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Description
Human BAFFR ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a B-Cell Activation Factor Receptor (BAFFR) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the B-Cell Activation Factor Receptor (BAFFR) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human B-Cell Activation Factor Receptor ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | B-cell activating factor receptor (BAFFR), also known as tumor necrosis factor receptor superfamily member 13C (TNFRSF13C) and BLyS receptor 3 (BR3), is a membrane protein of the TNF receptor superfamily. B-cell activating factor (BAFF) enhances B cell survival in vitro and is a regulator of peripheral B cell populations. The protein encoded by this gene is a receptor for BAFF and contains an extracellular phenylalanine-rich domain. This receptor is the primary receptor required for BAFF-mediated survival of mature B cells. During B cell maturation, only a limited number of B cells survive due to regulation by BAFF-R. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.156-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.8 ★★★★★
Based on 25 reviews
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Product Reviews
★★★★★ 5
To strengthen and add shine.
Scent: Bright Coffee, Size: 4.06 Fl Oz (Pack of 1)
Absolutely love this Batana Oil! My hair feels softer, thicker, and much healthier after just a few uses. It helps with dryness and gives my hair a natural shine without feeling greasy. The scent is nice and the jar lasts a long time because a little goes a long way. Highly recommend for anyone looking to improve hair growth and overall hair health!
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on May 18, 2026
★★★★★ 5
High quality
Scent: Bright Coffee, Size: 4.06 Fl Oz (Pack of 1)
I’ve been very happy with this Batana Oil. It feels like a high-quality, 100% raw and natural product. Since adding it to my hair care routine, my hair has felt softer, more moisturized, and healthier overall. I’ve also noticed less breakage and improved manageability.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on June 2, 2026
★★★★★ 5
Clear Self Tanner, Main Character Results
Color: Ultra Dark, Size: 16 Fl Oz (Pack of 1)
I love, love, LOVEEE b.tan. I discovered them during lockdown in 2020 when we were all chasing serotonin and a decent glow, and I’ve been loyal ever since. The scent, the color, the fact that I never end up orange or streaky… they’ve earned my trust. So when I saw they had a clear gel formula, curiosity won. Immediately.
And yes, it delivered.
Packaging (small detail, big win):
Before we even get to the tan itself, I need to mention the pump clip. The bottle arrived with a little clip around the pump stem so it couldn’t be pressed during shipping. No leaks. No mystery tan explosions. I’ve kept the clip for travel because it’s genius. More brands need to do this.
Gel consistency:
This gel is the perfect middle ground. Not thick and sticky, not watery and messy. It spreads easily and evenly, especially with a tanning mitt, which I always recommend no matter the formula. It glides on smoothly and blends without effort. No panic blending, no racing the clock.
No-rinse, no transfer:
It goes on completely clear, which means no guide color, no stained sheets, and no awkward “don’t touch me” phase. It dries fairly quickly too. I was dry enough in about 10 minutes to throw on loose sweats and go about my evening. Dark, loose clothing is still your friend, but this formula is forgiving.
Color & glow:
This develops fast. Within 20–30 minutes, I could already see color starting to come through. By about 5–6 hours later, I had a deep, sun-kissed bronze that looked natural and even. No streaks. No orange tones. Just a rich glow that looks like you spent time outside instead of with a mitt in your bathroom. On top of all of this it leaves my skin feeling hydrated like I used a body lotion.
Scent:
This is one of the reasons I keep coming back to b.tan. There’s no classic self-tanner smell. It actually smells good, fresh and clean, and that stays true even as the tan develops. No “what is that smell?” moments.
Product usage & value:
What surprised me most is how little product I used compared to foam formulas. A little goes a long way. At $15 for a large bottle, the value is excellent, especially considering how dark and even the results are.
Overall, this gel is everything I want in a self-tanner: easy, clear, fast-developing, great-smelling, and deeply bronzing without drama. Five stars, zero hesitation, and officially my new go-to in the b.tan lineup.
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on January 30, 2026
★★★★★ 5
Sun kissed without the sun damage!
Color: Ultra Dark, Size: 16 Fl Oz (Pack of 1), Color: Ultra Dark, Size: 16 Fl Oz (Pack of 1)
I’m officially obsessed with this self tanner. The glow is unreal! Super natural, bronzy, and not even a hint of orange. It gives that “just got back from vacation” look without the sun damage. It actually smells GOOD while applying, which is rare for self tanners. Typical self tanner smell once it sets.
It goes on clear, which I love because there’s zero transfer onto clothes or sheets. The only downside is that since there’s no guide color, it can be a little tricky to see where you’ve already applied it, so take your time and blend well.
But honestly... The end result is flawless, even, glowy skin. 100% a staple for me now!
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Reviewed in the United States on April 17, 2026
★★★★★ 4
Good lasting color
Color: Ultra Dark, Size: 16 Fl Oz (Pack of 1)
I have been a big fan of this brand for quite a while. I do like this specific one. The smell is not over baring however I always mix any self tanner with lotion or oil to help it go on more evenly as well as masks and possible unappealing smell. It does not rub off on clothes unless your sweating then it may but I refrain from wearing white the first day I apply but after that it’s no problem. Color is good for me. I’m fair skinned so it doesn’t so it works well for me. But the downside to that is it can be more noticeable when it starts to fade because it doesn’t fade evenly. It fades as your skin cells slough off so a good exfoliating shower helps with that. Then reapply! And the color last longer then most other brands I’ve tried
WAS THIS REVIEW HELPFUL?YesReportShare
Reviewed in the United States on March 23, 2026
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