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Description
UniOne® TR-FRET Human CD40/CD40L Binding KitProduct Specification Host Human Stability & Storage Store the kit at 80C or below. When stored properly, the reagents remain stable until the expiration dateprinted on the label. Once thawed, the CD40 and CD40L stock solutions canundergo one additional freeze thaw cycle; the labeled antibody solutions canundergo one additional freeze thaw cycle. To avoid repeated freeze thaw cycles,it is recommended to aliquot the remaining stock solutions into
Product Specification
| Host | Human |
| Stability & Storage |
Store the kit at -80°C or below.When stored properly, the reagents remain stable until the expiration dateprinted on the label. Once thawed, the CD40 and CD40L stock solutions canundergo one additional freeze-thaw cycle; the labeled antibody solutions canundergo one additional freeze-thaw cycle. To avoid repeated freeze-thaw cycles,it is recommended to aliquot the remaining stock solutions into single-usecontainers for storage at -80°C. Thawed dilutions and Detection Buffer can bestored at 2-8°C. |
Background
This kit utilizes HomogeneousTime-Resolved Fluorescence Resonance Energy Transfer (TR-FRET) technology tomeasure the interaction between CD40 and CD40L. This method enables simple andrapid high-throughput screening of inhibitors and blocking antibodies.
As illustrated, the interactionbetween CD40 and CD40L is detected using an Eu-labeled Anti-Tag1 antibody(TR-FRET donor) and an Acceptor-labeled Anti-Tag2 antibody (TR-FRET acceptor).The binding of CD40 to CD40L brings the donor and acceptor antibodies intoproximity. Excitation of the donor antibody then triggers FRET to the acceptorantibody, resulting in a specific emission signal at 665 nm. The intensity ofthis specific signal is proportional to the degree of CD40/CD40L interaction.This homogeneous assay is simple to perform and requires no washing steps.
Components
Table 1. Kit Components and Storage Conditions
Component |
Concentration |
100 TESTS |
500 TESTS |
2500 TESTS |
10000 TESTS |
Storage Temp. |
|---|---|---|---|---|---|---|
Tag1-CD40 protein |
100× |
4μL |
20μL |
100μL |
400μL |
-80°C |
Tag2-CD40L protein |
100× |
4μL |
20μL |
100μL |
400μL |
-80°C |
Selicrelumab (12.5μM) |
100× |
3μL |
10μL |
50μL |
200μL |
-80°C |
Anti-Tag1 Eu dye antibody |
50× |
10μL |
50μL |
250μL |
1mL |
-80°C |
Anti-Tag2 Acceptor antibody |
12.5× |
40μL |
200μL |
1mL |
4mL |
-80°C |
Detection buffer |
10× |
400μL |
2mL |
10mL |
40mL |
-80°C |
Shipping Notes
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Exchange/Return Notes
- We offer a 30-day return/exchange service after receiving.
- Final sale items are not eligible for returns or exchanges.
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