Mouse IL-4 High Sensitivity ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA technology. Specific anti- mouse IL-4 capture antibody was pre coated on a high affinity microplate. Add the standard and the sample to be tested into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The IL-4 in the sample binds to the solid-phase antibody. After thorough washing to remove free and unbound components, biotinylated detection antibody was added for incubation. After washing again, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing, signal enhancer was added for incubation. After washing to remove unbound material, sa-hrp was added again. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of IL-4 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: Pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, biotin labeled IL-4 detection antibody, standard dilution, detection buffer, signal enhancer, signal enhancer dilution, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 0.08 pg/ml

Detection Range: 0.78-50 pg/ml

Recovery Range: 75-93%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Interleukin 4 (IL-4)Is a monomeric cytokine of about 18-20kda. Mouse IL-4 precursor consists of 140 amino acids, 20 amino acids of signal peptide, and 120 amino acids of mature peptide chain. The homology of mature mouse IL-4 with human and rat IL-4 was 43% and 63%, respectively.

IL-4 can induce naive helper T cells (Th0 cells) to differentiate into Th2 cells. After being activated by IL-4, Th2 cells then secrete IL-4 in a positive feedback manner. The cell type that secretes IL-4 to induce Th0 cell differentiation has not yet been identified, but recent studies have shown that basophils may be this effector cell. IL-4 is closely related to IL-13 and has similar functions.

IL-4 can also promote rhabdomyosarcoma to undergo mitosis, dedifferentiation and metastasis. It can be observed that IL-4 and other Th2 cytokines are involved in airway inflammation in allergic asthma patients.