Human sCD163 ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA technology. The specific anti human CD163 capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The CD163 present in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of CD163 in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: double antibody sandwich method

Form: pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, CD163 detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 4.39pg/ml

Detection Range: 62.5-4000 pg/ml

Recovery Range: 88-110%

Storage Method: 2-8 ℃

Standard Curve:

Background:

CD163 is a protein encoded by the CD163 gene in humans. It belongs to the cysteine rich B-type scavenger receptor family, and contains an extracellular domain consisting of 1048 amino acid residues, a single transmembrane segment, and a cytoplasmic tail region with several splicing variations. It is a hemoglobin binding beadScavenger receptors for protein complexes that label monocyte / macrophage lineages. The soluble form of this receptor is present in plasma and is usually expressed as scd163. Scd163 is produced by the ectodomain shedding of membrane-bound receptors and is upregulated in a wide range of inflammatory diseases including liver cirrhosis, type 2 diabetes, macrophage activation syndrome, Gaucher disease, sepsis, HIV infection, rheumatoid arthritis and Hodgkin lymphoma.