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Description
Human TAZ ELISA KitProduct Specification Usage Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High precision pipette and gun tips: 0. 5 10uL, 5 50uL, 20 200uL, 200 1000uL 3. 37 constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4 overnight, then centrifuge at 1000g for 20
Product Specification
| Usage |
Experimental equipment required for the experiment: 1. Microplate reader (450nm) 2. High-precision pipette and gun tips: 0.5-10uL, 5-50uL, 20-200uL, 200-1000uL 3. 37℃ constant temperature box 4. Distilled water or deionized water Sample processing and requirements: 1. Serum: Place the whole blood sample collected in the serum separation tube at room temperature for 2 hours or at 4℃ overnight, then centrifuge at 1000×g for 20 minutes, and take the supernatant, or store the supernatant at -20℃ or -80℃, but avoid repeated freezing and thawing. 2. Plasma: Collect the specimen using EDTA or heparin as an anticoagulant. Centrifuge the specimen at 1000 × g for 15 minutes at 2-8°C within 30 minutes of collection. The supernatant can be assayed or stored at -20°C or -80°C, but avoid repeated freezing and thawing. 3. Tissue homogenization: Rinse the tissue with pre-chilled PBS (0.01M, pH 7.4) to remove residual blood (lysed red blood cells in the homogenate will affect the measurement results). Weigh the tissue and mince it. Add the minced tissue to the appropriate volume of PBS (generally a 1:9 weight-to-volume ratio, e.g., 1 g of tissue sample to 9 mL of PBS. The specific volume can be adjusted according to experimental needs and recorded. It is recommended to add protease inhibitors to the PBS) in a glass homogenizer and grind thoroughly on ice. To further lyse tissue cells, the homogenate can be sonicated or repeatedly frozen and thawed. Finally, centrifuge the homogenate at 5000 × g for 5-10 minutes, and the supernatant can be assayed. 4. Other biological fluids: Centrifuge at 1000xg for 20 minutes, remove the supernatant, and test. Pre-test preparation: 1. Remove the test kit from the refrigerator 10 minutes in advance and equilibrate to room temperature. 2. Prepare the standard gradient working solution: Add 1 mL of universal diluent to the lyophilized standard, let it stand for 15 minutes to completely dissolve, then gently mix (concentration is 10 ng/mL). Then dilute to the following concentrations: 10 ng/mL, 5 ng/mL, 2.5 ng/mL, 1.25 ng/mL, 0.625 ng/mL, 0.3125 ng/mL, 0.15625 ng/mL, and 0 ng/mL. Serial dilution method: Take seven EP tubes and add 500uL of universal diluent to each. Pipette 500uL of the 10ng/mL standard working solution into the first EP tube and mix thoroughly to make a 5ng/mL standard working solution. Repeat this procedure for subsequent tubes. The last tube serves as a blank well; there is no need to pipette liquid from the penultimate tube. See the figure below for details. 3. Preparation of biotinylated detection antibody working solution: Centrifuge the concentrated biotinylated antibody at 1000×g for 1 minute 15 minutes before use. Dilute the 100× concentrated biotinylated antibody to a 1× working concentration with universal diluent (e.g., 10uL concentrate + 990uL universal diluent). Prepare and use immediately. 4. Prepare the enzyme conjugate working solution: 15 minutes before use, centrifuge the 100× concentrated enzyme conjugate at 1000×g for 1 minute. Dilute the 100× concentrated HRP enzyme conjugate to a 1× working concentration with universal diluent (e.g., 10 μL of concentrate + 990 μL of universal diluent). Prepare immediately. 5. Prepare the 1× wash solution: Dispense 10 mL of 20× wash solution into 190 mL of distilled water (concentrated wash solution removed from the refrigerator may crystallize; this is normal. Allow to stand at room temperature until the crystals have completely dissolved before preparing). Procedure: 1. Remove the desired strips from the aluminum foil bag after equilibration at room temperature for 10 minutes. Seal the remaining strips in a ziplock bag and return to 4°C. 2. Sample addition: Add 100 μL of sample or standard of varying concentrations to the corresponding wells. Add 100 μL of universal diluent to the blank wells. Cover with a film and incubate at 37°C for 60 minutes. (Recommendation: Dilute the sample to be tested at least 1-fold with universal diluent before adding it to the ELISA plate. This will reduce the impact of matrix effects on the test results. The sample concentration should be multiplied by the corresponding dilution factor when calculating the final sample concentration. It is recommended to run replicates for all test samples and standards.) 3. Add Biotinylated Antibody: Remove the ELISA plate and discard the liquid without washing. Add 100 μL of Biotinylated Antibody Working Solution directly to each well. Cover with a film and incubate at 37°C for 60 minutes. 4. Wash: Discard the liquid and add 300 μL of 1x Wash Solution to each well. Let stand for 1 minute, shake off the wash solution, and pat dry on absorbent paper. Repeat this process three times (a plate washer can also be used). 5. Add Enzyme Conjugate Working Solution: Add 100 μL of Enzyme Conjugate Working Solution to each well. Cover with a film and incubate at 37°C for 30 minutes. 6. Washing: Discard the liquid and wash the plate five times as in step 4. 7. Adding substrate: Add 90 μL of substrate (TMB) to each well, cover with a sealing film, and incubate at 37°C in the dark for 15 minutes. 8. Adding stop solution: Remove the ELISA plate and add 50 μL of stop solution directly to each well. Immediately measure the OD value of each well at a wavelength of 450 nm. Calculating experimental results: 1. Calculate the average OD value of the standard and sample replicates and subtract the OD value of the blank well as a correction factor. Plot the standard curve of the four-parameter logistic function on double-logarithmic graph paper, with concentration as the horizontal axis and OD value as the vertical axis. 2. If the sample OD value is higher than the upper limit of the standard curve, dilute the sample appropriately and retest. Multiply the sample concentration by the corresponding dilution factor. |
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| Theory | This kit uses a double-antibody sandwich enzyme-linked immunosorbent assay (ELISA). Sample, standard, biotin-labeled detection antibody, and HRP conjugate are sequentially added to microwells pre-coated with a tafazzin (TAZ) capture antibody. After incubation and washing, the sample is developed using the substrate TMB. TMB is converted to blue by peroxidase (HRP) catalysis and to yellow by acid. The intensity of the color is positively correlated with the amount of tafazzin (TAZ) in the sample. The absorbance (OD) is measured at 450 nm using a microplate reader to calculate the sample concentration. | |||||||||||||||||||||||||||||||||
| Source | Human | |||||||||||||||||||||||||||||||||
| Synonym | Human Tafazzin ELISA Kit | |||||||||||||||||||||||||||||||||
| Detection Type | Double antibody sandwich method | |||||||||||||||||||||||||||||||||
| Composition |
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| Background | Tafazzin is a protein encoded by the TAFAZZIN gene. Tafazzin is highly expressed in cardiac and skeletal muscle and functions as a phospholipid-glycophospholipid transacylase (a member of the phospholipid:diacylglycerol acyltransferase family). It catalyzes the remodeling of immature cardiolipin into mature components, primarily tetrahydrooleoyl groups. The TAFAZZIN gene produces several distinct tafazzin protein isoforms. Each isoform has a long form and a short form. The short form lacks a hydrophobic leader sequence and may be a cytoplasmic protein rather than a membrane-bound protein. Other alternatively spliced transcripts have been described. Most isoforms are found in all tissues, but some are only found in certain cell types. The TAFAZZIN gene is located at position 28 on the q arm of chromosome X and spans 10,208 base pairs. It produces a 21.3 kDa protein composed of 184 amino acids. Mutations in the TAFAZZIN gene have been associated with mitochondrial defects, Bartter syndrome, dilated cardiomyopathy (DCM), hypertrophic DCM, endocardial fibrosis, left ventricular noncompression (LVNC), breast cancer, papillary thyroid carcinoma, non-small cell lung cancer, glioma, gastric cancer, thyroid tumors, and colorectal cancer. | |||||||||||||||||||||||||||||||||
| General Notes | 1. Strictly adhere to the specified incubation time and temperature to ensure accurate results. All reagents must be at room temperature (20-25°C) before use. Refrigerate reagents immediately after use. 2. Improper plate washing may result in inaccurate results. Ensure that all liquid in the wells is aspirated thoroughly before adding substrate. Do not allow the wells to dry out during incubation. 3. Remove any residual liquid and fingerprints from the bottom of the plate, as this will affect the OD value. 4. The substrate developer solution should be colorless or very light in color. Do not use substrate solution that has turned blue. 5. Avoid cross-contamination of reagents and specimens to prevent erroneous results. 6. Avoid direct exposure to strong light during storage and incubation. 7. Do not expose any reagents to bleaching solvents or the strong fumes emitted by bleaching solvents. Any bleaching agent will destroy the biological activity of the reagents in the kit. 8. Do not use expired products, and do not mix components with different product numbers and batches. 9. Recombinant proteins from sources other than the kit may not be compatible with the antibodies in this kit and will not be recognized. 10. If there is a possibility of disease transmission, all samples should be managed properly and samples and testing devices should be handled according to prescribed procedures. |
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| Storage Temp. | If the unopened kit is stored at 4°C, the shelf life is 6 months. | |||||||||||||||||||||||||||||||||
| Test Range | 0.15-10 ng/mL | |||||||||||||||||||||||||||||||||
| Applications | Serum, plasma, tissue homogenates and other biological fluids |
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4.4 ★★★★★
Based on 8 reviews
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Product Reviews
★★★★★ 4
Perfect EXCEPT for the Noise
Size: 30mm, Color: Pink/Rose Gold-Tone/Cream/30mm, Size: 30mm, Color: Pink/Rose Gold-Tone/Cream/30mm
My ideals for a watch are: full numerals, easy to read (good contrast and numbers, lines for the minutes and hours), light up function, case under 33mm, swappable bands, and preferably cute, in either silver color or rose gold color.
This one checks all the boxes... And is the only one I've found which does. You can see on the picture that I replaced the band.
Now here's the problem... The watch is LOUD! The analog watch I'm replacing is entirely quiet except when brought to the ear. This one... I hear it from feet away. My husband hears it from feet away too. I've tried two of the same model and they're equally loud. It gets distracting for me. Not at night, as the blankets mute it up, but during the day. Holding my phone typing this up, or during work, conversations... even when driving I hear it over the engine and traffic noise, because the driving position brings it closer to me, tick tick tick tick. It's impressive how that sound can carry.
I've read on the internet a few people saying they improved the noise of their Timex watches by removing the hand that does the seconds. I love having it but I might just get desperate enough to try this little operation. This is the perfect watch for me except for that problem. So sad.
(EDIT: I did end up trying to remove the seconds hand. It did quiet down the watch. Unfortunately, I also broke the watch in the process of putting it back together. I've decided to cut my losses and I'm now wearing a Fossil Carlie Mini (roughly $100, and available on Amazon, entirely quiet). Doesn't light up or have full numerals but what can you do. I wear a cheap digital that can light up at night and make do.)
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Reviewed in the United States on April 17, 2026
★★★★★ 5
Love it
Size: 30mm, Color: Pink/Rose Gold-Tone/Cream/30mm, Size: 30mm, Color: Pink/Rose Gold-Tone/Cream/30mm
This is a great simple watch. It fits my wrist well and is very comfortable. I really like the champagne/pink band along with the rose gold face. I didn’t realize if you push the button on the side of the face that it lights up for easy reading in the dark. Nice detail! Feels like really good quality! I hope it lasts me a long time.
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Reviewed in the United States on March 17, 2026
★★★★★ 5
Beautiful and reliable!
Size: 30mm, Color: Pink/Rose Gold-Tone/Cream/30mm
I just love it. It is beautiful, comfortable and I love the back light! I tried two or three smart watches that were reasonably priced and none of them worked well. I did not want to spend several hundred dollars for a watch. I finally decided on a good reliable watch and chose time mix. I’m really glad I did.
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Reviewed in the United States on April 6, 2026
★★★★★ 5
Nice watch!!
Size: 30mm, Color: Pink/Rose Gold-Tone/Cream/30mm
Works great! I've got a larger wrist and it fits me perfectly! I love the Indiglow feature! I can see what time it is in the middle of the night!!
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Reviewed in the United States on April 19, 2026
★★★★★ 5
Very nice watch
Size: 30mm, Color: Black/Gold-Tone/Cream/30mm
Very nice Timex watch reasonably priced.
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Reviewed in the United States on May 8, 2026