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Description
mIHC Dye (TSA620)Product Specification References 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008. 9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79 80, 105. Stack, E. C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46 58. 4.
Product Specification
| References | 1. Dabbs David J. Diagnostic immunohistochemistry (M). Beijing: Peking University Medical Press, 2008.9. 2. [US] Ed Harlow, David Lane Technical Guide (M). Beijing: Science Press, 2002: 79-80, 105. Stack, E.C., et al., Multiplexed immunohistochemistry, imaging, and uantitation: are view, with an assessment of Tyramide signal amplification, multispectral imaging and multiplex analysis. Methods, 2014; 70 (1): 46-58. 4. Qian Bangguo Jiao Lei Application of multi-label immunofluorescence staining and Doppler imaging technology in histological research. Chinese Journal of Histochemistry and Cytochemistry, 2017 (4): 373-382. |
| Theory | Multiplex fluorescence immunohistochemical staining is based on the principle of specific binding of antigen and antibody. The secondary antibody labeled with horseradish peroxidase (HRP) is selected to activate the fluorescent dye in the kit and covalently bind the signal to the antigen. In situ multi-target staining of tissues or cells is achieved by multiple rounds of staining cycles with the aid of different fluorescent dye labels. |
| Description | There are complex cells in tissue microenvironment, and the phenotype, state, abundance and distribution of these cells have important biological significance and clinical value. It can be rendered in situ in tissue by means of antibody staining. Immunohistochemical staining is a common technique to study tissue morphology and protein expression in situ. Routine IHC detection can only show a single indicator, and it is difficult to present the cell composition, state and relationship in the complex tissue microenvironment. This information is crucial to the diagnosis and treatment of diseases! Principle of tyrosine signal amplification technology: Similar to the DAB color development method of conventional immunohistochemistry, TSA technology also uses HRP-labeled secondary antibody. HRP catalyzes the fluorescein substrate added to the system to produce an activated fluorescein substrate, which can be covalently bound to the antigen. Tyrosine is covalently bound to stably covalently bound to fluorescein on the sample. After that, the non-covalently bound antibody is washed away by thermal repair method, replaced with a primary antibody for the second round of incubation, and replaced with another fluorescein substrate, so that multiple labeling can be achieved by reciprocating. |
| Composition | TSA monochromatic fluorescent dye 620, signal amplification reaction solution |
| General Notes | 1. Formalin-fixed wax blocks or glass slides, large tissues or TMA, and sealing wax cannot be obviously damaged. 2. For glass slide samples, the tissue needs to be close to the glass slide to avoid wrinkles, and the glass slide must not be damaged, scratched or stained. 3. The tissue should contain at least more than 1000 cells. 4. The wax block needs to be embedded in solid tumor tissue, and necrotic tumor tissue, fine needle punctures, and cell slice samples will affect the staining effect. 5. The tissue should be fixed with 10% neutral formalin, and the normal fixation time is 18-24h. 6. The thickness of the slice is about 4um, and anti-detachment slides are used. It is recommended that the glass slides be prepared within one week after fixation. 7. Do not add any adhesive in the water bath slide fishing process. The sample should be placed on the front and center of the slide. Place the slide vertically on absorbent paper to remove water, tap gently to remove water droplets, and do not wipe the slide with paper. 8. Place the glass slide on a hot plate at 45 °C for 30min (the natural air-drying time of the glass slide is not less than 1h). |
| Storage Temp. | Fluorescent dyes should be stored in the dark from light at 2-8 ℃, and the validity period is 12 months from receipt of the goods. |
| Applications | It is mainly used for scientific research on immunohistochemical staining of tissues, paraffin sections and TMA chips, and cannot be used for in vitro clinical diagnosis or human experiments. |
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4.9 ★★★★★
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Product Reviews
★★★★★ 5
The Perfect Pad
I love these pads. Soft and efficient. After my last brand changed the quality of the pad and were rough and scratchy I was delighted find these!
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Reviewed in the United States on July 17, 2024
★★★★★ 5
These are great
I love these make up remover pads they are so great I had to buy some more.
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Reviewed in the United States on November 18, 2024
★★★★★ 5
Awesome for hyper dogs
Color: Blue
AWESOME - I have a dog that just won't stop trying to get me to play with him ALL day! I turn this thing on and he goes nuts and tires himself out...without any help from me. The cover got torn off and torn up in minutes but he doesn't care. He just wants this ball. My other dogs, who are older, aren't really interested. But if you have a hyper dog this is the thing for you and them!
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Reviewed in the United States on April 28, 2026
★★★★★ 5
Great entertainment, charges quickly!
Color: Orange
Super fun entertainment for dog! Toy ball charges quickly and the charge lasts for at least an hour of playtime for the dog. There are 2 modes. one mode Just lights up, which makes it easy for pet to chase it. The second mode not only lights up, but it also wiggles on the ground Randomly and speeds off in a direction on its own, which really makes it interesting for your dog or cat to follow! Once the ball is not touched for about thirty seconds, it goes dormant and it will stay like that As long as it's charged until somebody touches it, and then it starts up randomly lighting up and moving around the floor on its own And making a vibration noise. It's a lot of fun to watch the dog chase and play with, and it certainly keeps them entertained for a while! The laughs and fun entertainment are definitely worth it. The only downside is that my dog chewed off the soft fabric covering in about twenty minutes.And she's not a real big chewer. It looks better with the covering on it, but even without it, the ball works fine and she plays with it every day.So that is not a showstopper. But good to supervise your dog for safety.In case they are a chewer, and they decide to destroy the cover. But the ball works fine without the cover too!
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Reviewed in the United States on March 12, 2026
★★★★★ 4
Looooves her crazy new ball
Color: Orange, Color: Orange
Our 90lb mastiff/boxer mix Indie is very playful, curious and energetic. She needs toys that engage her and keep her busy. This ball does the job!
We’ve had it for less than a week. Here’s what I love and what could be better:
PROS:
• She dog loves it. She whines for it. She gets absurdly excited when I get it out. She’s a bit of an anxious dog, but had zero problems with it inexplicably coming to life.
• It lasts a while on a single charge, so it keeps her busy for a good long time. The longest we’ve used it at a stretch is an hour.
• It’s pretty durable. She’s a super chewer (destroyed every “non-destructible” toy we’ve purchased for her), but the actual ball is hard plastic. She gnaws on it and it gets scratches, for sure. But she’s not going to chew it to pieces.
• On carpet, it’s reasonably quiet.
• It comes with a replaceable silicone button.
• It’s easy to charge…just pull off the silicone button and the charging port is inside.
• The silicone button is flush with the ball’s hard plastic surface, so there’s no way for her to pull the silicone button out and swallow it or get to the charging port.
CONS:
• Cover is worthless if your dog is a chewer. It goes on like an envelope with a Velcro closure. We took it off immediately.
• Without the cover, it’s very noisy on hardwood floors. To us, it’s worth it. (Though, I work from home, so I might feel differently after the holiday vacation.)
• She’s an XL dog, so she can fit the whole ball in her mouth easily and accidentally chomp down on the button and turn the ball off. Not a big deal, just something to know if you’ve got a Lab or larger dog.
• With extended playtime, the two halves can come unscrewed, especially with a large dog who uses their paws/jaws on it a lot. Thankfully, it makes a loud buzzing noise when this happens, so we know we need to take it from her and screw it back together. Is this a potential danger? With the rechargeable battery inside, I’d say yes. But we also don’t let her play with this toy without supervision. So keep that in mind.
Bottom line:
It has its drawbacks, but it keeps her entertained and seems like it should last her at least 6 months with supervised play. Which is honestly REALLY good when we’re talking about Indie, Destroyer of Toys. I’d buy it again.
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Reviewed in the United States on December 31, 2024