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Description
Histone H4 Recombinant Rabbit mAb (S-499-253)Product Specification Host Rabbit Antigen Histone H4 Synonyms Histone H4; H4 A; H4FA; HIST1H4A; H4C2; H4 I; H4FI; HIST1H4B; H4C3; H4 G; H4FG; HIST1H4C; H4C4; H4 B; H4FB; HIST1H4D; H4C5; H4 J; H4FJ; HIST1H4E; H4C1 Immunogen Synthetic Peptide Location Nucleus Accession P62805 Clone Number S 499 253 Antibody Type Recombinant mAb Isotype IgG Application WB, IHC P, ICC Reactivity Hu, Ms, Rb, Rt, Zf, Dg, Ck Positive Sample HeLa, Jurkat, NIH 3T3, mouse
Product Specification
| Host | Rabbit |
| Antigen | Histone H4 |
| Synonyms | Histone H4; H4/A; H4FA; HIST1H4A; H4C2; H4/I; H4FI; HIST1H4B; H4C3; H4/G; H4FG; HIST1H4C; H4C4; H4/B; H4FB; HIST1H4D; H4C5; H4/J; H4FJ; HIST1H4E; H4C1 |
| Immunogen | Synthetic Peptide |
| Location | Nucleus |
| Accession | P62805 |
| Clone Number | S-499-253 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG |
| Application | WB, IHC-P, ICC |
| Reactivity | Hu, Ms, Rb, Rt, Zf, Dg, Ck |
| Positive Sample | HeLa, Jurkat, NIH/3T3, mouse brain, C6, rat brain, zebrafish, dog liver, rabbit liver, pig heart, chicken liver |
| Predicted Reactivity | Fu, Dr, Pl, Ys |
| Purification | Protein A |
| Concentration | 0.5 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000 | Hu, Ms, Rt, Zf, Dg, Rb, Ck, Pg |
| IHC-P | 1:2000 | Hu, Ms, Rt |
| ICC | 1:500 | Hu, Ms |
Background
Histone H4 is a core component of the nucleosome, the basic structural unit of chromatin in eukaryotic cells, consisting of 102 amino acids that are highly conserved across species and subject to extensive post-translational modifications—such as acetylation, methylation, phosphorylation, and ubiquitination—that collectively regulate chromatin compaction, DNA replication, repair, and transcriptional activity by altering the electrostatic interaction between DNA and the histone octamer or by recruiting specific chromatin-binding proteins.
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