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Description
TdT Recombinant Mouse mAb (SDT-4203)Product Specification Host Mouse Antigen TdT Synonyms DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase (Terminal transferase); TDT; DNTT Location Nucleus Accession P04053 Clone Number SDT 4203 Antibody Type Recombinant mAb Isotype IgG2a Application WB, IHC P Reactivity Hu Positive Sample Jurkat, Molt 4, mouse thymus, rat thymus Purification Protein A Concentration 2 mg ml Conjugation Unconjugated Physical
Product Specification
| Host | Mouse |
| Antigen | TdT |
| Synonyms | DNA nucleotidylexotransferase; Terminal addition enzyme; Terminal deoxynucleotidyltransferase (Terminal transferase); TDT; DNTT |
| Location | Nucleus |
| Accession | P04053 |
| Clone Number | SDT-4203 |
| Antibody Type | Recombinant mAb |
| Isotype | IgG2a |
| Application | WB, IHC-P |
| Reactivity | Hu |
| Positive Sample | Jurkat, Molt-4, mouse thymus, rat thymus |
| Purification | Protein A |
| Concentration | 2 mg/ml |
| Conjugation | Unconjugated |
| Physical Appearance | Liquid |
| Storage Buffer | PBS, 40% Glycerol, 0.05% BSA, 0.03% Proclin 300 |
| Stability & Storage | 12 months from date of receipt / reconstitution, -20 °C as supplied |
Dilution
| application | dilution | species |
| WB | 1:1000-1:5000 | Hu, Ms, Rt |
| IHC-P | 1:2000 | Hu |
Background
Terminal deoxynucleotidyl transferase (TdT), also called DNA nucleotidylexotransferase or terminal transferase, is a template-independent DNA polymerase encoded by the DNTT gene on human chromosome 10q23-q24 that is selectively expressed in immature lymphoid precursors (pre-B and pre-T cells) and certain leukemias/lymphomas; it catalyzes the non-templated addition of deoxynucleotides to the 3'-OH ends of DNA, generating N-region diversity at V(D)J junctions during immunoglobulin and T-cell receptor gene rearrangement, thereby expanding antigen-receptor repertoires critical for adaptive immunity, and can utilize a broad range of divalent cations (Mg²⁺, Mn²⁺, Zn²⁺, Co²⁺) as cofactors .
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