Mouse IL-3 ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti mouse IL-3 capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-3 in the sample binds to the solid-phase antibody. After thorough washing to remove unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After thorough washing again, TMB chromogenic substrate was added to avoid light for color development. The depth of color response has a positive correlation with the concentration of IL-3 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value under the condition of 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, biotin labeled IL-3 detection antibody, standard dilution, detection buffer, sa-hrp, TMB chromogenic substrate, washing solution, termination solution, plate sealing membrane and instructions.

Sensitivity: 7.77 pg/ml

Detection Range: 78.13-5000 pg/ml

Recovery Range: 90-116%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 3 (IL-3) is encoded by the IL3 gene in mice. It is a biological signal (cytokine)It can be used as a part of the immune system to improve the innate immune response to disease. It is secreted by basophils and activated T cells and can promote the growth and differentiation of bone marrow-derived T cells in the immune response. IL-3 acts by binding to IL-3 receptor, which can stimulate multipotent hematopoietic stem cells to differentiate into myeloid progenitor cells, or together with IL-7, stimulate multipotent hematopoietic stem cells to differentiate into lymphoid progenitor cells. In addition, IL-3 together with other cytokines (such as erythropoietin (EPO), granulocyte macrophage colony-stimulating factor (GM-CSF), and IL-6) stimulate the proliferation of all myeloid cells (granulocytes, monocytes, and dendritic cells).