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Description
Mouse FcR Blocking ReagentProduct Specification Host Rat Synonyms anti mouse CD16 CD32 Location Membrane Clone Number 2. 4G2 Antibody Type Recombinant mAb Isotype IgG2b, Application ICC, FCM, Fc receptor blocking Reactivity Ms Concentration 0. 5mg ml Conjugation Unconjugated Physical Appearance Liquid Storage Buffer PBS pH7. 4, 0. 03% Proclin 300 Stability & Storage 12 months from date of receipt reconstitution, 2 to 8 C as supplied Dilution application dilution species ICC 1:
Product Specification
| Host | Rat |
| Synonyms | anti-mouse CD16/CD32 |
| Location | Membrane |
| Clone Number | 2.4G2 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG2b,κ |
| Application | ICC, FCM, Fc receptor blocking |
| Reactivity | Ms |
| Concentration | 0.5mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS pH7.4, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, 2 to 8 °C as supplied |
Dilution
| application | dilution | species |
| ICC | 1:500 | |
| FCM | 1:2000 | |
| Fc receptor blocking | 1.0ug (2μl/test) per 10^6 cells in 100ul volume |
Background
This antibody recognizes the same epitope as clone 2.4G2. All of the Fcγ receptors (FcγR) belong to the immunoglobulin superfamily and are the most important Fc receptors for inducing phagocytosis of opsonized (marked) microbes. This family includes several members, FcγRI (CD64), FcγRIIA (CD32), FcγRIIB (CD32), FcγRIIIA (CD16a), FcγRIIIB (CD16b), which differ in their antibody affinities due to their different molecular structure. CD16/CD32 are expressed on natural killer cells, monocytes, macrophages, dendritic cells (at low levels), Kupffer cells, granulocytes, mast cells, B lymphocytes, immature thymocytes, and some activated mature T lymphocytes.
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Exchange/Return Notes
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