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Description
Rat CST ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with corticosteroid (CST) capture antibodies. After incubation and washing, the sample is developed using the substrate TMB. TMB converts to blue under the catalysis of HRP and to yellow under the action of acid. The intensity of the color is positively correlated with the corticosteroid (CST) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Rat | |||||||||||||||||||||||||||||||||
| Synonym | Rat Corticosteroid ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Procortistatin is a protein encoded by the CORT gene. Its 105 amino acid residues are cleaved into cortistatin-17 and cortistatin-29. Cortistatin-17 is the only active peptide derived from the procortistatin. Cortistatin is a neuropeptide expressed in inhibitory neurons in the cerebral cortex and shares strong structural similarities with somatostatin. Unlike somatostatin, it enhances slow-wave sleep when injected into the brain. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.7 ★★★★★
Based on 17 reviews
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Product Reviews
★★★★★ 5
LAVAZZA
Flavor Name: Espresso, Size: 35.2 Ounce (Pack of 1)
This is my go-to coffee for a smooth, rich espresso every morning. The medium roast has a perfect balance without any bitter aftertaste. Since they are whole beans, the freshness and aroma are top-notch once ground. It’s a great value for a high-quality 100% Arabica blend!
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Reviewed in the United States on April 22, 2026
★★★★★ 5
The Best Coffee Beans in the World for the Price
Flavor Name: Espresso, Size: 35.2 Ounce (Pack of 1)
First of all, my headline is an opinion. I understand that coffee is beloved by most of humanity and that it engenders strong opinions, so don't be offended if you disagree. Let me state my case and build some background and context:
1. I drink coffee black. If folks are putting cream and sugar into their coffee, or making espresso drinks with milk like lattes or Cappuccinos, I feel as though they don't have to worry quite as much about things like acidity/bitterness in coffee because milk changes the taste a lot. If you also drink coffee black, this review is perfect for you.
2.. This review is meant for people who intend to brew these coffee beans with a classic Drip Coffee Maker or a French Press. This is because these are the only methods I've used to brew the coffee. While these beans from Lavazza are called "Espresso Roast Coffee", that does not mean they can only be used for Espresso. If a coffee roaster labels something an "Espresso Roast", it simply means that roaster believes the beans would do well when prepared as an Espresso. That does not mean it can't also be used via other preparation methods. There is a little picture/symbol on the front of the package that indicates this is also good when prepared as Drip Coffee or via French Press.
2. While this particular "Espresso Roast" is not the only type of coffee beans from Lavazza that can be used for drip coffee, I would recommend sticking to varieties that are labeled "100% arabica." There are two types of coffee beans: Arabica and Robusta. Like anything else, you could Google this and find 2 full pages of info to read regarding the differences between these two bean types. But the easiest breakdown seems to be that Robusta beans are more intense (or robust, guess it's in the name) but often more bitter. The bitterness especially becomes present when the coffee is slowly brewed as drip coffee. This is why Lavazza has lots of varieties so you would need to use their website to determine the full list of products they sell that are 100% arabica. You will see that they only have the icon indicating the coffee is good to be prepared as drip coffee for their 100% arabica blends, going along with what I'm saying.
3. Going back to point 2, this is what makes Lavazza Whole Bean Espresso Roast so amazing. There is simply no bitterness regardless of how strong you make it. When you look at Lavazza's description of tasting notes for this blend, they mention Floral and fruity notes. This is where I always feel dumb as I don't personally get those notes. For me it's extremely rich, full bodied, and I get those nice chocolatey, roasty notes. I don't mean like dark chocolate or deeply roasted notes like I would get from a dark roast, because you can clearly tell it's a medium roast. More of a milk chocolate type of flavor profile. I definitely don't get the fruity/floral notes Lavazza mentions, which for me is good as those are not my preferred flavors. I'm not saying that those listed flavor notes are purely just subjective or matter of opinion, but I taste what I taste.
At the end of the day, in my mind what makes the coffee quality is that even when you brew it much stronger than what you might say is average, there's simply no acidity or bitterness. If you purchase the 2.2 pound bag and drink this coffee for a month straight (or however long the bag lasts) and then go back to major American brands, like ones with initials like SB or DD, the acidity and bitterness will become obvious immediately.
4. Regarding point 3, am I saying there are no American Coffee Brands or American Coffee Roasters that make good coffee that isn't bitter and acidic? No I'm not. What I'm saying is that those will be boutique coffee roasters or smaller brands that will be much more expensive than this Lavazza Coffee. Are there any big American Coffee Brands that make coffee like Lavazza? I'm saying NO. Flat out, unequivocally, no. Lavazza is a billion dollar Italian brand. I love to support local companies when possible, but they are not going to be price competitive with Lavazza. So as your everyday coffee, if price is important to you like it is to most, then obviously it's all about finding the best for the cheapest. Queue this coffee.
5. This coffee should run you no more than $18 full price for 2.2 pounds as of December 2024. You may occasionally see it priced higher, this means it's sold out through Lavazza directly and a third party seller has jacked up the price. I would not purchase the coffee from any other seller than Lavazza so watch out for that. Just as importantly, YOU DO NOT have to pay $18 every time if you watch for sales. There are frequent sales of anywhere from $2-$5. So you can get this for as cheap as $13 which is an absolute steal for 2.2 pounds of this glorious coffee. It's still a great deal at $18 for the quality, but at $13 or $14 it's the ultimate no brainer. Maybe order 2 bags when it's on sale.
6. You don't get to control how fresh the beans are when you receive them which sucks. But to Lavazza's credit, they often arrive within 6 weeks of the packaging date. This is also to their credit: They have the date the beans were packaged on the side, not just the expiration date. The key thing to know is that there is indeed a big difference if you are drinking 4-6 week old beans (never got them fresher than that) vs 3 or 4 month old beans. The difference in freshness is pronounced both in the taste and even more so the smell. This is just the reality of ordering this coffee at this amazing price, have to trust you will get it relatively fresh. For my experience, I have for the most part.
Bottom Line: If you are an American like me mostly making and drinking drip coffee, and maybe not as sophisticated as an Espresso drinker, drinking this Lavazza coffee will make you realize that other brands you have been consuming are acidic and bitter when brewed as drip coffee. You will probably either place this on Autoship if that's your kind of thing, or at the very least you will order it again. But I guess you will have to take the chance on it to find out. I don't think $18 (at most) is that big a risk.
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Reviewed in the United States on December 11, 2024
★★★★★ 5
Smooth, Rich Coffee That Makes Every Morning Better
Flavor Name: Espresso, Size: 35.2 Ounce (Pack of 1)
I have tried so many coffee brands over the years but Lavazza Espresso Whole Bean Coffee has really become one of my favorites. The instant I opened the bag, the smell was wonderful. It smelled like rich, fresh coffee, and my kitchen instantly turned into a cozy little café.
The smoothness and balance of flavor is what I like about this coffee. Some espresso coffees can be too bitter or strong but this one has a very nice medium roast taste that is rich without being overbearing. It tastes slightly sweet and creamy, so it is good both as regular coffee and in espresso drinks.
I run these beans through my espresso machine every morning and they grind beautifully. The crema is thick and a beautiful golden color, which makes my homemade lattes and cappuccinos all the more special. Even my husband who is very particular about coffee said how fresh and flavorful it is.
The 2.2-pound bag is also a great value, as it lasts a long time, even in a house full of coffee drinkers. I like that the beans stay fresh when sealed properly and I have not had any oily/stale beans in the bag.
I also like that this coffee gives you a nice energy boost and doesn't taste harsh or acidic. It's smooth enough that I can drink more than one cup without it upsetting my stomach.
Overall, I think Lavazza Espresso Whole Bean Coffee is a great choice for anyone who loves strong, smooth coffee with a high-end cafe taste at home. I will definitely keep buying this for my daily coffee habit.
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Reviewed in the United States on May 28, 2026
★★★★★ 5
Solid coffee, good intro to Lavazza
Flavor Name: Espresso, Size: 35.2 Ounce (Pack of 1)
I've used this and it's genuinely good coffee. The 100% Arabica beans produce a smooth, clean cup with a nice aroma and solid crema. No bitterness, easy to drink and works well in both espresso and drip.
That said, if you're deciding between this and the Lavazza Barista Gran Crema or Qualita Oro, I'd go with those every time. The flavor is richer, the crema is thicker and the overall experience is just a step above. This one is still a solid 4.5 in my book, rounding up to 5, but the Gran Crema and Oro are my personal go to.
If you're new to Lavazza this is a great starting point. You won't be disappointed.
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Reviewed in the United States on May 28, 2026
★★★★★ 4
⭐️⭐️⭐️⭐️ Great Coffee, but Price Fluctuates a Lot
Flavor Name: Espresso, Size: 35.2 Ounce (Pack of 1)
The Lavazza Espresso whole bean coffee is very good quality. The medium roast is smooth, balanced, and works well for espresso, lattes, and cappuccinos. Beans grind evenly and produce a nice crema.
My only issue is the price instability. Sometimes it’s around $17, which is an excellent value, but other times it jumps to $26, which feels high for the same product. Because of this, it’s best to watch the price and buy when it drops.
Overall, great coffee with consistent flavor — just keep an eye on pricing before purchasing.
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Reviewed in the United States on January 19, 2026
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