Mouse IL-9 ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti- mouse IL-9 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-9 in the sample combines with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-9 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, biotin labeled IL-9 detection antibody, standard dilution, detection buffer, sa-hrp, TMB chromogenic substrate, washing solution, termination solution, plate sealing membrane and instructions.

Sensitivity: 3.59 pg/ml

Detection Range: 125-8000 pg/ml

Recovery Range: 95-117%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 9 (IL-9)It is a glycosylated protein with a molecular weight of 14kda and contains 10 cysteine residues involved in disulfide bonding. It is a cytokine produced by T cells, especially cd4+ helper cells, and can regulate a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It acts by binding to the IL-9 receptor and can activate different signal transduction and activation (STAT) proteins, thus participating in a variety of biological processes. The gene encoding this cytokine has been identified as a candidate gene for asthma. Genetic studies of asthma in mouse models have shown that this cytokine is a decisive factor in the pathogenesis of bronchial hyperresponsiveness. The presence of IL-9-mediated autocrine loops implies the presence of some malignancies such as Hodgkin's disease. IL-9 is expressed by Lee Stokes cells, Hodgkin lymphoma cells and some large aplastic lymphoma cells, but not by non Hodgkin lymphoma and peripheral T-cell lymphoma.