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Description
Human FBN1 ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Cell Lysis Buffer: Gently wash adherent cells with ice-cold PBS, then trypsinize and collect cells by centrifugation at 1000×g for 5 minutes. Suspension cells can be collected directly by centrifugation. Wash collected cells three times with ice-cold PBS and resuspend in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freeze-thaw cycles or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and remove the supernatant for analysis. 5. Cell Culture Supernatant: Centrifuge at 1000×g for 20 minutes. Remove the supernatant for analysis or store at -20°C or -80°C, avoiding repeated freeze-thaw cycles. 6. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a fibrillin 1 (FBN1) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the fibrillin 1 (FBN1) content in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Fibrillin 1 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Fibrillin-1 (FBN1) is a protein encoded by the FBN1 gene located on chromosome 15. It is a 230 kb gene with 65 coding exons that encodes a 2,871-amino acid proprotein named profibrillin. This proprotein is cleaved near its C-terminus by the furanyl invertase protein to yield fibrillin-1, a member of the fibrin family, and asprosin, a 140-amino acid protein hormone. It is a large extracellular matrix glycoprotein that serves as a structural component of 10-12 nanometer calcium-binding microfibrils. These microfibrils provide force-bearing structural support in elastic and inelastic connective tissues throughout the body. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenate, cell lysate, cell culture supernatant and other biological fluids |
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4.5 ★★★★★
Based on 28 reviews
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Product Reviews
★★★★★ 4
Very soft. Light Blue is more of a sky blue
Color: Light Blue, Size: Throw XL (50" X 70")
Very soft, pretty thin, and a little stretchy. Overall, it looks nice. I would say it's a little thin for the colder weather, but great for the warmer months. I got the light blue, and it is a little more sky blue than true baby blue. I wish it were a true blue, but it's close enough that I'll keep it rather than go through the return process. I washed it, and little fuzzies came free, but nothing too alarming for a first wash. Hopefully, it doesn't do that every time. With how thin it is, there'll be nothing left eventually.
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Reviewed in the United States on April 28, 2026
★★★★★ 5
soft and comfortable
Color: 01 Sage Green, Size: Throw (50" X 60")
Super soft and warm.. Perfect for a quick nap or as a blanket on your bed. Warm but not heavy, so perfect weight and comfort. I ordered the green and color is true to picture. So happy I have it for these rainy days to grab a quick nap,
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Reviewed in the United States on May 25, 2026
★★★★★ 5
Super soft and strong , really nice blanket
Color: Navy, Size: Queen (90" X 90"), Color: Navy, Size: Queen (90" X 90")
This blanket is so soft. This blanket has stretch to it which is nice for tucking under mattress. Easy to lay out and fits perfectly on a queen bed, perfect amount of hangover. Great quality blanket.
I did wash before I put on the bed and it did great. Shed in dryer but that’s easy clean up in vent.
No shedding on bed after washed. I laid on bed before washed and it only shed a small amount, not much.
I absolutely love this material, strong yet super soft.
Definitely a great blanket to buy! You’ll love it.
This blanket is so soft. This blanket has stretch to it, which is nice for tucking under the mattress. It is easy to lay out and fits perfectly on a queen bed, with the perfect amount of hang-up. It is a great quality blanket.
I did wash it before I put it on the bed, and it did great. It shed in the dryer, but that’s easy to clean up in the vent.
No shedding on the bed after it was washed. I laid on the bed before it was washed, and it only shed a small amount, not much.
I absolutely love this material; it is made of strong materials yet super soft.
Definitely a great blanket to buy! You’ll love it. I ordered a white one for our other bedroom.
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Reviewed in the United States on April 27, 2026
★★★★★ 5
Super soft and cozy blanket
Color: 01 Sage Green, Size: King (108" X 90")
Perfect. Soft and warm.
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Reviewed in the United States on May 18, 2026
★★★★★ 5
soft, cozy, lightweight. Drapes nicely or folds flat & even. Warm as liner to another blanket
Color: Orange, Size: Twin(60"x80"), Color: Orange, Size: Twin(60"x80")
Summer weight, soft feel, cozy. If using in cooler temps, then consider using it as a liner, then toss another blanket on top. Nice cozy way to trap in heat.
I love orange and was looking for a functional throw blanket. I didnt want the typical lap blanket size (50"x60"). I am tall and wanted something I can tuck my feet under and pull up to my chest when on the couch. But I also wanted it to be decorative when not in use. Not wanting a thick blanket for this purpose, I looked for a thin one of this style. It folds small and flat, OR drapes nicely when tossed. Even at twin size (60"x 80"), it still works as a "throw" blanket. It measures a little wider and aittle shorter so not perfect twin length.
It is thin, but not see through. Very soft. Haven't washed it yet, so hopefully it holds up.
ALSO, we have cats and dogs, so I am always looking at something to see if it can also be used for them. Depending on size, these blankets would make good furniture covers. Nicer than sheets, cheaper than pet products, thin enough to toss in washer, and a material that will stay in place on fabric furniture. Or just a blankey for pets that like to get cozy.
WHICH, at the time of my writing this, my recent rescue dog from the south is experiencing the cool fall temps of fall. She is snuggled up tight against me. I have this blanket as the base on me and the couch, and then a thicker fleece blanket on top of me and her. I just took a pic and will include it.
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Reviewed in the United States on October 11, 2023
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