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Description
Neurofilament H Recombinant Rabbit mAb (S-532-103)Product Specification Host Rabbit Antigen Neurofilament H Synonyms Neurofilament heavy polypeptide; NF H, 200 kDa neurofilament protein; Neurofilament triplet H protein; NEFH; KIAA0845 Immunogen Recombinant Protein Location Cytoplasm, Cytoskeleton, Cell projection Accession P12036 Clone Number S 532 103 Antibody Type Recombinant mAb Isotype IgG Application WB, IHC P, IF Reactivity Hu, Ms, Rt Purification Protein A Concentration 0. 5 mg ml Conjugation
Product Specification
| Host | Rabbit |
| Antigen | Neurofilament H |
| Synonyms | Neurofilament heavy polypeptide; NF-H, 200 kDa neurofilament protein; Neurofilament triplet H protein; NEFH; KIAA0845 |
| Immunogen | Recombinant Protein |
| Location | Cytoplasm, Cytoskeleton, Cell projection |
| Accession | P12036 |
| Clone Number | S-532-103 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, IF |
| Reactivity | Hu, Ms, Rt |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05%BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | |
| IHC-P | 1:500 | |
| IF | 1:500 |
Background
Neurofilaments (NF) are classed as type IV intermediate filaments found in the cytoplasm of neurons. They are protein polymers measuring 10 nm in diameter and many micrometers in length. Together with microtubules (~25 nm) and microfilaments (7 nm), they form the neuronal cytoskeleton. The protein composition of neurofilaments varies widely across different animal phyla. Mammalian neurofilaments are heteropolymers of up to five different proteins: NF-L, NF-M, NF-H, α-internexin and peripherin. The precise composition of neurofilaments in any given nerve cell depends on the relative expression levels of the neurofilament proteins in the cell at that time. For example, NF-H expression is low in developing neurons and increases postnatally in neurons with myelinated axons.[6] In the adult nervous system neurofilaments in small unmyelinated axons contain more peripherin and less NF-H whereas neurofilaments in large myelinated axons contain more NF-H and less peripherin.
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