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Description
Mouse FOXP3 ELISA KitProduct Specification Usage Required experimental equipment: 1. Microplate reader (450nm) 2. High precision pipettes and pipette tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre chilled PBS (0. 01M, pH 7. 4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and
Product Specification
| Usage | Required experimental equipment: 1. Microplate reader (450nm) 2. High-precision pipettes and pipette tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37°C incubator 4. Distilled or deionized water Sample preparation and requirements: Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh and mince the tissue. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1g of tissue sample to 9mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000×g for 5-10 minutes, and collect the supernatant for analysis. Cell Lysis Buffer: Adherent cells should be gently washed with pre-chilled PBS, then trypsinized and harvested by centrifugation at 1000×g for 5 minutes. Suspension cells can be harvested directly by centrifugation. Collected cells should be washed three times with pre-chilled PBS and resuspended in 150-200 μL of PBS per 1×10^6 cells (it is recommended to add protease inhibitors to the PBS; if the cell count is very low, reduce the PBS volume appropriately). Disrupt the cells by repeated freezing and thawing or sonication. Centrifuge the extract at 1500×g for 10 minutes at 2-8°C, and collect the supernatant for analysis. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 20 ng/mL). Then dilute to the following concentrations: 20 ng/mL, 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, and 0 ng/mL. Serial dilution method: Take 7 EP tubes and add 500 μL of universal diluent to each tube. Pipette 500 μL of the 20 ng/mL standard working solution into the first EP tube and mix thoroughly to make a 10 ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves directly as a blank well; there is no need to aspirate the liquid from the penultimate tube. See the figure below for details. 3. Preparation of Biotinylated Antibody Working Solution: 15 minutes before use, centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration using universal diluent (e.g., 10µL concentrate + 990µL universal diluent). Prepare immediately before use. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit utilizes a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a capture antibody against Forkhead Box Protein P3 (FOXP3). After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by HRP peroxidase and to yellow by acid. The intensity of the color is positively correlated with the amount of Forkhead Box Protein P3 (FOXP3) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Mouse | |||||||||||||||||||||||||||||||||
| Synonym | Mouse Forkhead Box Protein P3 ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Forkhead P3 (FOXP3), also known as scurfin, is a protein involved in immune system responses. As a member of the FOX protein family, FOXP3 plays a master regulatory role in the development and function of regulatory T cells (RTCs). TRCs typically downregulate immune responses. FOX proteins belong to the forkhead/winged-helix family of transcriptional regulators and are hypothesized to exert control during transcription through similar DNA-binding interactions. In a TRC model system, the FOXP3 transcription factor occupies the promoters of genes involved in TRC function and may repress transcription of key genes upon T cell receptor stimulation. The FOXP3 gene contains 11 coding exons. Genomic sequence analysis has mapped the FOXP3 gene to the p arm of the X chromosome. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.31-20 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Tissue homogenates, cell lysates, and other biological fluids |
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4.2 ★★★★★
Based on 26 reviews
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Product Reviews
★★★★★ 5
Simple, hefty configurable small tray
Color: Black, Color: Black
"Hefty" is not an expected description for a drawer organizer. But it is in this case because of the solid metal plate it's made from. You could brain a zombie with this tray and not dent it (the tray... the zombie's the one that would be dented). Guess the material choice does make sense since the two wooden dividers uses magnets to hold positions. A strong bump would be needed to shift the placed dividers.
You get some basic configuration choices from the dividers. Including additional (different length) dividers would allow further sectioning options would have been nice.
It also looks good too.
Like the design and utility. Would also go for a bigger version. Perhaps that one, like this, would have secondary uses... perhaps as armor plating?
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Reviewed in the United States on March 19, 2026
★★★★★ 5
Elegant and easy to customize to organize your desk
Color: Black, Color: Black
This desk organizer was higher quality and more elegant looking than I thought it would be, so I decided to use it to hold some things on my dresser. It’s too pretty to hide away in my drawer.
The compartments snap into place with magents so you can adjust based on how you want to organize your items. The tray itself is heavy, durable, and well-made. The compartments and edges seem to be authentic wood, and the base surface of the tray is a rubber-like surface that keeps things from slipping while still looking very nice.
I like this tray enough to put it on display as a part of my home decor. It’s not only useful for organizing some odds and ends, but is also very beautiful and well made.
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Reviewed in the United States on April 12, 2026
★★★★★ 4
Stylish Aesthetic with Functional Limitations
Color: Black
This desk organizer offers a sophisticated look that complements modern furniture and decor quite well. The combination of aluminum and walnut creates a professional appearance that effectively tidies up a workspace or nightstand. I particularly appreciate the soft-touch base, which provides a safe surface for delicate items like phone screens or watches, ensuring they remain free of scratches. Additionally, the materials are very easy to wipe down and maintain.
However, the build quality does not entirely justify the premium price point. The aluminum components feel more like thin, shaped sheet metal rather than a solid, high-end material. While the walnut accents are a nice touch, they lack the weight and substance I expected for a valet tray in this category.
The primary functional drawback lies in the magnetic adjustment system. The magnets are relatively weak, meaning that accidentally bumping a divider while reaching for an item can easily shift the layout. This requires frequent readjustments, which can be frustrating during a busy workday. Furthermore, the magnetic rails can interfere with other metallic or magnetic objects you might try to store. If you are looking for a purely aesthetic upgrade to organize lightweight items, this tray serves that purpose, but it falls short in terms of stability and material density.
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Reviewed in the United States on April 7, 2026
★★★★★ 5
Love the customizable dividers
Color: Black, Color: Black
I really like this desk drawer organizer tray. It comes with two removable adjustable dividers that are magnetic. They have a strong magnetic pull and stay in place and do not move or shift at all. I love the overall look of it. It’s very sophisticated looking. It can be suitable for a man or a woman. If it’s into my drawer perfectly and I love the smooth modern design of it.
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Reviewed in the United States on April 3, 2026
★★★★★ 5
A nice customizable organizer.
Color: Black, Color: Black
This is a nice organizer for a drawer or nightstand. Comes in a box that is not overly bulky and is well protected. It's quick and easy to adjust and put together. the seperators are magnetic so you can customize it to fit your needs. There are two cut outs for charging cords and it fits a phone easily along with other accessories. The unit feels solid and well built, it has a good weight to it. I do wish the deviders were a bit easier to get square, but that's more just my idiosyncrasies.
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Reviewed in the United States on March 14, 2026