |
Dilution
| application | dilution | species |
| FCM | 1 μl per million cells in 100μl volume | Species independent |
Background
G4S Linker is a commonly used flexible amino acid linker that plays a significant role in antibody expression and design. The sequence of this linker is (Gly-Gly-Gly-Gly-Ser)n, where "n" represents the number of repetitions. By altering the value of "n," the length of the GS amino acid chain can be optimized to achieve appropriate separation of antibody or protein domain functions or to maintain necessary inter-domain interactions. G4S Linker is typically used in single-chain variable fragments (scFv) and chimeric antigen receptors (CARs) to connect the variable heavy (VH) and variable light (VL) domains for the recognition of target antigens. The length of this linker is crucial for controlling the stability of scFv and the non-covalent association between the VH and VL domains. In CAR structures, the G4S Linker is located in the extracellular domain of the scFv, helping to maintain the structure and function of the CAR. Additionally, G4S Linker is used to detect CAR cells designed with G4S Linker, which offers high specificity and sensitivity.
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