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Description
Organotal rat normal small intestinal organoid culture KitProduct Specification Usage 1. Primary (1) the normal small intestine tissues of rats after sampling must be cleaned at 2 8 , several Petri dishes should be prepared, and primary culture buffer B pre cooled at 4 should be added for standby (double antibody and gentamicin should be added) (2) put the tissue into the Petri dish, wash it three times with primary culture buffer B, remove impurities, remove the intestinal mucosa of normal small intestine
Product Specification
| Usage |
1. Primary (1) the normal small intestine tissues of rats after sampling must be cleaned at 2-8 ℃, several Petri dishes should be prepared, and primary culture buffer B pre cooled at 4 ℃ should be added for standby (double antibody and gentamicin should be added) (2) put the tissue into the Petri dish, wash it three times with primary culture buffer B, remove impurities, remove the intestinal mucosa of normal small intestine tissue of rats with ophthalmic scissors or scalpel, and cut it into a tissue block with a volume of about 10-30mm3 (3) the tissue was digested with rat normal small intestine primary tissue digestive solution C, and then shaken at 4 ℃ for 20-25min or left to digest for 40-50min (observe the digestion at any time during the digestion process) (4) take a small amount of liquid and observe under the microscope. More crypts are observed under the microscope. Add three times the volume of primary culture buffer B to stop digestion (5) filter with a 100um pore size screen, collect the filtrate, remove the supernatant after centrifugation at 300g for 5min, add primary culture buffer B and resuspend for centrifugation (6) Matrigel calculation: after step 5, observe the collected tissue volume, add 25 times the tissue volume of Matrigel (abs9495) to resuspend the plate (7) take a 24 well cell culture plate as an example, dispense 25ul of tissue Matrigel mixture per well for plating (operate at 4 ℃) (8) put the laid culture plate into a 37 ℃ incubator for 10-15min to form a glue, and add rat normal small intestinal organoid medium a (restore room temperature) for culture 2. Organoid subculture (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) a: the number of organoids is insufficient or the volume is small: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4 b: When the number of organoids is large or the volume is large: centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid passage digest D for digestion for 2-3min, add organoid passage culture buffer g to stop digestion, centrifuge for 5min to discard the mixture, add an appropriate amount of organoid passage culture buffer g to resuspend and transfer into a 1.5ml centrifuge tube, centrifuge for 5min at 300g to discard the liquid for step 4 (4) after organoids were collected, add Matrigel to resuspend, lay 25ul Matrigel per well in a 24 well cell culture plate, place in the incubator for 10-15min, and add 500ul of rat normal small intestinal organoid culture medium a 3. Organoid cryopreservation (1) pipette the culture medium, add 1-2ml of 4 ℃ organoid subculture buffer g to each well and place it for 2min (2) pipette the Matrigel gently, collect it in a 15ml centrifuge tube, and let it stand at 4 ℃ for 10min. (each 6-8 well is a group) (3) centrifuge for 5min to discard the supernatant, add an appropriate amount of organoid subculture buffer g to resuspend, and centrifuge for 5min at 300g to discard the liquid (4) add an appropriate amount of organoid cryopreservation solution F, gently blow and resuspend. Take a 24 well cell culture plate as an example: the density is 2 wells and 1 tube is frozen, with a volume of 1.4ml per tube (5) mark the information, and after cooling down the program, transfer it to liquid nitrogen for long-term storage 4. Organoid resuscitation (1) take 10ml of organoid subculture buffer g and put it into a 15ml centrifuge tube (2) take out the frozen organoid cells from the liquid nitrogen tank and quickly put them into a 37 ℃ water bath pot for melting (3) during the water bath thawing process, it is necessary to gently shake the freezing tube to ensure that the frozen solution is completely thawed in 1-2min (4) quickly transfer the dissolved organoid cells to a 15ml centrifuge tube, gently blow 6-8 times with a pipette, centrifuge at 300g for 5min, and then remove the supernatant and collect the organoid cell pellet. Add an appropriate amount of organoid subculture buffer g to resuspend and transfer into a 1.5ml centrifuge tube. Centrifuge for 5min at 300g (5) resuspend Matrigel, lay 25ul Matrigel per well in a 24 well cell culture plate, place in an incubator for 10-15min to form a gel, and add 500ul of rat normal small intestinal organoid culture medium A. |
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| Synonym | Rat Small Intestine Organoid Culture Kit | ||||||||||||||||
| Description | Composition:
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| Storage Temp. | 4 ℃, 3months; -20 ℃, 1 year, see the label for details |
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Product Reviews
★★★★★ 5
Amazing!
Size: 2.5 Fl Oz (Pack of 1)
This sunscreen is amazing! I love that it goes on blue and then turns clear. It’s helping my kids learn to put it on themselves. It also stays on good and we haven’t had any sunburns even on the beach.
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Reviewed in the United States on May 19, 2026
★★★★★ 5
Great product
Size: 2.5 Fl Oz (Pack of 1)
We really love the roll-on application of the sunscreen. It's great quality and ease for use is perfect for both parents and kids to use. It does require some extra rubbing in after rolled on, but it's perfect in order to see any areas that may or may not have been missed. I recommend and will purchase again.
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Reviewed in the United States on November 5, 2025
★★★★★ 5
Great sunscreen!
Size: 2.5 Fl Oz (Pack of 1)
My kids used to hate putting sunscreen on, but with this one they do it without complaining. The light green color makes it easy to see, and the roll-on design is super simple for kids to use. They actually enjoy it. It feels like they’re painting their face and body, which makes it fun. The only downside is that it leaks a little.
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Reviewed in the United States on September 1, 2025
★★★★★ 5
Good protection, easy to tell where it was applied
Size: 2.5 Fl Oz (Pack of 1)
Putting sunscreen on kids can be a struggle. This product does many things well: easy to put on, no stinging eyes or coughing like there is with spray, provides good coverage, lasts a long time while sweating, and blocks sun well. But the really cool part is it goes on blue initially (dries clear/slightly white). This is helpful when my little one puts on his own sunscreen because it makes it easier to see the spots he missed (usually many) and helps ensure he fills in the gaps.
Also great for the adults - I used it on vacation when I ran out of mine and it worked very well (great if you have arm hair too - not as sticky as many others)
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Reviewed in the United States on September 1, 2023
★★★★★ 4
Nice sunscreen, not very blue
Size: 2.5 Fl Oz (Pack of 1)
Rolls on/spreads very nicely. Not too sticky and my toddler doesn’t mind it the way she does most other sunscreens. Seems to protect well. It’s fun “blue” color is extremely faint and the product itself is closer to cloud white than sky blue. I would imagine it would barely show up at all on darker skin tones but still not complaining as she is letting me apply it despite the absence of the ‘finger painting’ I had tried to get her excited for.
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Reviewed in the United States on May 27, 2025
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