Human BDNF ELISA Kit

Detection Principle:

This kit uses double antibody sandwich ELISA technology. The specific anti human BDNF capture antibody was pre coated on a high affinity microplate. Add the standard, the sample to be tested and the biotin labeled detection antibody into the wells of the enzyme plate in turn, shake well and mix well, and then place it at room temperature for 2 hours of incubation process. The BDNF present in the sample is combined with the solid-phase antibody and the detection antibody. After washing sufficiently to remove free and unbound components, streptavidin HRP (sa-hrp) labeled with horseradish peroxidase was added. After washing again, TMB chromogenic substrate was added and incubated at room temperature in the dark to develop color. The depth of color response is positively correlated with the concentration of BDNF in the sample. Add stop solution to stop the reaction, and use a microplate reader to measure the absorbance value at 450 nm detection wavelength (correction wavelength 570-630 nm).

Detection Type: Double antibody sandwich method

Form: pre coated 96 well plate

Test Sample Type: cell supernatant, serum, plasma

Loading Amount: 100 μ L

Kit Components: A copy of pre coated 96 well plate, standard, BDNF detection antibody, standard dilution, detection buffer, TMB chromogenic substrate, washing solution, termination solution, sa-hrp, plate sealing membrane and instructions.

Sensitivity: 1.41pg/ml

Detection Range: 7.81-500 pg/ml

Recovery Range: 85-115%

Storage Method: 2-8 ℃

Standard Curve:

Background:

Brain derived neurotrophic factor (BDNF)It is a member of the neurotrophin family of growth factors and is related to nerve growth factors. Neurotrophins exist in brain and periphery. BDNF is processed and synthesized in the endoplasmic reticulum and secreted by dense core vesicles. It is active in the hippocampus, cortex, and basal forebrain regions of the brain, as well as in the retina, motor neurons, kidneys, saliva, and prostate. BDNF acts on some neurons of the central nervous system and peripheral nervous system, helping the survival of existing neurons and promoting the growth and differentiation of newborn neurons and synapses. The changes of BDNF may play a role in the pathogenesis of schizophrenia, and are related to depression, eczema, epilepsy, Alzheimer's disease, obesity, aging, congenital central hypoventilation syndrome and cognitive dysfunction after chemotherapy,